Restriction map for pF1KB8952
 (Linear) (Six-base) MAPSORT of: KIBB8952.seq  Check: 2516  from: 1  to: 981



KIBB8952.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AccI GT'mk_AC

Cuts at:      0     606     981 
   Size:        606     375
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     833     981 
   Size:        833     148
 
ApaI G_GGCC'C

Cuts at:      0      93     427     981 
   Size:         93     334     554

  Fragments arranged by size: 

                 554     334      93
 
ApoI r'AATT_y

Cuts at:      0     731     780     918     981 
   Size:        731      49     138      63

  Fragments arranged by size: 

                 731     138      63      49
 
AvaI C'yCGr_G

Cuts at:      0      74     143     269     399     420     444     981 
   Size:         74      69     126     130      21      24     537

  Fragments arranged by size: 

                 537     130     126      74      69      24      21
 
AvrII C'CTAG_G

Cuts at:      0     180     981 
   Size:        180     801
 
BamHI G'GATC_C

Cuts at:      0     131     981 
   Size:        131     850
 
BanII G_rGCy'C

Cuts at:      0      93     388     427     981 
   Size:         93     295      39     554

  Fragments arranged by size: 

                 554     295      93      39
 
BglI GCCn_nnn'nGGC

Cuts at:      0     439     981 
   Size:        439     542
 
Bme1580I G_kGCm'C

Cuts at:      0      93     150     427     981 
   Size:         93      57     277     554

  Fragments arranged by size: 

                 554     277      93      57
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     391     981 
   Size:        206     185     590

  Fragments arranged by size: 

                 590     206     185
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      96     126     981 
   Size:         96      30     855

  Fragments arranged by size: 

                 855      96      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     257     955     981 
   Size:        257     698      26

  Fragments arranged by size: 

                 698     257      26
 
BseYI C'CCAG_C

Cuts at:      0     209     331     838     981 
   Size:        209     122     507     143

  Fragments arranged by size: 

                 507     209     143     122
 
BsiEI CG_ry'CG

Cuts at:      0     364     381     395     431     981 
   Size:        364      17      14      36     550

  Fragments arranged by size: 

                 550     364      36      17      14
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      15     981 
   Size:         15     966
 
Bsp1286I G_dGCh'C

Cuts at:      0      93     150     388     427     981 
   Size:         93      57     238      39     554

  Fragments arranged by size: 

                 554     238      93      57      39
 
BsrBI CCG'CTC

Cuts at:      0      33     404     981 
   Size:         33     371     577

  Fragments arranged by size: 

                 577     371      33
 
BsrFI r'CCGG_y

Cuts at:      0     359     394     463     981 
   Size:        359      35      69     518

  Fragments arranged by size: 

                 518     359      69      35
 
BstEII G'GTnAC_C

Cuts at:      0     102     981 
   Size:        102     879
 
BstYI r'GATC_y

Cuts at:      0     131     981 
   Size:        131     850
 
BstZ17I GTA'TAC

Cuts at:      0     607     981 
   Size:        607     374
 
Bsu36I CC'TnA_GG

Cuts at:      0     529     981 
   Size:        529     452
 
BtgI C'CryG_G

Cuts at:      0     107     251     506     585     981 
   Size:        107     144     255      79     396

  Fragments arranged by size: 

                 396     255     144     107      79
 
BtsI GCAGTG_nn'

Cuts at:      0     148     981 
   Size:        148     833
 
DraI TTT'AAA

Cuts at:      0     545     759     981 
   Size:        545     214     222

  Fragments arranged by size: 

                 545     222     214
 
EaeI y'GGCC_r

Cuts at:      0      45     361     378     428     516     588     981 
   Size:         45     316      17      50      88      72     393

  Fragments arranged by size: 

                 393     316      88      72      50      45      17
 
EagI C'GGCC_G

Cuts at:      0     361     378     428     981 
   Size:        361      17      50     553

  Fragments arranged by size: 

                 553     361      50      17
 
EarI CTCTTCn'nnn_

Cuts at:      0     169     981 
   Size:        169     812
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     104     981 
   Size:        104     877
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     391     833     981 
   Size:        206     185     442     148

  Fragments arranged by size: 

                 442     206     185     148
 
EcoO109I rG'GnC_Cy

Cuts at:      0      89      90     417     981 
   Size:         89       1     327     564

  Fragments arranged by size: 

                 564     327      89       1
 
EcoRI G'AATT_C

Cuts at:      0     731     981 
   Size:        731     250
 
HaeII r_GCGC'y

Cuts at:      0     280     981 
   Size:        280     701
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      96     128     981 
   Size:         96      32     853

  Fragments arranged by size: 

                 853      96      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     947     981 
   Size:        947      34
 
MscI TGG'CCA

Cuts at:      0     590     981 
   Size:        590     391
 
MslI CAynn'nnrTG

Cuts at:      0     614     981 
   Size:        614     367
 
MspA1I CmG'CkG

Cuts at:      0      24     327     498     508     981 
   Size:         24     303     171      10     473

  Fragments arranged by size: 

                 473     303     171      24      10
 
NaeI GCC'GGC

Cuts at:      0     465     981 
   Size:        465     516
 
NcoI C'CATG_G

Cuts at:      0     585     981 
   Size:        585     396
 
NgoMIV G'CCGG_C

Cuts at:      0     463     981 
   Size:        463     518
 
PfoI T'CCnGG_A

Cuts at:      0     134     981 
   Size:        134     847
 
PpuMI rG'GwC_Cy

Cuts at:      0     417     981 
   Size:        417     564
 
PshAI GACnn'nnGTC

Cuts at:      0       7     981 
   Size:          7     974
 
PspOMI G'GGCC_C

Cuts at:      0      89     423     981 
   Size:         89     334     558

  Fragments arranged by size: 

                 558     334      89
 
PstI C_TGCA'G

Cuts at:      0     456     981 
   Size:        456     525
 
PvuII CAG'CTG

Cuts at:      0     327     981 
   Size:        327     654
 
SacII CC_GC'GG

Cuts at:      0     509     981 
   Size:        509     472
 
SfcI C'TryA_G

Cuts at:      0     452     981 
   Size:        452     529
 
SmaI CCC'GGG

Cuts at:      0     145     422     981 
   Size:        145     277     559

  Fragments arranged by size: 

                 559     277     145
 
StyI C'CwwG_G

Cuts at:      0     180     437     585     981 
   Size:        180     257     148     396

  Fragments arranged by size: 

                 396     257     180     148
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      31     981 
   Size:         31     950
 
TatI w'GTAC_w

Cuts at:      0     948     981 
   Size:        948      33
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     598     981 
   Size:        598     383
 
XmaI C'CCGG_G

Cuts at:      0     143     420     981 
   Size:        143     277     561

  Fragments arranged by size: 

                 561     277     143
 

 Enzymes that do cut:

     AccI     AcuI     ApaI     ApoI     AvaI    AvrII    BamHI    BanII
     BglI Bme1580I     BpmI    BsaXI    BseRI    BseYI    BsiEI    BsmBI
 Bsp1286I    BsrBI    BsrFI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI
     BtsI     DraI     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I
    EcoRI    HaeII    Hin4I     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI   NgoMIV     PfoI    PpuMI    PshAI   PspOMI     PstI    PvuII
    SacII     SfcI     SmaI     StyI    TaqII     TatI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI     BaeI     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI     BsgI
  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI    BstXI    BtgZI     ClaI    CspCI
   DraIII     DrdI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NarI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XbaI     XhoI     XmnI     ZraI
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