Restriction map for pF1KB8901
 (Linear) (Six-base) MAPSORT of: KIBB8901.seq  Check: 9731  from: 1  to: 699



KIBB8901.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AccI GT'mk_AC

Cuts at:      0     464     699 
   Size:        464     235
 
Acc65I G'GTAC_C

Cuts at:      0     299     699 
   Size:        299     400
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     672     699 
   Size:        672      27
 
AfeI AGC'GCT

Cuts at:      0     266     699 
   Size:        266     433
 
AvaI C'yCGr_G

Cuts at:      0     230     699 
   Size:        230     469
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     283     316     557     590     699 
   Size:        283      33     241      33     109

  Fragments arranged by size: 

                 283     241     109      33      33
 
BamHI G'GATC_C

Cuts at:      0     542     699 
   Size:        542     157
 
BanI G'GyrC_C

Cuts at:      0     170     299     699 
   Size:        170     129     400

  Fragments arranged by size: 

                 400     170     129
 
BanII G_rGCy'C

Cuts at:      0     682     699 
   Size:        682      17
 
BbvCI CC'TCA_GC

Cuts at:      0     268     699 
   Size:        268     431
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      55     558     699 
   Size:         55     503     141

  Fragments arranged by size: 

                 503     141      55
 
BglI GCCn_nnn'nGGC

Cuts at:      0     614     699 
   Size:        614      85
 
Bme1580I G_kGCm'C

Cuts at:      0     447     699 
   Size:        447     252
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     137     417     699 
   Size:        137     280     282

  Fragments arranged by size: 

                 282     280     137
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      90     699 
   Size:         90     609
 
Bpu10I CC'TnA_GC

Cuts at:      0     268     699 
   Size:        268     431
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     130     699 
   Size:        130     569
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      22      52     699 
   Size:         22      30     647

  Fragments arranged by size: 

                 647      30      22
 
BseYI C'CCAG_C

Cuts at:      0     329     560     675     699 
   Size:        329     231     115      24

  Fragments arranged by size: 

                 329     231     115      24
 
BsmI GAATG_Cn'

Cuts at:      0     310     699 
   Size:        310     389
 
Bsp1286I G_dGCh'C

Cuts at:      0     447     682     699 
   Size:        447     235      17

  Fragments arranged by size: 

                 447     235      17
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     468     699 
   Size:        468     231
 
BsrFI r'CCGG_y

Cuts at:      0     302     661     699 
   Size:        302     359      38

  Fragments arranged by size: 

                 359     302      38
 
BstYI r'GATC_y

Cuts at:      0     542     699 
   Size:        542     157
 
BtgI C'CryG_G

Cuts at:      0     188     245     699 
   Size:        188      57     454

  Fragments arranged by size: 

                 454     188      57
 
EaeI y'GGCC_r

Cuts at:      0     207     509     659     699 
   Size:        207     302     150      40

  Fragments arranged by size: 

                 302     207     150      40
 
EarI CTCTTCn'nnn_

Cuts at:      0      86     394     691     699 
   Size:         86     308     297       8

  Fragments arranged by size: 

                 308     297      86       8
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      90     672     699 
   Size:         90     582      27

  Fragments arranged by size: 

                 582      90      27
 
EcoO109I rG'GnC_Cy

Cuts at:      0     108     699 
   Size:        108     591
 
HaeII r_GCGC'y

Cuts at:      0     268     699 
   Size:        268     431
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      22      54     310     342     374     699 
   Size:         22      32     256      32      32     325

  Fragments arranged by size: 

                 325     256      32      32      32      22
 
HincII GTy'rAC

Cuts at:      0      30     699 
   Size:         30     669
 
HindIII A'AGCT_T

Cuts at:      0      46     699 
   Size:         46     653
 
KpnI G_GTAC'C

Cuts at:      0     303     699 
   Size:        303     396
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     472     673     699 
   Size:        472     201      26

  Fragments arranged by size: 

                 472     201      26
 
MscI TGG'CCA

Cuts at:      0     209     511     699 
   Size:        209     302     188

  Fragments arranged by size: 

                 302     209     188
 
MslI CAynn'nnrTG

Cuts at:      0     655     699 
   Size:        655      44
 
MspA1I CmG'CkG

Cuts at:      0      68     190     635     699 
   Size:         68     122     445      64

  Fragments arranged by size: 

                 445     122      68      64
 
NaeI GCC'GGC

Cuts at:      0     663     699 
   Size:        663      36
 
NcoI C'CATG_G

Cuts at:      0     245     699 
   Size:        245     454
 
NgoMIV G'CCGG_C

Cuts at:      0     661     699 
   Size:        661      38
 
PasI CC'CwG_GG

Cuts at:      0     346     699 
   Size:        346     353
 
PfoI T'CCnGG_A

Cuts at:      0     392     545     699 
   Size:        392     153     154

  Fragments arranged by size: 

                 392     154     153
 
PvuII CAG'CTG

Cuts at:      0      68     699 
   Size:         68     631
 
SacII CC_GC'GG

Cuts at:      0     191     699 
   Size:        191     508
 
SmaI CCC'GGG

Cuts at:      0     232     699 
   Size:        232     467
 
StyI C'CwwG_G

Cuts at:      0     245     538     699 
   Size:        245     293     161

  Fragments arranged by size: 

                 293     245     161
 
XmaI C'CCGG_G

Cuts at:      0     230     699 
   Size:        230     469
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI     AvaI     BaeI    BamHI     BanI
    BanII    BbvCI    BciVI     BglI Bme1580I     BmrI     BpmI   Bpu10I
     BsaI    BsaXI    BseYI     BsmI Bsp1286I    BspMI    BsrFI    BstYI
     BtgI     EaeI     EarI  Eco57MI EcoO109I    HaeII    Hin4I   HincII
  HindIII     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NcoI
   NgoMIV     PasI     PfoI    PvuII    SacII     SmaI     StyI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AclI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BbeI     BbsI     BcgI     BclI    BfrBI    BglII
     BlpI    BmgBI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI     HpaI     KasI     MfeI
     MluI     NarI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmnI     ZraI
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