Restriction map for pF1KB8979
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       AccI        AccII       AclI        AcyI        
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsgI        BsiEI       BsiWI       BsmBI       
BsoBI       Bsp1407I    BspDI       BspHI       BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       FauI        FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KspI        MaeI        MaeII       MamI        MfeI        
MluI        MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        SwaI        TaqI        
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

Acc65I        257  GGTACC               G^GTACC
AccIII        857  TCCGGA               T^CCGGA
AciI          570  CCGC                 CCGC(-3/-1)
AcsI          135  AAATTC               [GA]^AATT[CT]
AflII         273  CTTAAG               C^TTAAG
AlwNI          20  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          135  AAATTC               [GA]^AATT[CT]
Asp718I       257  GGTACC               G^GTACC
AspHI         125  GAGCTC               G[AT]GC[AT]^C
BanI          257  GGTACC               G^G[CT][GA]CC
BanII         125  GAGCTC               G[GA]GC[CT]^C
BfrI          273  CTTAAG               C^TTAAG
BsaWI         857  TCCGGA               [AT]^CCGG[AT]
BseAI         857  TCCGGA               T^CCGGA
BseRI         910  CTCCTC               GAGGAG(10/8)
BsiHKAI       125  GAGCTC               G[AT]GC[AT]^C
BsmAI         908  GTCTC                GTCTC(1/5)
BsmFI         325  GTCCC                GGGAC(10/14)
BsmI          799  GCATTC               GAATGC(1/-1)
BspEI         857  TCCGGA               T^CCGGA
DdeI         1056  CTTAG                C^T[ACGT]AG
DsaI          251  CCATGG               C^C[GA][CT]GG
EarI          145  GAAGAG               CTCTTC(1/4)
EcoT22I       869  ATGCAT               ATGCA^T
KpnI          257  GGTACC               GGTAC^C
Ksp632I       145  GAAGAG               CTCTTC(1/4)
MroI          857  TCCGGA               T^CCGGA
MspA1I         20  CAGCTG               C[AC]G^C[GT]G
NcoI          251  CCATGG               C^CATGG
NsiI          869  ATGCAT               ATGCA^T
PpuMI         488  AGGTCCT              [GA]G^G[AT]CC[CT]
PvuII          20  CAGCTG               CAG^CTG
SacI          125  GAGCTC               GAGCT^C
ScaI          277  AGTACT               AGT^ACT
SmlI          273  CTTAAG               C^T[CT][GA]AG
TfiI          809  GATTC                G^A[AT]TC
Tsp45I        340  GTGAC                ^GT[GC]AC
XcmI          296  CCATCTACCTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AflIII        527  ACATGT               A^C[GA][CT]GT
              637  ACATGT              
AlwI          371  GATCC                GGATC(4/5)
             1060  GATCC               
AvaII         365  GGACC                G^G[AT]CC
              489  GGTCC               
BalI          404  TGGCCA               TGG^CCA
              578  TGGCCA              
BmyI          125  GAGCTC               G[AGT]GC[ACT]^C
              207  GTGCCC              
Bsp1286I      125  GAGCTC               G[AGT]GC[ACT]^C
              207  GTGCCC              
BspLU11I      527  ACATGT               A^CATGT
              637  ACATGT              
BspMI         291  ACCTGC               ACCTGC(4/8)
              730  ACCTGC              
BsrDI         408  CATTGC               GCAATG(2/0)
              434  CATTGC              
BsrI          473  ACTGG                ACTGG(1/-1)
              645  ACTGG               
BstYI         370  AGATCC               [GA]^GATC[CT]
             1059  AGATCC              
DraII         488  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              605  GGGGCCT             
EcoO109I      488  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              605  GGGGCCT             
MflI          370  AGATCC               [GA]^GATC[CT]
             1059  AGATCC              
MluNI         404  TGGCCA               TGG^CCA
              578  TGGCCA              
MscI          404  TGGCCA               TGG^CCA
              578  TGGCCA              
MwoI           36  GCTTCTGTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              563  GCATCAACCGC         
NspI          527  ACATGT               [GA]CATG^[CT]
              637  ACATGT              
PleI          757  GAGTC                GAGTC(4/5)
              906  GAGTC               
SspI          701  AATATT               AAT^ATT
              952  AATATT              
XhoII         370  AGATCC               [GA]^GATC[CT]
             1059  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AfaI        AluI        BbvI        BcnI        BsaJI       
BsiYI       BslI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DpnII       EaeI        EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspI        MvaI        NciI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        Tru9I       TseI        Tsp509I     
TspRI       
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