Restriction map for pF1KB8917
##################################
# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AlwI        AocI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BamHI       
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaBI       BsaI        BsaWI       BseAI       BsgI        
BsiHKAI     BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BsrDI       BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstYI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DpnII       
DraI        DrdI        Eam1105I    Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        KspI        MamI        
MboI        MfeI        MflI        MluI        MroI        
MunI        NcoI        NdeI        NdeII       NheI        
NotI        NruI        NsiI        NspV        PacI        
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      SbfI        
ScaI        SexAI       SfaNI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tsp509I     Tth111I     XbaI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AflIII        422  ACATGT               A^C[GA][CT]GT
Aor51HI       584  AGCGCT               AGC^GCT
ApaI           13  GGGCCC               GGGCC^C
BalI          548  TGGCCA               TGG^CCA
BbsI           61  GAAGAC               GAAGAC(2/6)
BfaI           70  CTAG                 C^TAG
BglI           88  GCCACCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          619  CTGGAG               CTGGAG(16/14)
BpuAI          61  GAAGAC               GAAGAC(2/6)
BsaAI          31  TACGTG               [CT]AC^GT[GA]
BseRI         514  GAGGAG               GAGGAG(10/8)
BsiWI         236  CGTACG               C^GTACG
BsmAI         310  GTCTC                GTCTC(1/5)
BsmBI         309  CGTCTC               CGTCTC(1/5)
BsmFI         656  GGGAC                GGGAC(10/14)
BspLU11I      422  ACATGT               A^CATGT
BspMI         702  GCAGGT               ACCTGC(4/8)
BssSI         573  CACGAG               CACGAG(-5/-1)
BstAPI        462  GCAGACACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         119  CCAGCGGTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI          212  CTCAG                C^T[ACGT]AG
DraII          12  GGGGCCC              [GA]G^G[ACGT]CC[CT]
DraIII        189  CACGTCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          329  CGGCCG               C^GGCCG
EarI          634  CTCTTC               CTCTTC(1/4)
EclXI         329  CGGCCG               C^GGCCG
Eco47III      584  AGCGCT               AGC^GCT
Eco52I        329  CGGCCG               C^GGCCG
EcoO109I       12  GGGGCCC              [GA]G^G[ACGT]CC[CT]
Ksp632I       634  CTCTTC               CTCTTC(1/4)
MaeI           70  CTAG                 C^TAG
MluNI         548  TGGCCA               TGG^CCA
MscI          548  TGGCCA               TGG^CCA
MseI          649  TTAA                 T^TAA
MslI           52  CACGACAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I        289  CTCGAG               C^TCGAG
PflMI         606  CCAGACGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI           37  GACTC                GAGTC(4/5)
Tru9I         649  TTAA                 T^TAA
Tsp45I        341  GTCAC                ^GT[GC]AC
Van91I        606  CCAGACGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          536  CCAGCAACCCCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          289  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AlwNI         463  CAGACACTG            CAG[ACGT][ACGT][ACGT]^CTG
              607  CAGACGCTG           
AvaII         531  GGACC                G^G[AT]CC
              657  GGACC               
BanII          13  GGGCCC               G[GA]GC[CT]^C
               95  GGGCTC              
BsiEI         329  CGGCCG               CG[GA][CT]^CG
              450  CGACCG              
BsrBI         567  CCGCTC               CCGCTC(-3/-3)
              697  GAGCGG              
BsrFI         174  GCCGGC               [GA]^CCGG[CT]
              496  GCCGGC              
BsrI          554  ACTGG                ACTGG(1/-1)
              618  ACTGG               
BstF5I        100  CATCC                GGATG(2/0)
              731  GGATG               
Cfr10I        174  GCCGGC               [GA]^CCGG[CT]
              496  GCCGGC              
DsaI          221  CCGTGG               C^C[GA][CT]GG
              545  CCGTGG              
FokI          100  CATCC                GGATG(9/13)
              731  GGATG               
HphI          231  TCACC                GGTGA(8/7)
              692  TCACC               
MaeII          32  ACGT                 A^CGT
              190  ACGT                
MaeIII         23  GTAAC                ^GT[ACGT]AC
              341  GTCAC               
MspA1I        120  CAGCGG               C[AC]G^C[GT]G
              295  CCGCTG              
NaeI          174  GCCGGC               GCC^GGC
              496  GCCGGC              
NgoMIV        174  GCCGGC               G^CCGGC
              496  GCCGGC              
NlaIII        276  CATG                 CATG^
              423  CATG                
NspI          275  ACATGC               [GA]CATG^[CT]
              422  ACATGT              
SfcI          138  CTGTAG               C^T[GA][CT]AG
              156  CTGTAG              
SmlI          289  CTCGAG               C^T[CT][GA]AG
              352  CTCAAG              
TaqI          290  TCGA                 T^CGA
              726  TCGA                
TfiI          558  GATTC                G^A[AT]TC
              723  GAATC               
TspRI         179  CAGTG                CA[GC]TG[ACGT][ACGT]^
              467  CACTG               
TthHB8I       290  TCGA                 T^CGA
              726  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AvaI        BanI        BbeI        BbvI        BcnI        
BmyI        BsaHI       BsaJI       BsiYI       BslI        
BsoBI       Bsp1286I    BssKI       BstNI       BstUI       
Cac8I       CfoI        Cfr13I      EaeI        EcoRII      
FauI        Fnu4HI      HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       ItaI        KasI        MboII       MnlI        
MspI        MvaI        MvnI        MwoI        NarI        
NciI        NlaIV       RsaI        Sau96I      ScrFI       
SfoI        SmaI        TseI        XmaCI       XmaI        
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