(Circular) (Six-base) MAPSORT of: pF1KB9000.seq Check: 9490 from: 1 to: 4367 pF1KB9000 4367 bp With 182 enzymes: SgfI * June 20, 2008 10:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 489 503 489 Size: 14 4353 AatII G_ACGT'C Cuts at: 358 358 Size: 4367 AccI GT'mk_AC Cuts at: 1405 1405 Size: 4367 Acc65I G'GTAC_C Cuts at: 805 1383 805 Size: 578 3789 AclI AA'CG_TT Cuts at: 4207 4207 Size: 4367 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2192 2624 3371 3639 2192 Size: 432 747 268 2920 Fragments arranged by size: 2920 747 432 268 AflIII A'CryG_T Cuts at: 1151 1719 2823 3800 1151 Size: 568 1104 977 1718 Fragments arranged by size: 1718 1104 977 568 AgeI A'CCGG_T Cuts at: 2755 2755 Size: 4367 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 340 340 Size: 4367 AlwNI CAG_nnn'CTG Cuts at: 423 1672 3239 423 Size: 1249 1567 1551 Fragments arranged by size: 1567 1551 1249 ApaI G_GGCC'C Cuts at: 140 140 Size: 4367 ApaLI G'TGCA_C Cuts at: 3137 3137 Size: 4367 ApoI r'AATT_y Cuts at: 1222 1342 1371 3720 3976 1222 Size: 120 29 2349 256 1613 Fragments arranged by size: 2349 1613 256 120 29 AseI AT'TA_AT Cuts at: 20 20 Size: 4367 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4367 AvaI C'yCGr_G Cuts at: 141 211 956 1387 141 Size: 70 745 431 3121 Fragments arranged by size: 3121 745 431 70 BamHI G'GATC_C Cuts at: 1056 1392 1056 Size: 336 4031 BanI G'GyrC_C Cuts at: 786 805 1020 1383 1797 2045 2080 786 Size: 19 215 363 414 248 35 3073 Fragments arranged by size: 3073 414 363 248 215 35 19 BanII G_rGCy'C Cuts at: 140 1381 2411 140 Size: 1241 1030 2096 Fragments arranged by size: 2096 1241 1030 BbeI G_GCGC'C Cuts at: 790 1024 1801 2049 790 Size: 234 777 248 3108 Fragments arranged by size: 3108 777 248 234 BbsI GAAGACnn'nnnn_ Cuts at: 663 862 1263 663 Size: 199 401 3767 Fragments arranged by size: 3767 401 199 BbvCI CC'TCA_GC Cuts at: 385 385 Size: 4367 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 222 256 90 Size: 34 98 34 4201 Fragments arranged by size: 4201 98 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2265 3026 4367 2265 Size: 761 1341 2265 Fragments arranged by size: 2265 1341 761 BclI T'GATC_A Cuts at: 1887 1887 Size: 4367 BglI GCCn_nnn'nGGC Cuts at: 280 400 280 Size: 120 4247 BglII A'GATC_T Cuts at: 1882 3919 1882 Size: 2037 2330 BlpI GC'TnA_GC Cuts at: 637 649 1479 637 Size: 12 830 3525 Fragments arranged by size: 3525 830 12 Bme1580I G_kGCm'C Cuts at: 140 1992 2085 3141 140 Size: 1852 93 1056 1366 Fragments arranged by size: 1852 1366 1056 93 BmgBI CAC'GTC Cuts at: 240 240 Size: 4367 BmrI ACTGGGnnnn_n' Cuts at: 1123 1579 1660 1995 4139 1123 Size: 456 81 335 2144 1351 Fragments arranged by size: 2144 1351 456 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2734 2734 Size: 4367 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 135 286 135 Size: 151 4216 Bpu10I CC'TnA_GC Cuts at: 385 385 Size: 4367 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 562 1543 2422 2914 3212 562 Size: 981 879 492 298 1717 Fragments arranged by size: 1717 981 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4071 4071 Size: 4367 BsaAI yAC'GTr Cuts at: 756 1720 756 Size: 964 3403 BsaBI GATnn'nnATC Cuts at: 6 1881 3918 6 Size: 1875 2037 455 Fragments arranged by size: 2037 1875 455 BsaHI Gr'CG_yC Cuts at: 343 355 787 862 1021 1798 2046 2748 4244 Size: 12 432 75 159 777 248 702 1496 Cuts at: 4244 343 Size: 466 Fragments arranged by size: 1496 777 702 466 432 248 159 75 12 BsaWI w'CCGG_w Cuts at: 338 1556 2077 2755 3029 3176 3587 338 Size: 1218 521 678 274 147 411 1118 Fragments arranged by size: 1218 1118 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 265 265 Size: 4367 BseYI C'CCAG_C Cuts at: 306 437 716 1016 1792 2550 3127 3623 306 Size: 131 279 300 776 758 577 496 1050 Fragments arranged by size: 1050 776 758 577 496 300 279 131 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 319 902 319 Size: 583 3784 BsiEI CG_ry'CG Cuts at: 104 552 626 1909 1955 3163 104 Size: 448 74 1283 46 1208 1308 Fragments arranged by size: 1308 1283 1208 448 74 46 BsiHKAI G_wGCw'C Cuts at: 655 1381 2159 2349 3141 655 Size: 726 778 190 792 1881 Fragments arranged by size: 1881 792 778 726 190 BsmI GAATG_Cn' Cuts at: 409 409 Size: 4367 BsmBI CGTCTCn'nnnn_ Cuts at: 118 118 Size: 4367 Bsp1286I G_dGCh'C Cuts at: 140 655 1381 1992 2085 2159 2349 2411 3141 Size: 515 726 611 93 74 190 62 730 Cuts at: 3141 140 Size: 1366 Fragments arranged by size: 1366 730 726 611 515 190 93 74 62 BspEI T'CCGG_A Cuts at: 3587 3587 Size: 4367 BspHI T'CATG_A Cuts at: 539 614 3543 539 Size: 75 2929 1363 Fragments arranged by size: 2929 1363 75 BspMI ACCTGCnnnn'nnnn_ Cuts at: 489 503 817 1417 1631 1933 2314 489 Size: 14 314 600 214 302 381 2542 Fragments arranged by size: 2542 600 381 314 302 214 14 BsrBI CCG'CTC Cuts at: 50 576 605 2659 2713 4197 4362 50 Size: 526 29 2054 54 1484 165 55 Fragments arranged by size: 2054 1484 526 165 55 54 29 BsrDI GCAATG_nn' Cuts at: 2279 2279 Size: 4367 BsrFI r'CCGG_y Cuts at: 121 175 2365 2546 2755 121 Size: 54 2190 181 209 1733 Fragments arranged by size: 2190 1733 209 181 54 BssHII G'CGCG_C Cuts at: 598 598 Size: 4367 BssSI C'ACGA_G Cuts at: 746 2638 2996 746 Size: 1892 358 2117 Fragments arranged by size: 2117 1892 358 BstAPI GCAn_nnn'nTGC Cuts at: 1744 1744 Size: 4367 BstBI TT'CG_AA Cuts at: 2728 2728 Size: 4367 BstYI r'GATC_y Cuts at: 1 965 1056 1392 1859 1882 2217 2463 3475 Size: 964 91 336 467 23 335 246 1012 Cuts at: 3475 3561 3573 3919 1 Size: 86 12 346 449 Fragments arranged by size: 1012 964 467 449 346 336 335 246 91 86 23 12 BtgI C'CryG_G Cuts at: 157 404 459 791 849 157 Size: 247 55 332 58 3675 Fragments arranged by size: 3675 332 247 58 55 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 543 2317 2498 2661 543 Size: 1774 181 163 2249 Fragments arranged by size: 2249 1774 181 163 BtsI GCAGTG_nn' Cuts at: 1103 1610 1103 Size: 507 3860 DraI TTT'AAA Cuts at: 1366 1366 Size: 4367 DraIII CAC_nnn'GTG Cuts at: 931 931 Size: 4367 DrdI GACnn_nn'nnGTC Cuts at: 2073 2931 2073 Size: 858 3509 EaeI y'GGCC_r Cuts at: 170 257 299 392 401 623 1164 1952 2126 Size: 87 42 93 9 222 541 788 174 Cuts at: 2126 2517 2544 3889 170 Size: 391 27 1345 648 Fragments arranged by size: 1345 788 648 541 391 222 174 93 87 42 27 9 EagI C'GGCC_G Cuts at: 623 1952 623 Size: 1329 3038 EarI CTCTTCn'nnn_ Cuts at: 376 2390 2600 376 Size: 2014 210 2143 Fragments arranged by size: 2143 2014 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 108 2575 2885 3031 4177 108 Size: 2467 310 146 1146 298 Fragments arranged by size: 2467 1146 310 298 146 EcoICRI GAG'CTC Cuts at: 1379 1379 Size: 4367 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 135 286 2192 2624 3371 3639 135 Size: 151 1906 432 747 268 863 Fragments arranged by size: 1906 863 747 432 268 151 EcoNI CCTnn'n_nnAGG Cuts at: 214 214 Size: 4367 EcoO109I rG'GnC_Cy Cuts at: 136 137 1506 136 Size: 1 1369 2997 Fragments arranged by size: 2997 1369 1 EcoRI G'AATT_C Cuts at: 1371 1371 Size: 4367 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1280 2386 1280 Size: 1106 3261 FspI TGC'GCA Cuts at: 244 2148 244 Size: 1904 2463 HaeII r_GCGC'y Cuts at: 182 790 1024 1801 2049 3071 4050 182 Size: 608 234 777 248 1022 979 499 Fragments arranged by size: 1022 979 777 608 499 248 234 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1048 1080 2209 2239 2241 2271 1048 Size: 32 1129 30 2 30 3144 Fragments arranged by size: 3144 1129 32 30 30 2 HincII GTy'rAC Cuts at: 1406 1406 Size: 4367 HindIII A'AGCT_T Cuts at: 1191 1191 Size: 4367 KasI G'GCGC_C Cuts at: 786 1020 1797 2045 786 Size: 234 777 248 3108 Fragments arranged by size: 3108 777 248 234 KpnI G_GTAC'C Cuts at: 809 1387 809 Size: 578 3789 MluI A'CGCG_T Cuts at: 3800 3800 Size: 4367 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 402 3038 3222 402 Size: 2636 184 1547 Fragments arranged by size: 2636 1547 184 MscI TGG'CCA Cuts at: 172 259 1166 2128 172 Size: 87 907 962 2411 Fragments arranged by size: 2411 962 907 87 MslI CAynn'nnrTG Cuts at: 562 781 1039 2356 2483 562 Size: 219 258 1317 127 2446 Fragments arranged by size: 2446 1317 258 219 127 MspA1I CmG'CkG Cuts at: 461 851 1478 1792 2152 3165 3410 461 Size: 390 627 314 360 1013 245 1418 Fragments arranged by size: 1418 1013 627 390 360 314 245 NaeI GCC'GGC Cuts at: 2548 2548 Size: 4367 NarI GG'CG_CC Cuts at: 787 1021 1798 2046 787 Size: 234 777 248 3108 Fragments arranged by size: 3108 777 248 234 NcoI C'CATG_G Cuts at: 157 157 Size: 4367 NgoMIV G'CCGG_C Cuts at: 2546 2546 Size: 4367 NotI GC'GGCC_GC Cuts at: 623 623 Size: 4367 NspI r_CATG'y Cuts at: 366 513 531 1155 1420 1920 2827 366 Size: 147 18 624 265 500 907 1906 Fragments arranged by size: 1906 907 624 500 265 147 18 PasI CC'CwG_GG Cuts at: 737 737 Size: 4367 PciI A'CATG_T Cuts at: 1151 2823 1151 Size: 1672 2695 PflMI CCAn_nnn'nTGG Cuts at: 158 158 Size: 4367 PmeI GTTT'AAAC Cuts at: 1366 1366 Size: 4367 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3506 3538 3506 Size: 32 4335 PspOMI G'GGCC_C Cuts at: 136 136 Size: 4367 PstI C_TGCA'G Cuts at: 500 1414 2099 500 Size: 914 685 2768 Fragments arranged by size: 2768 914 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 4367 PvuII CAG'CTG Cuts at: 1792 2152 1792 Size: 360 4007 RsrII CG'GwC_CG Cuts at: 2562 2562 Size: 4367 SacI G_AGCT'C Cuts at: 1381 1381 Size: 4367 SacII CC_GC'GG Cuts at: 462 852 462 Size: 390 3977 SalI G'TCGA_C Cuts at: 1404 1404 Size: 4367 SapI GCTCTTCn'nnn_ Cuts at: 2390 2600 2390 Size: 210 4157 SbfI CC_TGCA'GG Cuts at: 500 1414 500 Size: 914 3453 ScaI AGT'ACT Cuts at: 664 3884 664 Size: 3220 1147 SfcI C'TryA_G Cuts at: 33 496 1410 2095 3088 3279 33 Size: 463 914 685 993 191 1121 Fragments arranged by size: 1121 993 914 685 463 191 SfiI GGCCn_nnn'nGGCC Cuts at: 400 400 Size: 4367 SfoI GGC'GCC Cuts at: 788 1022 1799 2047 788 Size: 234 777 248 3108 Fragments arranged by size: 3108 777 248 234 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4367 SgrAI Cr'CCGG_yG Cuts at: 175 175 Size: 4367 SmaI CCC'GGG Cuts at: 958 1389 958 Size: 431 3936 SmlI C'TyrA_G Cuts at: 577 1522 2437 2929 3191 577 Size: 945 915 492 262 1753 Fragments arranged by size: 1753 945 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4367 SphI G_CATG'C Cuts at: 366 1420 1920 366 Size: 1054 500 2813 Fragments arranged by size: 2813 1054 500 StyI C'CwwG_G Cuts at: 157 701 1501 1855 157 Size: 544 800 354 2669 Fragments arranged by size: 2669 800 544 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 621 1090 1950 2083 2542 2751 621 Size: 469 860 133 459 209 2237 Fragments arranged by size: 2237 860 469 459 209 133 TatI w'GTAC_w Cuts at: 662 3882 662 Size: 3220 1147 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3884 3916 3884 Size: 32 4335 Tth111I GACn'n_nGTC Cuts at: 861 2164 861 Size: 1303 3064 XbaI T'CTAG_A Cuts at: 1398 1398 Size: 4367 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1862 1862 Size: 4367 XmaI C'CCGG_G Cuts at: 956 1387 956 Size: 431 3936 ZraI GAC'GTC Cuts at: 356 356 Size: 4367 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PciI PflMI PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI Enzymes that do not cut: AfeI AflII AhdI AleI AloI AscI AvrII BaeI BfrBI BmtI BsaXI BsiWI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HpaI MfeI NdeI NheI NruI NsiI PacI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SanDI SexAI SnaBI SrfI SspI StuI SwaI XhoI XmnI