(Linear) (Six-base) MAPSORT of: KIBB9000.seq Check: 8198 from: 1 to: 1254 KIBB9000.orf With 182 enzymes: SgfI * March 3, 2008 11:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 381 395 1254 Size: 381 14 859 Fragments arranged by size: 859 381 14 AatII G_ACGT'C Cuts at: 0 250 1254 Size: 250 1004 Acc65I G'GTAC_C Cuts at: 0 697 1254 Size: 697 557 AflIII A'CryG_T Cuts at: 0 1043 1254 Size: 1043 211 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 232 1254 Size: 232 1022 AlwNI CAG_nnn'CTG Cuts at: 0 315 1254 Size: 315 939 ApaI G_GGCC'C Cuts at: 0 32 1254 Size: 32 1222 ApoI r'AATT_y Cuts at: 0 1114 1234 1254 Size: 1114 120 20 Fragments arranged by size: 1114 120 20 AvaI C'yCGr_G Cuts at: 0 33 103 848 1254 Size: 33 70 745 406 Fragments arranged by size: 745 406 70 33 BamHI G'GATC_C Cuts at: 0 948 1254 Size: 948 306 BanI G'GyrC_C Cuts at: 0 678 697 912 1254 Size: 678 19 215 342 Fragments arranged by size: 678 342 215 19 BanII G_rGCy'C Cuts at: 0 32 1254 Size: 32 1222 BbeI G_GCGC'C Cuts at: 0 682 916 1254 Size: 682 234 338 Fragments arranged by size: 682 338 234 BbsI GAAGACnn'nnnn_ Cuts at: 0 555 754 1155 1254 Size: 555 199 401 99 Fragments arranged by size: 555 401 199 99 BbvCI CC'TCA_GC Cuts at: 0 277 1254 Size: 277 977 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 114 148 1254 Size: 114 34 1106 Fragments arranged by size: 1106 114 34 BglI GCCn_nnn'nGGC Cuts at: 0 172 292 1254 Size: 172 120 962 Fragments arranged by size: 962 172 120 BlpI GC'TnA_GC Cuts at: 0 529 541 1254 Size: 529 12 713 Fragments arranged by size: 713 529 12 Bme1580I G_kGCm'C Cuts at: 0 32 1254 Size: 32 1222 BmgBI CAC'GTC Cuts at: 0 132 1254 Size: 132 1122 BmrI ACTGGGnnnn_n' Cuts at: 0 1015 1254 Size: 1015 239 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 27 178 1254 Size: 27 151 1076 Fragments arranged by size: 1076 151 27 Bpu10I CC'TnA_GC Cuts at: 0 277 1254 Size: 277 977 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 454 1254 Size: 454 800 BsaAI yAC'GTr Cuts at: 0 648 1254 Size: 648 606 BsaHI Gr'CG_yC Cuts at: 0 235 247 679 754 913 1254 Size: 235 12 432 75 159 341 Fragments arranged by size: 432 341 235 159 75 12 BsaWI w'CCGG_w Cuts at: 0 230 1254 Size: 230 1024 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 157 1254 Size: 157 1097 BseYI C'CCAG_C Cuts at: 0 198 329 608 908 1254 Size: 198 131 279 300 346 Fragments arranged by size: 346 300 279 198 131 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 211 794 1254 Size: 211 583 460 Fragments arranged by size: 583 460 211 BsiEI CG_ry'CG Cuts at: 0 444 518 1254 Size: 444 74 736 Fragments arranged by size: 736 444 74 BsiHKAI G_wGCw'C Cuts at: 0 547 1254 Size: 547 707 BsmI GAATG_Cn' Cuts at: 0 301 1254 Size: 301 953 BsmBI CGTCTCn'nnnn_ Cuts at: 0 10 1254 Size: 10 1244 Bsp1286I G_dGCh'C Cuts at: 0 32 547 1254 Size: 32 515 707 Fragments arranged by size: 707 515 32 BspHI T'CATG_A Cuts at: 0 431 506 1254 Size: 431 75 748 Fragments arranged by size: 748 431 75 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 381 395 709 1254 Size: 381 14 314 545 Fragments arranged by size: 545 381 314 14 BsrBI CCG'CTC Cuts at: 0 468 497 1254 Size: 468 29 757 Fragments arranged by size: 757 468 29 BsrFI r'CCGG_y Cuts at: 0 13 67 1254 Size: 13 54 1187 Fragments arranged by size: 1187 54 13 BssHII G'CGCG_C Cuts at: 0 490 1254 Size: 490 764 BssSI C'ACGA_G Cuts at: 0 638 1254 Size: 638 616 BstYI r'GATC_y Cuts at: 0 857 948 1254 Size: 857 91 306 Fragments arranged by size: 857 306 91 BtgI C'CryG_G Cuts at: 0 49 296 351 683 741 1254 Size: 49 247 55 332 58 513 Fragments arranged by size: 513 332 247 58 55 49 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 435 1254 Size: 435 819 BtsI GCAGTG_nn' Cuts at: 0 995 1254 Size: 995 259 DraIII CAC_nnn'GTG Cuts at: 0 823 1254 Size: 823 431 EaeI y'GGCC_r Cuts at: 0 62 149 191 284 293 515 1056 1254 Size: 62 87 42 93 9 222 541 198 Fragments arranged by size: 541 222 198 93 87 62 42 9 EagI C'GGCC_G Cuts at: 0 515 1254 Size: 515 739 EarI CTCTTCn'nnn_ Cuts at: 0 268 1254 Size: 268 986 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 27 178 1254 Size: 27 151 1076 Fragments arranged by size: 1076 151 27 EcoNI CCTnn'n_nnAGG Cuts at: 0 106 1254 Size: 106 1148 EcoO109I rG'GnC_Cy Cuts at: 0 28 29 1254 Size: 28 1 1225 Fragments arranged by size: 1225 28 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1172 1254 Size: 1172 82 FspI TGC'GCA Cuts at: 0 136 1254 Size: 136 1118 HaeII r_GCGC'y Cuts at: 0 74 682 916 1254 Size: 74 608 234 338 Fragments arranged by size: 608 338 234 74 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 940 972 1254 Size: 940 32 282 Fragments arranged by size: 940 282 32 HindIII A'AGCT_T Cuts at: 0 1083 1254 Size: 1083 171 KasI G'GCGC_C Cuts at: 0 678 912 1254 Size: 678 234 342 Fragments arranged by size: 678 342 234 KpnI G_GTAC'C Cuts at: 0 701 1254 Size: 701 553 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 294 1254 Size: 294 960 MscI TGG'CCA Cuts at: 0 64 151 1058 1254 Size: 64 87 907 196 Fragments arranged by size: 907 196 87 64 MslI CAynn'nnrTG Cuts at: 0 454 673 931 1254 Size: 454 219 258 323 Fragments arranged by size: 454 323 258 219 MspA1I CmG'CkG Cuts at: 0 353 743 1254 Size: 353 390 511 Fragments arranged by size: 511 390 353 NarI GG'CG_CC Cuts at: 0 679 913 1254 Size: 679 234 341 Fragments arranged by size: 679 341 234 NcoI C'CATG_G Cuts at: 0 49 1254 Size: 49 1205 NotI GC'GGCC_GC Cuts at: 0 515 1254 Size: 515 739 NspI r_CATG'y Cuts at: 0 258 405 423 1047 1254 Size: 258 147 18 624 207 Fragments arranged by size: 624 258 207 147 18 PasI CC'CwG_GG Cuts at: 0 629 1254 Size: 629 625 PciI A'CATG_T Cuts at: 0 1043 1254 Size: 1043 211 PflMI CCAn_nnn'nTGG Cuts at: 0 50 1254 Size: 50 1204 PspOMI G'GGCC_C Cuts at: 0 28 1254 Size: 28 1226 PstI C_TGCA'G Cuts at: 0 392 1254 Size: 392 862 SacII CC_GC'GG Cuts at: 0 354 744 1254 Size: 354 390 510 Fragments arranged by size: 510 390 354 SbfI CC_TGCA'GG Cuts at: 0 392 1254 Size: 392 862 ScaI AGT'ACT Cuts at: 0 556 1254 Size: 556 698 SfcI C'TryA_G Cuts at: 0 388 1254 Size: 388 866 SfiI GGCCn_nnn'nGGCC Cuts at: 0 292 1254 Size: 292 962 SfoI GGC'GCC Cuts at: 0 680 914 1254 Size: 680 234 340 Fragments arranged by size: 680 340 234 SgrAI Cr'CCGG_yG Cuts at: 0 67 1254 Size: 67 1187 SmaI CCC'GGG Cuts at: 0 850 1254 Size: 850 404 SmlI C'TyrA_G Cuts at: 0 469 1254 Size: 469 785 SphI G_CATG'C Cuts at: 0 258 1254 Size: 258 996 StyI C'CwwG_G Cuts at: 0 49 593 1254 Size: 49 544 661 Fragments arranged by size: 661 544 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 513 982 1254 Size: 513 469 272 Fragments arranged by size: 513 469 272 TatI w'GTAC_w Cuts at: 0 554 1254 Size: 554 700 Tth111I GACn'n_nGTC Cuts at: 0 753 1254 Size: 753 501 XmaI C'CCGG_G Cuts at: 0 848 1254 Size: 848 406 ZraI GAC'GTC Cuts at: 0 248 1254 Size: 248 1006 Enzymes that do cut: AarI AatII Acc65I AflIII AlfI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BssSI BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HindIII KasI KpnI MmeI MscI MslI MspA1I NarI NcoI NotI NspI PasI PciI PflMI PspOMI PstI SacII SbfI ScaI SfcI SfiI SfoI SgrAI SmaI SmlI SphI StyI TaqII TatI Tth111I XmaI ZraI Enzymes that do not cut: AccI AclI AcuI AfeI AflII AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI BglII BmtI BplI BsaI BsaBI BsaXI BsiWI BspEI BsrDI BsrGI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DrdI EciI EcoICRI EcoRI EcoRV FseI FspAI HincII HpaI MfeI MluI NaeI NdeI NgoMIV NheI NruI NsiI PacI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI SapI SexAI SgfI SnaBI SpeI SrfI SspI StuI SwaI TstI XbaI XcmI XhoI XmnI