Restriction map for pF1KB8919
 (Linear) (Six-base) MAPSORT of: KIBB8919.seq  Check: 5740  from: 1  to: 792



KIBB8919.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AccI GT'mk_AC

Cuts at:      0     404     792 
   Size:        404     388
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     749     792 
   Size:        749      43
 
AfeI AGC'GCT

Cuts at:      0     472     792 
   Size:        472     320
 
AlwNI CAG_nnn'CTG

Cuts at:      0     153     792 
   Size:        153     639
 
ApaI G_GGCC'C

Cuts at:      0     224     792 
   Size:        224     568
 
ApoI r'AATT_y

Cuts at:      0      46     422     792 
   Size:         46     376     370

  Fragments arranged by size: 

                 376     370      46
 
AvaI C'yCGr_G

Cuts at:      0     271     474     717     792 
   Size:        271     203     243      75

  Fragments arranged by size: 

                 271     243     203      75
 
BanI G'GyrC_C

Cuts at:      0     300     313     792 
   Size:        300      13     479

  Fragments arranged by size: 

                 479     300      13
 
BanII G_rGCy'C

Cuts at:      0      13     224     792 
   Size:         13     211     568

  Fragments arranged by size: 

                 568     211      13
 
BbeI G_GCGC'C

Cuts at:      0     304     317     792 
   Size:        304      13     475

  Fragments arranged by size: 

                 475     304      13
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     737     792 
   Size:        737      55
 
BclI T'GATC_A

Cuts at:      0       2     792 
   Size:          2     790
 
Bme1580I G_kGCm'C

Cuts at:      0     224     792 
   Size:        224     568
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     199     360     792 
   Size:        199     161     432

  Fragments arranged by size: 

                 432     199     161
 
Bpu10I CC'TnA_GC

Cuts at:      0     188     792 
   Size:        188     604
 
BsaBI GATnn'nnATC

Cuts at:      0     562     601     792 
   Size:        562      39     191

  Fragments arranged by size: 

                 562     191      39
 
BsaHI Gr'CG_yC

Cuts at:      0     301     314     792 
   Size:        301      13     478

  Fragments arranged by size: 

                 478     301      13
 
BsaWI w'CCGG_w

Cuts at:      0      99     792 
   Size:         99     693
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     358     388     792 
   Size:        358      30     404

  Fragments arranged by size: 

                 404     358      30
 
BseYI C'CCAG_C

Cuts at:      0     333     694     792 
   Size:        333     361      98

  Fragments arranged by size: 

                 361     333      98
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     189     792 
   Size:        189     603
 
BsiEI CG_ry'CG

Cuts at:      0     114     683     792 
   Size:        114     569     109

  Fragments arranged by size: 

                 569     114     109
 
BsiHKAI G_wGCw'C

Cuts at:      0      13     792 
   Size:         13     779
 
Bsp1286I G_dGCh'C

Cuts at:      0      13     224     792 
   Size:         13     211     568

  Fragments arranged by size: 

                 568     211      13
 
BspEI T'CCGG_A

Cuts at:      0      99     792 
   Size:         99     693
 
BspHI T'CATG_A

Cuts at:      0       5     792 
   Size:          5     787
 
BsrFI r'CCGG_y

Cuts at:      0     246     297     682     792 
   Size:        246      51     385     110

  Fragments arranged by size: 

                 385     246     110      51
 
BtgI C'CryG_G

Cuts at:      0     240     409     792 
   Size:        240     169     383

  Fragments arranged by size: 

                 383     240     169
 
ClaI AT'CG_AT

Cuts at:      0      38     792 
   Size:         38     754
 
EaeI y'GGCC_r

Cuts at:      0     111     680     792 
   Size:        111     569     112

  Fragments arranged by size: 

                 569     112     111
 
EagI C'GGCC_G

Cuts at:      0     111     680     792 
   Size:        111     569     112

  Fragments arranged by size: 

                 569     112     111
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     309     792 
   Size:        309     483
 
EcoICRI GAG'CTC

Cuts at:      0      11     792 
   Size:         11     781
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     199     360     749     792 
   Size:        199     161     389      43

  Fragments arranged by size: 

                 389     199     161      43
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     308     792 
   Size:        308     484
 
EcoO109I rG'GnC_Cy

Cuts at:      0     220     792 
   Size:        220     572
 
FseI GG_CCGG'CC

Cuts at:      0     250     792 
   Size:        250     542
 
HaeII r_GCGC'y

Cuts at:      0     195     304     317     383     474     689     749     792 
   Size:        195     109      13      66      91     215      60      43

  Fragments arranged by size: 

                 215     195     109      91      66      60      43      13
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     358     390     792 
   Size:        358      32     402

  Fragments arranged by size: 

                 402     358      32
 
KasI G'GCGC_C

Cuts at:      0     300     313     792 
   Size:        300      13     479

  Fragments arranged by size: 

                 479     300      13
 
NaeI GCC'GGC

Cuts at:      0     248     299     684     792 
   Size:        248      51     385     108

  Fragments arranged by size: 

                 385     248     108      51
 
NarI GG'CG_CC

Cuts at:      0     301     314     792 
   Size:        301      13     478

  Fragments arranged by size: 

                 478     301      13
 
NcoI C'CATG_G

Cuts at:      0     409     792 
   Size:        409     383
 
NgoMIV G'CCGG_C

Cuts at:      0     246     297     682     792 
   Size:        246      51     385     110

  Fragments arranged by size: 

                 385     246     110      51
 
NruI TCG'CGA

Cuts at:      0     234     792 
   Size:        234     558
 
PfoI T'CCnGG_A

Cuts at:      0     503     792 
   Size:        503     289
 
PsiI TTA'TAA

Cuts at:      0     452     792 
   Size:        452     340
 
PspOMI G'GGCC_C

Cuts at:      0     220     792 
   Size:        220     572
 
PspXI vC'TCGA_Gb

Cuts at:      0     474     792 
   Size:        474     318
 
PstI C_TGCA'G

Cuts at:      0     352     792 
   Size:        352     440
 
SacI G_AGCT'C

Cuts at:      0      13     792 
   Size:         13     779
 
SexAI A'CCwGG_T

Cuts at:      0     784     792 
   Size:        784       8
 
SfcI C'TryA_G

Cuts at:      0     348     792 
   Size:        348     444
 
SfoI GGC'GCC

Cuts at:      0     302     315     792 
   Size:        302      13     477

  Fragments arranged by size: 

                 477     302      13
 
SmaI CCC'GGG

Cuts at:      0     719     792 
   Size:        719      73
 
SmlI C'TyrA_G

Cuts at:      0     474     792 
   Size:        474     318
 
SspI AAT'ATT

Cuts at:      0      67     106     792 
   Size:         67      39     686

  Fragments arranged by size: 

                 686      67      39
 
StyI C'CwwG_G

Cuts at:      0     409     792 
   Size:        409     383
 
XhoI C'TCGA_G

Cuts at:      0     474     792 
   Size:        474     318
 
XmaI C'CCGG_G

Cuts at:      0     717     792 
   Size:        717      75
 

 Enzymes that do cut:

     AccI     AcuI     AfeI    AlwNI     ApaI     ApoI     AvaI     BanI
    BanII     BbeI     BbsI     BclI Bme1580I     BpmI   Bpu10I    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspHI    BsrFI     BtgI     ClaI     EaeI     EagI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I     FseI    HaeII    Hin4I     KasI
     NaeI     NarI     NcoI   NgoMIV     NruI     PfoI     PsiI   PspOMI
    PspXI     PstI     SacI    SexAI     SfcI     SfoI     SmaI     SmlI
     SspI     StyI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI
    BseRI    BsiWI     BsmI    BsmBI    BspMI    BsrBI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI    CspCI     DraI   DraIII     DrdI     EarI
    EcoRI    EcoRV     FalI     FspI    FspAI   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NdeI
     NheI     NotI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsrI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmnI     ZraI
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