Restriction map for pF1KB8932
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BalI        
BamHI       BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfrI        BlnI        BpuAI       
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BsgI        BsiHKAI     BsmBI       BsmI        BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    EarI        Eco47III    
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HinfI       HpaI        KpnI        
Ksp632I     MaeII       MamI        MboII       MfeI        
MluNI       MroI        MscI        MseI        MslI        
MunI        NaeI        NdeI        NgoMIV      NheI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacI        
SalI        SapI        SbfI        SexAI       SfaNI       
SfcI        SfiI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        TaqI        TfiI        
Tru9I       Tsp509I     Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI          369  GTCTAC               GT^[AC][GT]AC
AflIII        383  ACGCGT               A^C[GA][CT]GT
ApaI          292  GGGCCC               GGGCC^C
Asp700I       486  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          767  CCCGAG               C^[CT]CG[GA]G
AvaII          45  GGACC                G^G[AT]CC
BfaI          475  CTAG                 C^TAG
BglII         572  AGATCT               A^GATCT
BlpI          262  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      262  GCTCAGC              GC^T[ACGT]AGC
BsiWI         200  CGTACG               C^GTACG
BsmAI         561  GAGAC                GTCTC(1/5)
BsmFI         634  GGGAC                GGGAC(10/14)
BsoBI         767  CCCGAG               C^[CT]CG[GA]G
Bsp1407I      107  TGTACA               T^GTACA
BsrGI         107  TGTACA               T^GTACA
BsrI          396  CCAGT                ACTGG(1/-1)
BstF5I        362  GGATG                GGATG(2/0)
BstYI         572  AGATCT               [GA]^GATC[CT]
CelII         262  GCTCAGC              GC^T[ACGT]AGC
DpnII         573  GATC                 ^GATC
EcoT14I       358  CCATGG               C^C[AT][AT]GG
FokI          362  GGATG                GGATG(9/13)
HphI          246  TCACC                GGTGA(8/7)
KspI          230  CCGCGG               CCGC^GG
MaeI          475  CTAG                 C^TAG
MaeIII        685  GTGAC                ^GT[ACGT]AC
MboI          573  GATC                 ^GATC
MflI          572  AGATCT               [GA]^GATC[CT]
MluI          383  ACGCGT               A^CGCGT
NcoI          358  CCATGG               C^CATGG
NdeII         573  GATC                 ^GATC
NotI          831  GCGGCCGC             GC^GGCCGC
PpuMI          44  AGGACCC              [GA]G^G[AT]CC[CT]
PshAI         636  GACAGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII         230  CCGCGG               CCGC^GG
Sau3AI        573  GATC                 ^GATC
ScaI           17  AGTACT               AGT^ACT
SspBI         107  TGTACA               T^GTACA
StyI          358  CCATGG               C^C[AT][AT]GG
Tsp45I        685  GTGAC                ^GT[GC]AC
TspRI         397  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoII         572  AGATCT               [GA]^GATC[CT]
XmnI          486  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

BanII         292  GGGCCC               G[GA]GC[CT]^C
              331  GAGCCC              
BglI          285  GCCCGCCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              825  GCCTCGGCGGC         
BsiEI         517  CGGCCG               CG[GA][CT]^CG
              832  CGGCCG              
BssHII        164  GCGCGC               G^CGCGC
              462  GCGCGC              
DraII          44  AGGACCC              [GA]G^G[ACGT]CC[CT]
              292  GGGCCCT             
DsaI          230  CCGCGG               C^C[GA][CT]GG
              358  CCATGG              
EagI          517  CGGCCG               C^GGCCG
              832  CGGCCG              
EclXI         517  CGGCCG               C^GGCCG
              832  CGGCCG              
Eco52I        517  CGGCCG               C^GGCCG
              832  CGGCCG              
EcoO109I       44  AGGACCC              [GA]G^G[ACGT]CC[CT]
              292  GGGCCCT             


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
BanI        BbeI        BbvI        BcnI        BmyI        
BpmI        BsaHI       BsaJI       BseRI       BsiYI       
BslI        Bsp1286I    BssKI       BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        EaeI        
EcoRII      FauI        Fnu4HI      HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HpaII       ItaI        KasI        MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NarI        
NciI        NlaIII      NlaIV       PvuII       RsaI        
Sau96I      ScrFI       SfoI        TseI        
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