Restriction map for pF1KB8938
 (Linear) (Six-base) MAPSORT of: KIBB8938.seq  Check: 3315  from: 1  to: 870



KIBB8938.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AccI GT'mk_AC

Cuts at:      0     588     870 
   Size:        588     282
 
AlwNI CAG_nnn'CTG

Cuts at:      0     583     870 
   Size:        583     287
 
ApoI r'AATT_y

Cuts at:      0     643     778     870 
   Size:        643     135      92

  Fragments arranged by size: 

                 643     135      92
 
AvaI C'yCGr_G

Cuts at:      0     112     870 
   Size:        112     758
 
BanII G_rGCy'C

Cuts at:      0     398     538     870 
   Size:        398     140     332

  Fragments arranged by size: 

                 398     332     140
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     606     851     870 
   Size:        606     245      19

  Fragments arranged by size: 

                 606     245      19
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     406     870 
   Size:        406     464
 
BglI GCCn_nnn'nGGC

Cuts at:      0     316     870 
   Size:        316     554
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     251     870 
   Size:        251     619
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     557     870 
   Size:        557     313
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     859     870 
   Size:        859      11
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     561     870 
   Size:        561     309
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     320     323     870 
   Size:        320       3     547

  Fragments arranged by size: 

                 547     320       3
 
BseYI C'CCAG_C

Cuts at:      0     449     870 
   Size:        449     421
 
BsiEI CG_ry'CG

Cuts at:      0     126     305     870 
   Size:        126     179     565

  Fragments arranged by size: 

                 565     179     126
 
BsiHKAI G_wGCw'C

Cuts at:      0     538     870 
   Size:        538     332
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     545     870 
   Size:        545     325
 
Bsp1286I G_dGCh'C

Cuts at:      0     398     538     870 
   Size:        398     140     332

  Fragments arranged by size: 

                 398     332     140
 
BsrFI r'CCGG_y

Cuts at:      0     175     870 
   Size:        175     695
 
BtgI C'CryG_G

Cuts at:      0     559     870 
   Size:        559     311
 
EaeI y'GGCC_r

Cuts at:      0     123     302     870 
   Size:        123     179     568

  Fragments arranged by size: 

                 568     179     123
 
EagI C'GGCC_G

Cuts at:      0     123     302     870 
   Size:        123     179     568

  Fragments arranged by size: 

                 568     179     123
 
EarI CTCTTCn'nnn_

Cuts at:      0     588     870 
   Size:        588     282
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     335     341     870 
   Size:        335       6     529

  Fragments arranged by size: 

                 529     335       6
 
EcoICRI GAG'CTC

Cuts at:      0     536     870 
   Size:        536     334
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     859     870 
   Size:        859      11
 
EcoO109I rG'GnC_Cy

Cuts at:      0     513     870 
   Size:        513     357
 
HaeII r_GCGC'y

Cuts at:      0     173     870 
   Size:        173     697
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     369     401     472     504     870 
   Size:        369      32      71      32     366

  Fragments arranged by size: 

                 369     366      71      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     614     870 
   Size:        614     256
 
MspA1I CmG'CkG

Cuts at:      0      24     307     870 
   Size:         24     283     563

  Fragments arranged by size: 

                 563     283      24
 
NaeI GCC'GGC

Cuts at:      0     177     870 
   Size:        177     693
 
NgoMIV G'CCGG_C

Cuts at:      0     175     870 
   Size:        175     695
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     837     870 
   Size:        837      33
 
PfoI T'CCnGG_A

Cuts at:      0     235     870 
   Size:        235     635
 
PpuMI rG'GwC_Cy

Cuts at:      0     513     870 
   Size:        513     357
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0      10      42     870 
   Size:         10      32     828

  Fragments arranged by size: 

                 828      32      10
 
PstI C_TGCA'G

Cuts at:      0     155     870 
   Size:        155     715
 
SacI G_AGCT'C

Cuts at:      0     538     870 
   Size:        538     332
 
ScaI AGT'ACT

Cuts at:      0     277     870 
   Size:        277     593
 
SfcI C'TryA_G

Cuts at:      0     151     609     870 
   Size:        151     458     261

  Fragments arranged by size: 

                 458     261     151
 
SspI AAT'ATT

Cuts at:      0     504     870 
   Size:        504     366
 
StuI AGG'CCT

Cuts at:      0     860     870 
   Size:        860      10
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     233     870 
   Size:        233     637
 
TatI w'GTAC_w

Cuts at:      0      26      35     275     870 
   Size:         26       9     240     595

  Fragments arranged by size: 

                 595     240      26       9
 
XbaI T'CTAG_A

Cuts at:      0     627     870 
   Size:        627     243
 

 Enzymes that do cut:

     AccI    AlwNI     ApoI     AvaI    BanII     BbsI    BciVI     BglI
     BmrI     BplI     BpmI     BsaI    BseRI    BseYI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BsrFI     BtgI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    HaeII    Hin4I     MmeI   MspA1I     NaeI
   NgoMIV    PflMI     PfoI    PpuMI     PsrI     PstI     SacI     ScaI
     SfcI     SspI     StuI    TaqII     TatI     XbaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmtI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI
    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StyI     SwaI     TstI
  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI
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