Restriction map for pF1KB8917
 (Linear) (Six-base) MAPSORT of: KIBB8917.seq  Check: 1060  from: 1  to: 780



KIBB8917.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AfeI AGC'GCT

Cuts at:      0     586     780 
   Size:        586     194
 
AflIII A'CryG_T

Cuts at:      0     422     780 
   Size:        422     358
 
AlwNI CAG_nnn'CTG

Cuts at:      0     468     612     780 
   Size:        468     144     168

  Fragments arranged by size: 

                 468     168     144
 
ApaI G_GGCC'C

Cuts at:      0      17     780 
   Size:         17     763
 
AvaI C'yCGr_G

Cuts at:      0     289     370     436     478     677     780 
   Size:        289      81      66      42     199     103

  Fragments arranged by size: 

                 289     199     103      81      66      42
 
BanI G'GyrC_C

Cuts at:      0     129     198     259     304     374     780 
   Size:        129      69      61      45      70     406

  Fragments arranged by size: 

                 406     129      70      69      61      45
 
BanII G_rGCy'C

Cuts at:      0      17      99     780 
   Size:         17      82     681

  Fragments arranged by size: 

                 681      82      17
 
BbeI G_GCGC'C

Cuts at:      0     202     263     308     378     780 
   Size:        202      61      45      70     402

  Fragments arranged by size: 

                 402     202      70      61      45
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      68     780 
   Size:         68     712
 
BglI GCCn_nnn'nGGC

Cuts at:      0      94     780 
   Size:         94     686
 
Bme1580I G_kGCm'C

Cuts at:      0      17     209     780 
   Size:         17     192     571

  Fragments arranged by size: 

                 571     192      17
 
BmgBI CAC'GTC

Cuts at:      0     191     780 
   Size:        191     589
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     640     780 
   Size:        640     140
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     780 
   Size:        337     443
 
BsaAI yAC'GTr

Cuts at:      0      33     780 
   Size:         33     747
 
BsaHI Gr'CG_yC

Cuts at:      0     199     260     305     362     375     780 
   Size:        199      61      45      57      13     405

  Fragments arranged by size: 

                 405     199      61      57      45      13
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     553     583     613     643     780 
   Size:        553      30      30      30     137

  Fragments arranged by size: 

                 553     137      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     529     780 
   Size:        529     251
 
BseYI C'CCAG_C

Cuts at:      0     118     245     319     535     780 
   Size:        118     127      74     216     245

  Fragments arranged by size: 

                 245     216     127     118      74
 
BsiEI CG_ry'CG

Cuts at:      0     332     453     780 
   Size:        332     121     327

  Fragments arranged by size: 

                 332     327     121
 
BsiWI C'GTAC_G

Cuts at:      0     236     780 
   Size:        236     544
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     315     780 
   Size:        315     465
 
Bsp1286I G_dGCh'C

Cuts at:      0      17      99     209     780 
   Size:         17      82     110     571

  Fragments arranged by size: 

                 571     110      82      17
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     693     780 
   Size:        693      87
 
BsrBI CCG'CTC

Cuts at:      0     569     699     780 
   Size:        569     130      81

  Fragments arranged by size: 

                 569     130      81
 
BsrFI r'CCGG_y

Cuts at:      0     174     496     780 
   Size:        174     322     284

  Fragments arranged by size: 

                 322     284     174
 
BssSI C'ACGA_G

Cuts at:      0     573     780 
   Size:        573     207
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     468     780 
   Size:        468     312
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     126     780 
   Size:        126     654
 
BtgI C'CryG_G

Cuts at:      0     221     545     780 
   Size:        221     324     235

  Fragments arranged by size: 

                 324     235     221
 
BtsI GCAGTG_nn'

Cuts at:      0     185     466     780 
   Size:        185     281     314

  Fragments arranged by size: 

                 314     281     185
 
DraIII CAC_nnn'GTG

Cuts at:      0     194     780 
   Size:        194     586
 
EaeI y'GGCC_r

Cuts at:      0      86     329     548     780 
   Size:         86     243     219     232

  Fragments arranged by size: 

                 243     232     219      86
 
EagI C'GGCC_G

Cuts at:      0     329     780 
   Size:        329     451
 
EarI CTCTTCn'nnn_

Cuts at:      0     640     780 
   Size:        640     140
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     499     780 
   Size:        499     281
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     640     780 
   Size:        640     140
 
EcoO109I rG'GnC_Cy

Cuts at:      0      13     780 
   Size:         13     767
 
HaeII r_GCGC'y

Cuts at:      0     202     263     308     378     588     780 
   Size:        202      61      45      70     210     192

  Fragments arranged by size: 

                 210     202     192      70      61      45
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     136     168     283     315     780 
   Size:        136      32     115      32     465

  Fragments arranged by size: 

                 465     136     115      32      32
 
KasI G'GCGC_C

Cuts at:      0     198     259     304     374     780 
   Size:        198      61      45      70     406

  Fragments arranged by size: 

                 406     198      70      61      45
 
MscI TGG'CCA

Cuts at:      0     550     780 
   Size:        550     230
 
MslI CAynn'nnrTG

Cuts at:      0      56     780 
   Size:         56     724
 
MspA1I CmG'CkG

Cuts at:      0     122     297     780 
   Size:        122     175     483

  Fragments arranged by size: 

                 483     175     122
 
NaeI GCC'GGC

Cuts at:      0     176     498     780 
   Size:        176     322     282

  Fragments arranged by size: 

                 322     282     176
 
NarI GG'CG_CC

Cuts at:      0     199     260     305     375     780 
   Size:        199      61      45      70     405

  Fragments arranged by size: 

                 405     199      70      61      45
 
NgoMIV G'CCGG_C

Cuts at:      0     174     496     780 
   Size:        174     322     284

  Fragments arranged by size: 

                 322     284     174
 
NspI r_CATG'y

Cuts at:      0     279     426     780 
   Size:        279     147     354

  Fragments arranged by size: 

                 354     279     147
 
PasI CC'CwG_GG

Cuts at:      0     394     780 
   Size:        394     386
 
PciI A'CATG_T

Cuts at:      0     422     780 
   Size:        422     358
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     612     780 
   Size:        612     168
 
PspOMI G'GGCC_C

Cuts at:      0      13     780 
   Size:         13     767
 
PspXI vC'TCGA_Gb

Cuts at:      0     289     780 
   Size:        289     491
 
SfcI C'TryA_G

Cuts at:      0     138     156     780 
   Size:        138      18     624

  Fragments arranged by size: 

                 624     138      18
 
SfoI GGC'GCC

Cuts at:      0     200     261     306     376     780 
   Size:        200      61      45      70     404

  Fragments arranged by size: 

                 404     200      70      61      45
 
SmaI CCC'GGG

Cuts at:      0     372     438     679     780 
   Size:        372      66     241     101

  Fragments arranged by size: 

                 372     241     101      66
 
SmlI C'TyrA_G

Cuts at:      0     289     352     780 
   Size:        289      63     428

  Fragments arranged by size: 

                 428     289      63
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     553     585     780 
   Size:        553      32     195

  Fragments arranged by size: 

                 553     195      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     543     780 
   Size:        543     237
 
XhoI C'TCGA_G

Cuts at:      0     289     780 
   Size:        289     491
 
XmaI C'CCGG_G

Cuts at:      0     370     436     677     780 
   Size:        370      66     241     103

  Fragments arranged by size: 

                 370     241     103      66
 

 Enzymes that do cut:

     AfeI   AflIII    AlwNI     ApaI     AvaI     BanI    BanII     BbeI
     BbsI     BglI Bme1580I    BmgBI     BpmI    BpuEI    BsaAI    BsaHI
    BsaXI    BseRI    BseYI    BsiEI    BsiWI    BsmBI Bsp1286I    BspMI
    BsrBI    BsrFI    BssSI   BstAPI    BstXI     BtgI     BtsI   DraIII
     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I    HaeII    Hin4I
     KasI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI
     PasI     PciI    PflMI   PspOMI    PspXI     SfcI     SfoI     SmaI
     SmlI     TstI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI     BmrI     BmtI     BplI   Bpu10I     BsaI
    BsaBI    BsaWI     BsgI  BsiHKAI     BsmI    BspEI    BspHI    BsrDI
    BsrGI   BssHII    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI     DrdI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI  Tth111I     XbaI     XmnI     ZraI
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