Restriction map for pF1KB8925
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       AccI        AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        AspEI       AspHI       AspI        AviII       
AvrII       BamHI       BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsiHKAI     BsiWI       BsmBI       Bsp1407I    
BspDI       BspHI       BspLU11I    BsrDI       BsrGI       
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     CelII       ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        Eam1105I    
Eco47III    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KspI        MaeI        MaeII       
MamI        MfeI        MluI        MseI        MslI        
MunI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SexAI       SfaNI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        TfiI        Tru9I       Tsp509I     Tth111I     
TthHB8I     XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

Acc65I        452  GGTACC               G^GTACC
AccIII         93  TCCGGA               T^CCGGA
AocI          286  CCTCAGG              CC^T[ACGT]AGG
ApaI          112  GGGCCC               GGGCC^C
Asp700I       186  GAACCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       452  GGTACC               G^GTACC
BglII         518  AGATCT               A^GATCT
BpmI          427  CTGGAG               CTGGAG(16/14)
BsaI          349  GAGACC               GGTCTC(1/5)
BseAI          93  TCCGGA               T^CCGGA
BseRI          70  GAGGAG               GAGGAG(10/8)
BsiEI         320  CGGCCG               CG[GA][CT]^CG
BsmAI         349  GAGAC                GTCTC(1/5)
BsmI          692  GCATTC               GAATGC(1/-1)
BspEI          93  TCCGGA               T^CCGGA
BsrBI         430  GAGCGG               CCGCTC(-3/-3)
BsrFI         373  GCCGGC               [GA]^CCGG[CT]
BstF5I        492  CATCC                GGATG(2/0)
BstYI         518  AGATCT               [GA]^GATC[CT]
Bsu36I        286  CCTCAGG              CC^T[ACGT]AGG
Cfr10I        373  GCCGGC               [GA]^CCGG[CT]
DpnII         519  GATC                 ^GATC
EagI          320  CGGCCG               C^GGCCG
EarI          100  GAAGAG               CTCTTC(1/4)
EclXI         320  CGGCCG               C^GGCCG
Eco52I        320  CGGCCG               C^GGCCG
Eco81I        286  CCTCAGG              CC^T[ACGT]AGG
EcoNI         792  CCTGGTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       407  CCAAGG               C^C[AT][AT]GG
FokI          492  CATCC                GGATG(9/13)
KpnI          452  GGTACC               GGTAC^C
Ksp632I       100  GAAGAG               CTCTTC(1/4)
MboI          519  GATC                 ^GATC
MflI          518  AGATCT               [GA]^GATC[CT]
MroI           93  TCCGGA               T^CCGGA
NaeI          373  GCCGGC               GCC^GGC
NdeII         519  GATC                 ^GATC
NgoMIV        373  GCCGGC               G^CCGGC
NspI          731  ACATGC               [GA]CATG^[CT]
PflMI         660  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         662  AGGACCT              [GA]G^G[AT]CC[CT]
PstI          724  CTGCAG               CTGCA^G
Sau3AI        519  GATC                 ^GATC
ScaI          251  AGTACT               AGT^ACT
SmlI          294  CTCAAG               C^T[CT][GA]AG
StyI          407  CCAAGG               C^C[AT][AT]GG
TspRI         808  CAGTG                CA[GC]TG[ACGT][ACGT]^
Van91I        660  CCAGGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         518  AGATCT               [GA]^GATC[CT]
XmnI          186  GAACCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

BalI          230  TGGCCA               TGG^CCA
              482  TGGCCA              
BglI          124  GCCAAGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              138  GCCGCTGGGGC         
BsaWI          93  TCCGGA               [AT]^CCGG[AT]
              333  ACCGGA              
BsgI          259  CTGCAC               GTGCAG(16/14)
              166  GTGCAG              
BspMI          50  ACCTGC               ACCTGC(4/8)
              510  GCAGGT              
BsrI          747  CCAGT                ACTGG(1/-1)
              771  CCAGT               
BssHII        552  GCGCGC               G^CGCGC
              713  GCGCGC              
DdeI           45  CTTAG                C^T[ACGT]AG
              287  CTCAG               
FauI          362  GCGGG                CCCGC(4/6)
              688  GCGGG               
HinfI         292  GACTC                G^A[ACGT]TC
              328  GAGTC               
HphI          579  GGTGA                GGTGA(8/7)
              331  TCACC               
MboII         100  GAAGA                GAAGA(8/7)
              183  GAAGA               
MluNI         230  TGGCCA               TGG^CCA
              482  TGGCCA              
MscI          230  TGGCCA               TGG^CCA
              482  TGGCCA              
MspA1I        139  CCGCTG               C[AC]G^C[GT]G
              448  CAGCGG              
NlaIII        641  CATG                 CATG^
              732  CATG                
PleI          292  GACTC                GAGTC(4/5)
              328  GAGTC               
SfcI          708  CTACAG               C^T[GA][CT]AG
              724  CTGCAG              
SmaI          354  CCCGGG               CCC^GGG
              557  CCCGGG              
XmaCI         354  CCCGGG               C^CCGGG
              557  CCCGGG              
XmaI          354  CCCGGG               C^CCGGG
              557  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       AvaI        AvaII       BanI        BanII       
BbeI        BbvI        BcnI        BmyI        BsaHI       
BsaJI       BsiYI       BslI        BsmFI       BsoBI       
Bsp1286I    BssKI       BstAPI      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DraII       EaeI        
EcoO109I    EcoRII      Fnu4HI      HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HpaII       ItaI        KasI        MaeIII      MnlI        
MspI        MvaI        MvnI        MwoI        NarI        
NciI        NlaIV       RsaI        Sau96I      ScrFI       
SfoI        TseI        Tsp45I      
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com