Restriction map for pF1KB8895
##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BbvI        BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      CelII       
Cfr10I      Cfr13I      ClaI        CpoI        DraII       
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoT14I     EcoT22I     
FauI        FbaI        Fnu4HI      FseI        FspI        
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        HphI        ItaI        KasI        KpnI        
KspI        MaeII       MamI        MfeI        MluI        
MroI        MspA1I      MunI        MvnI        NaeI        
NarI        NcoI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SphI        Sse8387I    
SspBI       SspI        StyI        SwaI        TaqI        
TfiI        TseI        Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI          449  GTAGAC               GT^[AC][GT]AC
AflIII        503  ACATGT               A^C[GA][CT]GT
AluI           76  AGCT                 AG^CT
AseI           57  ATTAAT               AT^TAAT
AsnI           57  ATTAAT               AT^TAAT
Asp700I        55  GAATTAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          146  TGGCCA               TGG^CCA
BamHI         394  GGATCC               G^GATCC
BanII         177  GAGCCC               G[GA]GC[CT]^C
BcnI          180  CCCGG                CC^[GC]GG
BmyI          177  GAGCCC               G[AGT]GC[ACT]^C
Bsp1286I      177  GAGCCC               G[AGT]GC[ACT]^C
BspLU11I      503  ACATGT               A^CATGT
BstAPI        599  GCAAGCACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         432  CCAGG                CC^[AT]GG
Cac8I         599  GCAAGC               GC[ACGT]^[ACGT]GC
CfoI          117  GCGC                 GCG^C
DraI          355  TTTAAA               TTT^AAA
EaeI          146  TGGCCA               [CT]^GGCC[GA]
EarI          626  GAAGAG               CTCTTC(1/4)
EcoRII        432  CCAGG                ^CC[AT]GG
EcoRV          85  GATATC               GAT^ATC
HaeII         116  AGCGCC               [GA]GCGC^[CT]
HapII         181  CCGG                 C^CGG
HhaI          117  GCGC                 GCG^C
HinP1I        117  GCGC                 G^CGC
HinfI         547  GACTC                G^A[ACGT]TC
HpaII         181  CCGG                 C^CGG
Ksp632I       626  GAAGAG               CTCTTC(1/4)
MluNI         146  TGGCCA               TGG^CCA
MscI          146  TGGCCA               TGG^CCA
MslI           39  CATTTTAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          181  CCGG                 C^CGG
MvaI          432  CCAGG                CC^[AT]GG
MwoI          599  GCAAGCACTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI          180  CCCGG                CC^[GC]GG
NdeI          218  CATATG               CA^TATG
NspI          503  ACATGT               [GA]CATG^[CT]
PleI          547  GACTC                GAGTC(4/5)
PshBI          57  ATTAAT               AT^TAAT
PstI          606  CTGCAG               CTGCA^G
SfcI          606  CTGCAG               C^T[GA][CT]AG
SpeI          283  ACTAGT               A^CTAGT
StuI          131  AGGCCT               AGG^CCT
Tsp45I        616  GTCAC                ^GT[GC]AC
XmnI           55  GAATTAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcsI          196  AAATTC               [GA]^AATT[CT]
              472  GAATTT              
AfaI          156  GTAC                 GT^AC
              568  GTAC                
ApoI          196  AAATTC               [GA]^AATT[CT]
              472  GAATTT              
BfaI          284  CTAG                 C^TAG
              596  CTAG                
BssKI         180  CCCGG                ^CC[ACGT]GG
              432  CCAGG               
BstF5I        183  GGATG                GGATG(2/0)
              435  GGATG               
BstXI         384  CCAAAACACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              432  CCAGGATGGTGG        
BstYI         394  GGATCC               [GA]^GATC[CT]
              592  GGATCT              
DdeI           90  CTTAG                C^T[ACGT]AG
              231  CTCAG               
DpnII         395  GATC                 ^GATC
              593  GATC                
FokI          183  GGATG                GGATG(9/13)
              435  GGATG               
MaeI          284  CTAG                 C^TAG
              596  CTAG                
MaeIII        616  GTCAC                ^GT[ACGT]AC
              640  GTTAC               
MboI          395  GATC                 ^GATC
              593  GATC                
MboII         511  GAAGA                GAAGA(8/7)
              626  GAAGA               
MflI          394  GGATCC               [GA]^GATC[CT]
              592  GGATCT              
NdeII         395  GATC                 ^GATC
              593  GATC                
NlaIV         394  GGATCC               GG[ACGT]^[ACGT]CC
              442  GGTTCC              
RsaI          156  GTAC                 GT^AC
              568  GTAC                
Sau3AI        395  GATC                 ^GATC
              593  GATC                
ScrFI         180  CCCGG                CC^[ACGT]GG
              432  CCAGG               
XhoII         394  GGATCC               [GA]^GATC[CT]
              592  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   10 enzymes) #
#########################################

AlwI        BsrI        HaeIII      MnlI        MseI        
NlaIII      SfaNI       Tru9I       Tsp509I     TspRI       
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