Restriction map for pF1KB9038
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AflII       AflIII      AgeI        
AhdI        AocI        Aor51HI     ApaI        ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AvaI        AviII       AvrII       BbrPI       BbvCI       
BciVI       BcnI        BfrI        BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaWI       
BseAI       BsiWI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrBI       BsrDI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        Eam1105I    
Eco47III    Eco81I      EcoNI       EcoO65I     EcoRI       
EcoT22I     FseI        FspI        HgaI        HindIII     
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        MvnI        NaeI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
SspBI       SspI        SwaI        Tsp509I     XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AcyI          325  GGCGCC               G[GA]^CG[CT]C
Alw44I         13  GTGCAC               G^TGCAC
AlwNI         428  CAGGCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI          13  GTGCAC               G^TGCAC
AspI         1249  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1031  GGATCC               G^GATCC
BbeI          325  GGCGCC               GGCGC^C
BcgI           38  GCAACCAGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI           68  TGATCA               T^GATCA
BfaI          202  CTAG                 C^TAG
BglI          386  GCCTGCAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         248  AGATCT               A^GATCT
BsaHI         325  GGCGCC               G[GA]^CG[CT]C
BsaI          592  GAGACC               GGTCTC(1/5)
BsgI          997  GTGCAG               GTGCAG(16/14)
BsiEI         921  CGGCCG               CG[GA][CT]^CG
BspHI         488  TCATGA               T^CATGA
BsrFI          97  ACCGGC               [GA]^CCGG[CT]
BstXI         188  CCATCCTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I         97  ACCGGC               [GA]^CCGG[CT]
DraIII       1070  CACCTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          529  GACACCGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          921  CGGCCG               C^GGCCG
EclXI         921  CGGCCG               C^GGCCG
Eco52I        921  CGGCCG               C^GGCCG
EcoRV         625  GATATC               GAT^ATC
EcoT14I       215  CCATGG               C^C[AT][AT]GG
FbaI           68  TGATCA               T^GATCA
HaeII         325  GGCGCC               [GA]GCGC^[CT]
Hin1I         325  GGCGCC               G[GA]^CG[CT]C
HincII        763  GTCAAC               GT[CT]^[GA]AC
HindII        763  GTCAAC               GT[CT]^[GA]AC
KasI          325  GGCGCC               G^GCGCC
MaeI          202  CTAG                 C^TAG
MseI          992  TTAA                 T^TAA
NarI          325  GGCGCC               GG^CGCC
NcoI          215  CCATGG               C^CATGG
PflFI        1249  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          220  GACTC                GAGTC(4/5)
PpuMI         234  AGGTCCT              [GA]G^G[AT]CC[CT]
PshAI        1050  GACAGCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          488  TCATGA               T^CATGA
SapI         1128  GCTCTTC              GCTCTTC(1/4)
SbfI          387  CCTGCAGG             CCTGCA^GG
SfoI          325  GGCGCC               GGC^GCC
SmlI         1081  CTCAAG               C^T[CT][GA]AG
Sse8387I      387  CCTGCAGG             CCTGCA^GG
StuI         1175  AGGCCT               AGG^CCT
StyI          215  CCATGG               C^C[AT][AT]GG
TfiI          340  GATTC                G^A[AT]TC
Tru9I         992  TTAA                 T^TAA
Tth111I      1249  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          201  TCTAGA               T^CTAGA
XcmI          887  CCAAGAACATGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

Asp700I       492  GAATACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1173  GAAGGCCTTC          
BalI          243  TGGCCA               TGG^CCA
              729  TGGCCA              
BbsI          877  GTCTTC               GAAGAC(2/6)
              946  GTCTTC              
BpuAI         877  GTCTTC               GAAGAC(2/6)
              946  GTCTTC              
BsmBI         784  CGTCTC               CGTCTC(1/5)
             1254  CGTCTC              
BssSI         182  CTCGTG               CACGAG(-5/-1)
             1073  CTCGTG              
CfoI          326  GCGC                 GCG^C
             1137  GCGC                
DraII         234  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              614  AGGCCCT             
EcoO109I      234  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              614  AGGCCCT             
FauI          914  CCCGC                CCCGC(4/6)
              419  GCGGG               
HhaI          326  GCGC                 GCG^C
             1137  GCGC                
HinP1I        326  GCGC                 G^CGC
             1137  GCGC                
HinfI         220  GACTC                G^A[ACGT]TC
              340  GATTC               
MaeII         783  ACGT                 A^CGT
              995  ACGT                
MluNI         243  TGGCCA               TGG^CCA
              729  TGGCCA              
MscI          243  TGGCCA               TGG^CCA
              729  TGGCCA              
MspA1I        127  CAGCTG               C[AC]G^C[GT]G
              400  CAGCTG              
PstI          124  CTGCAG               CTGCA^G
              388  CTGCAG              
PvuII         127  CAGCTG               CAG^CTG
              400  CAGCTG              
SacI          845  GAGCTC               GAGCT^C
             1126  GAGCTC              
SfcI          124  CTGCAG               C^T[GA][CT]AG
              388  CTGCAG              
XmnI          492  GAATACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1173  GAAGGCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
AvaII       BanI        BanII       BbvI        BmyI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DsaI        EaeI        
EarI        EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HpaII       HphI        ItaI        Ksp632I     
MaeIII      MboI        MboII       MflI        MnlI        
MslI        MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PflMI       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       TaqI        TseI        
Tsp45I      TspRI       TthHB8I     Van91I      XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com