Restriction map for pF1KB8945
 (Linear) (Six-base) MAPSORT of: KIBB8945.seq  Check: 9055  from: 1  to: 912



KIBB8945.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     212     723     912 
   Size:        212     511     189

  Fragments arranged by size: 

                 511     212     189
 
AccI GT'mk_AC

Cuts at:      0      20     912 
   Size:         20     892
 
Acc65I G'GTAC_C

Cuts at:      0     594     912 
   Size:        594     318
 
AflII C'TTAA_G

Cuts at:      0     313     912 
   Size:        313     599
 
AflIII A'CryG_T

Cuts at:      0     671     912 
   Size:        671     241
 
AhdI GACnn_n'nnGTC

Cuts at:      0     867     912 
   Size:        867      45
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     531     912 
   Size:        531     381
 
ApaLI G'TGCA_C

Cuts at:      0     835     912 
   Size:        835      77
 
ApoI r'AATT_y

Cuts at:      0     658     912 
   Size:        658     254
 
AvaI C'yCGr_G

Cuts at:      0      79     215     912 
   Size:         79     136     697

  Fragments arranged by size: 

                 697     136      79
 
BanI G'GyrC_C

Cuts at:      0     229     491     594     912 
   Size:        229     262     103     318

  Fragments arranged by size: 

                 318     262     229     103
 
BbeI G_GCGC'C

Cuts at:      0     233     912 
   Size:        233     679
 
BbvCI CC'TCA_GC

Cuts at:      0     879     912 
   Size:        879      33
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     196     230     804     838     912 
   Size:        196      34     574      34      74

  Fragments arranged by size: 

                 574     196      74      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     298     445     481     912 
   Size:        298     147      36     431

  Fragments arranged by size: 

                 431     298     147      36
 
Bme1580I G_kGCm'C

Cuts at:      0     184     303     494     790     839     912 
   Size:        184     119     191     296      49      73

  Fragments arranged by size: 

                 296     191     184     119      73      49
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     664     670     912 
   Size:        664       6     242

  Fragments arranged by size: 

                 664     242       6
 
Bpu10I CC'TnA_GC

Cuts at:      0     879     912 
   Size:        879      33
 
BsaBI GATnn'nnATC

Cuts at:      0     700     912 
   Size:        700     212
 
BsaHI Gr'CG_yC

Cuts at:      0     230     584     912 
   Size:        230     354     328

  Fragments arranged by size: 

                 354     328     230
 
BsaWI w'CCGG_w

Cuts at:      0     689     912 
   Size:        689     223
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     193     223     832     862     912 
   Size:        193      30     609      30      50

  Fragments arranged by size: 

                 609     193      50      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     201     912 
   Size:        201     711
 
BseYI C'CCAG_C

Cuts at:      0     422     912 
   Size:        422     490
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     190     501     912 
   Size:        190     311     411

  Fragments arranged by size: 

                 411     311     190
 
BsiEI CG_ry'CG

Cuts at:      0     440     476     912 
   Size:        440      36     436

  Fragments arranged by size: 

                 440     436      36
 
BsiHKAI G_wGCw'C

Cuts at:      0     839     912 
   Size:        839      73
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     158     912 
   Size:        158     754
 
Bsp1286I G_dGCh'C

Cuts at:      0     184     303     494     790     839     912 
   Size:        184     119     191     296      49      73

  Fragments arranged by size: 

                 296     191     184     119      73      49
 
BspEI T'CCGG_A

Cuts at:      0     689     912 
   Size:        689     223
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     212     723     912 
   Size:        212     511     189

  Fragments arranged by size: 

                 511     212     189
 
BsrBI CCG'CTC

Cuts at:      0     164     912 
   Size:        164     748
 
BsrFI r'CCGG_y

Cuts at:      0     232     873     912 
   Size:        232     641      39

  Fragments arranged by size: 

                 641     232      39
 
BssHII G'CGCG_C

Cuts at:      0     844     912 
   Size:        844      68
 
BstEII G'GTnAC_C

Cuts at:      0     258     912 
   Size:        258     654
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     430     912 
   Size:        430     482
 
BtgI C'CryG_G

Cuts at:      0     358     440     476     547     569     912 
   Size:        358      82      36      71      22     343

  Fragments arranged by size: 

                 358     343      82      71      36      22
 
BtsI GCAGTG_nn'

Cuts at:      0     282     301     558     912 
   Size:        282      19     257     354

  Fragments arranged by size: 

                 354     282     257      19
 
EaeI y'GGCC_r

Cuts at:      0     437     473     912 
   Size:        437      36     439

  Fragments arranged by size: 

                 439     437      36
 
EagI C'GGCC_G

Cuts at:      0     437     473     912 
   Size:        437      36     439

  Fragments arranged by size: 

                 439     437      36
 
EarI CTCTTCn'nnn_

Cuts at:      0     333     669     912 
   Size:        333     336     243

  Fragments arranged by size: 

                 336     333     243
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     556     912 
   Size:        556     356
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     664     670     912 
   Size:        664       6     242

  Fragments arranged by size: 

                 664     242       6
 
EcoRI G'AATT_C

Cuts at:      0     658     912 
   Size:        658     254
 
FspI TGC'GCA

Cuts at:      0     374     912 
   Size:        374     538
 
FspAI rTGC'GCAy

Cuts at:      0     374     912 
   Size:        374     538
 
HaeII r_GCGC'y

Cuts at:      0     233     912 
   Size:        233     679
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     786     818     847     853     879     885     912 
   Size:        786      32      29       6      26       6      27

  Fragments arranged by size: 

                 786      32      29      27      26       6       6
 
HincII GTy'rAC

Cuts at:      0      21     912 
   Size:         21     891
 
KasI G'GCGC_C

Cuts at:      0     229     912 
   Size:        229     683
 
KpnI G_GTAC'C

Cuts at:      0     598     912 
   Size:        598     314
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     692     704     912 
   Size:        692      12     208

  Fragments arranged by size: 

                 692     208      12
 
MspA1I CmG'CkG

Cuts at:      0     239     360     442     463     478     549     912 
   Size:        239     121      82      21      15      71     363

  Fragments arranged by size: 

                 363     239     121      82      71      21      15
 
NaeI GCC'GGC

Cuts at:      0     234     875     912 
   Size:        234     641      37

  Fragments arranged by size: 

                 641     234      37
 
NarI GG'CG_CC

Cuts at:      0     230     912 
   Size:        230     682
 
NcoI C'CATG_G

Cuts at:      0     569     912 
   Size:        569     343
 
NgoMIV G'CCGG_C

Cuts at:      0     232     873     912 
   Size:        232     641      39

  Fragments arranged by size: 

                 641     232      39
 
NotI GC'GGCC_GC

Cuts at:      0     473     912 
   Size:        473     439
 
NspI r_CATG'y

Cuts at:      0     111     675     912 
   Size:        111     564     237

  Fragments arranged by size: 

                 564     237     111
 
PciI A'CATG_T

Cuts at:      0     671     912 
   Size:        671     241
 
PfoI T'CCnGG_A

Cuts at:      0     641     912 
   Size:        641     271
 
SacII CC_GC'GG

Cuts at:      0     361     443     479     550     912 
   Size:        361      82      36      71     362

  Fragments arranged by size: 

                 362     361      82      71      36
 
SalI G'TCGA_C

Cuts at:      0      19     912 
   Size:         19     893
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     481     912 
   Size:        481     431
 
SfoI GGC'GCC

Cuts at:      0     231     912 
   Size:        231     681
 
SmaI CCC'GGG

Cuts at:      0      81     912 
   Size:         81     831
 
SmlI C'TyrA_G

Cuts at:      0     313     912 
   Size:        313     599
 
SphI G_CATG'C

Cuts at:      0     111     912 
   Size:        111     801
 
StyI C'CwwG_G

Cuts at:      0     485     569     912 
   Size:        485      84     343

  Fragments arranged by size: 

                 485     343      84
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     193     225     912 
   Size:        193      32     687

  Fragments arranged by size: 

                 687     193      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     534     598     912 
   Size:        534      64     314

  Fragments arranged by size: 

                 534     314      64
 
XmaI C'CCGG_G

Cuts at:      0      79     912 
   Size:         79     833
 

 Enzymes that do cut:

     AarI     AccI   Acc65I    AflII   AflIII     AhdI     AlfI    ApaLI
     ApoI     AvaI     BanI     BbeI    BbvCI     BcgI     BglI Bme1580I
     BpmI   Bpu10I    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI
    BsrFI   BssHII   BstEII    BstXI     BtgI     BtsI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoRI     FspI    FspAI    HaeII    Hin4I
   HincII     KasI     KpnI     MmeI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NotI     NspI     PciI     PfoI    SacII     SalI     SfiI
     SfoI     SmaI     SmlI     SphI     StyI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AclI     AcuI     AfeI     AgeI     AleI     AloI    AlwNI
     ApaI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII
     BbsI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsiWI     BsmI    BspHI
    BsrDI    BsrGI    BssSI   BstAPI    BstBI    BstYI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FalI     FseI  HindIII     HpaI     MfeI     MluI
     MscI     MslI     NdeI     NheI     NruI     NsiI     PacI     PasI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SgfI    SgrAI    SnaBI
     SpeI     SrfI     SspI     StuI     SwaI    TaqII     TatI  Tth111I
     XbaI     XhoI     XmnI     ZraI
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