Restriction map for pF1KB8935
##################################
# NO CUT ENZYMES (  160 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwNI       Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BamHI       BanII       BbeI        BbrPI       BbsI        
BbvCI       BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        Bsp1407I    
BspEI       BspHI       BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstEII      
BstPI       BstUI       BstZ17I     CelII       CfoI        
Cfr10I      CpoI        DraI        DraIII      DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRI       EcoT14I     EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
HphI        KasI        KpnI        KspI        MaeII       
MamI        MluI        MroI        MslI        MspI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        PacI        PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SbfI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        StyI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AcsI          561  AAATTT               [GA]^AATT[CT]
AflIII        733  ACATGT               A^C[GA][CT]GT
AlwI          454  GGATC                GGATC(4/5)
AocI          250  CCTGAGG              CC^T[ACGT]AGG
ApoI          561  AAATTT               [GA]^AATT[CT]
BalI          698  TGGCCA               TGG^CCA
BanI          345  GGCACC               G^G[CT][GA]CC
BcgI          229  GCAATTACATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI          344  GGGCAC               G[AGT]GC[ACT]^C
BsgI          367  GTGCAG               GTGCAG(16/14)
BsiYI         335  CCTTTCCTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          335  CCTTTCCTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         712  GGGAC                GGGAC(10/14)
Bsp1286I      344  GGGCAC               G[AGT]GC[ACT]^C
BspDI         836  ATCGAT               AT^CGAT
BspLU11I      733  ACATGT               A^CATGT
BspMI         225  ACCTGC               ACCTGC(4/8)
BstBI         404  TTCGAA               TT^CGAA
BstXI          26  CCAAGAAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         454  GGATCT               [GA]^GATC[CT]
Bsu36I        250  CCTGAGG              CC^T[ACGT]AGG
ClaI          836  ATCGAT               AT^CGAT
DpnII         455  GATC                 ^GATC
DraII         712  GGGACCC              [GA]G^G[ACGT]CC[CT]
DrdI            7  GACGAAGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          698  TGGCCA               [CT]^GGCC[GA]
EarI          401  CTCTTC               CTCTTC(1/4)
Eco81I        250  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      712  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRV         531  GATATC               GAT^ATC
Ksp632I       401  CTCTTC               CTCTTC(1/4)
MboI          455  GATC                 ^GATC
MfeI          428  CAATTG               C^AATTG
MflI          454  GGATCT               [GA]^GATC[CT]
MluNI         698  TGGCCA               TGG^CCA
MscI          698  TGGCCA               TGG^CCA
MspA1I        276  CAGCGG               C[AC]G^C[GT]G
MunI          428  CAATTG               C^AATTG
NdeII         455  GATC                 ^GATC
NspI          733  ACATGT               [GA]CATG^[CT]
NspV          404  TTCGAA               TT^CGAA
PaeR7I        314  CTCGAG               C^TCGAG
PpuMI         712  GGGACCC              [GA]G^G[AT]CC[CT]
SapI          400  GCTCTTC              GCTCTTC(1/4)
Sau3AI        455  GATC                 ^GATC
ScaI          268  AGTACT               AGT^ACT
SfuI          404  TTCGAA               TT^CGAA
SmlI          314  CTCGAG               C^T[CT][GA]AG
Tsp45I        802  GTGAC                ^GT[GC]AC
TspRI         800  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoI          314  CTCGAG               C^TCGAG
XhoII         454  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AciI           18  CCGC                 CCGC(-3/-1)
              278  GCGG                
AvaI          314  CTCGAG               C^[CT]CG[GA]G
              341  CTCGGG              
AvaII          15  GGTCC                G^G[AT]CC
              713  GGACC               
BbvI          397  GCAGC                GCAGC(8/12)
              394  GCTGC               
BfaI          611  CTAG                 C^TAG
              635  CTAG                
BsaJI          59  CCCTGG               C^C[ACGT][ACGT]GG
              340  CCTCGG              
BsmAI         304  GAGAC                GTCTC(1/5)
              150  GTCTC               
BsoBI         314  CTCGAG               C^[CT]CG[GA]G
              341  CTCGGG              
BsrDI         423  CATTGC               GCAATG(2/0)
              281  GCAATG              
BsrI          706  ACTGG                ACTGG(1/-1)
              799  CCAGT               
BssKI          60  CCTGG                ^CC[ACGT]GG
              164  CCTGG               
BstNI          60  CCTGG                CC^[AT]GG
              164  CCTGG               
Cac8I         110  GCAAGC               GC[ACGT]^[ACGT]GC
              390  GCTTGC              
DdeI          194  CTGAG                C^T[ACGT]AG
              251  CTGAG               
EcoRII         60  CCTGG                ^CC[AT]GG
              164  CCTGG               
HaeIII        185  GGCC                 GG^CC
              699  GGCC                
HinfI          83  GAATC                G^A[ACGT]TC
              361  GATTC               
MaeI          611  CTAG                 C^TAG
              635  CTAG                
MaeIII        133  GTTAC                ^GT[ACGT]AC
              802  GTGAC               
MvaI           60  CCTGG                CC^[AT]GG
              164  CCTGG               
MwoI          281  GCAATGTAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              427  GCAATTGGAGC         
NlaIII        501  CATG                 CATG^
              734  CATG                
PstI           70  CTGCAG               CTGCA^G
              395  CTGCAG              
ScrFI          60  CCTGG                CC^[ACGT]GG
              164  CCTGG               
SfcI           70  CTGCAG               C^T[GA][CT]AG
              395  CTGCAG              
SspI           46  AATATT               AAT^ATT
              589  AATATT              
TfiI           83  GAATC                G^A[AT]TC
              361  GATTC               
TseI          394  GCTGC                G^C[AT]GC
              397  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AfaI        AluI        BstF5I      Cfr13I      Fnu4HI      
FokI        ItaI        MboII       MnlI        MseI        
NlaIV       RsaI        Sau96I      SfaNI       TaqI        
Tru9I       Tsp509I     TthHB8I     
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