Restriction map for pF1KB8938
##################################
# NO CUT ENZYMES (  157 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BclI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BsgI        BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DdeI        DpnII       
DraI        DraIII      DrdI        Eam1105I    Eco47III    
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeII       
MamI        MboI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MslI        MunI        
NarI        NcoI        NdeI        NdeII       NheI        
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacII       SalI        SapI        Sau3AI      
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StyI        SwaI        
Tth111I     XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI          587  GTAGAC               GT^[AC][GT]AC
AlwNI         578  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         534  GAGCTC               G[AT]GC[AT]^C
AvaI          112  CCCGAG               C^[CT]CG[GA]G
AvaII         513  GGACC                G^G[AT]CC
BciVI         412  GGATAC               GTATCC(6/5)
BglI          310  GCCTACCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          838  CTGGAG               CTGGAG(16/14)
BsaI          567  GAGACC               GGTCTC(1/5)
BsiHKAI       534  GAGCTC               G[AT]GC[AT]^C
BsmBI         539  CGTCTC               CGTCTC(1/5)
BsoBI         112  CCCGAG               C^[CT]CG[GA]G
BsrFI         175  GCCGGC               [GA]^CCGG[CT]
Cfr10I        175  GCCGGC               [GA]^CCGG[CT]
DraII         512  AGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI          559  CCGTGG               C^C[GA][CT]GG
EarI          593  GAAGAG               CTCTTC(1/4)
EcoO109I      512  AGGACCC              [GA]G^G[ACGT]CC[CT]
HaeII         169  AGCGCC               [GA]GCGC^[CT]
HinfI         680  GAATC                G^A[ACGT]TC
Ksp632I       593  GAAGAG               CTCTTC(1/4)
NaeI          175  GCCGGC               GCC^GGC
NgoMIV        175  GCCGGC               G^CCGGC
PflMI         831  CCAAGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         512  AGGACCC              [GA]G^G[AT]CC[CT]
PstI          151  CTGCAG               CTGCA^G
SacI          534  GAGCTC               GAGCT^C
ScaI          275  AGTACT               AGT^ACT
SfaNI         339  GCATC                GCATC(5/9)
SspI          502  AATATT               AAT^ATT
StuI          858  AGGCCT               AGG^CCT
TaqI          683  TCGA                 T^CGA
TfiI          680  GAATC                G^A[AT]TC
TspRI         495  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       683  TCGA                 T^CGA
Van91I        831  CCAAGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          627  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcsI          643  GAATTT               [GA]^AATT[CT]
              778  AAATTT              
ApoI          643  GAATTT               [GA]^AATT[CT]
              778  AAATTT              
BanII         394  GAGCCC               G[GA]GC[CT]^C
              534  GAGCTC              
BbsI          599  GAAGAC               GAAGAC(2/6)
              844  GAAGAC              
BfaI          628  CTAG                 C^TAG
              700  CTAG                
BmyI          394  GAGCCC               G[AGT]GC[ACT]^C
              534  GAGCTC              
BpuAI         599  GAAGAC               GAAGAC(2/6)
              844  GAAGAC              
BseRI         329  CTCCTC               GAGGAG(10/8)
              332  CTCCTC              
BsiEI         123  CGGCCG               CG[GA][CT]^CG
              302  CGGCCG              
BsmFI         238  GGGAC                GGGAC(10/14)
              222  GTCCC               
Bsp1286I      394  GAGCCC               G[AGT]GC[ACT]^C
              534  GAGCTC              
BsrI          241  ACTGG                ACTGG(1/-1)
              494  CCAGT               
BstF5I        340  CATCC                GGATG(2/0)
              563  GGATG               
Cfr13I        267  GGGCC                G^G[ACGT]CC
              513  GGACC               
EaeI          123  CGGCCG               [CT]^GGCC[GA]
              302  CGGCCG              
EagI          123  CGGCCG               C^GGCCG
              302  CGGCCG              
EclXI         123  CGGCCG               C^GGCCG
              302  CGGCCG              
Eco52I        123  CGGCCG               C^GGCCG
              302  CGGCCG              
FokI          340  CATCC                GGATG(9/13)
              563  GGATG               
HphI          801  GGTGA                GGTGA(8/7)
              707  TCACC               
MaeI          628  CTAG                 C^TAG
              700  CTAG                
MseI          526  TTAA                 T^TAA
              653  TTAA                
MspA1I         22  CCGCTG               C[AC]G^C[GT]G
              305  CCGCTG              
Sau96I        267  GGGCC                G^G[ACGT]CC
              513  GGACC               
SfcI          151  CTGCAG               C^T[GA][CT]AG
              609  CTACAG              
Tru9I         526  TTAA                 T^TAA
              653  TTAA                
Tsp509I       644  AATT                 ^AATT
              779  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        BbvI        
BcnI        BsaJI       BsiYI       BslI        BsmAI       
BssKI       BstNI       BstUI       Cac8I       CfoI        
EcoRII      FauI        Fnu4HI      HaeIII      HapII       
HhaI        HinP1I      HpaII       ItaI        MaeIII      
MboII       MnlI        MspI        MvaI        MvnI        
MwoI        NciI        NlaIV       RsaI        ScrFI       
TseI        Tsp45I      
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