Restriction map for pF1KB8894
 (Linear) (Six-base) MAPSORT of: KIBB8894.seq  Check: 6309  from: 1  to: 651



KIBB8894.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     101     651 
   Size:        101     550
 
ApaI G_GGCC'C

Cuts at:      0     266     636     651 
   Size:        266     370      15

  Fragments arranged by size: 

                 370     266      15
 
ApaLI G'TGCA_C

Cuts at:      0      37     651 
   Size:         37     614
 
AvaI C'yCGr_G

Cuts at:      0     151     196     267     651 
   Size:        151      45      71     384

  Fragments arranged by size: 

                 384     151      71      45
 
BanI G'GyrC_C

Cuts at:      0     211     246     256     304     651 
   Size:        211      35      10      48     347

  Fragments arranged by size: 

                 347     211      48      35      10
 
BanII G_rGCy'C

Cuts at:      0     266     636     644     651 
   Size:        266     370       8       7

  Fragments arranged by size: 

                 370     266       8       7
 
BbeI G_GCGC'C

Cuts at:      0     215     260     651 
   Size:        215      45     391

  Fragments arranged by size: 

                 391     215      45
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     523     651 
   Size:        523     128
 
BglI GCCn_nnn'nGGC

Cuts at:      0     502     611     651 
   Size:        502     109      40

  Fragments arranged by size: 

                 502     109      40
 
Bme1580I G_kGCm'C

Cuts at:      0      41     266     636     651 
   Size:         41     225     370      15

  Fragments arranged by size: 

                 370     225      41      15
 
BmgBI CAC'GTC

Cuts at:      0     525     627     651 
   Size:        525     102      24

  Fragments arranged by size: 

                 525     102      24
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     628     651 
   Size:        628      23
 
BsaHI Gr'CG_yC

Cuts at:      0     212     257     651 
   Size:        212      45     394

  Fragments arranged by size: 

                 394     212      45
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     127     547     651 
   Size:        127     420     104

  Fragments arranged by size: 

                 420     127     104
 
BsiHKAI G_wGCw'C

Cuts at:      0      41     644     651 
   Size:         41     603       7

  Fragments arranged by size: 

                 603      41       7
 
Bsp1286I G_dGCh'C

Cuts at:      0      41     266     636     644     651 
   Size:         41     225     370       8       7

  Fragments arranged by size: 

                 370     225      41       8       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     467     651 
   Size:        467     184
 
BsrBI CCG'CTC

Cuts at:      0     324     651 
   Size:        324     327
 
BsrFI r'CCGG_y

Cuts at:      0     217     651 
   Size:        217     434
 
BssSI C'ACGA_G

Cuts at:      0      43     651 
   Size:         43     608
 
BtgI C'CryG_G

Cuts at:      0     129     651 
   Size:        129     522
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     426     651 
   Size:        426     225
 
DraIII CAC_nnn'GTG

Cuts at:      0      33     651 
   Size:         33     618
 
EaeI y'GGCC_r

Cuts at:      0     144     428     464     616     651 
   Size:        144     284      36     152      35

  Fragments arranged by size: 

                 284     152     144      36      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     532     651 
   Size:        532     119
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     505     651 
   Size:        505     146
 
EcoICRI GAG'CTC

Cuts at:      0     642     651 
   Size:        642       9
 
EcoO109I rG'GnC_Cy

Cuts at:      0     263     633     651 
   Size:        263     370      18

  Fragments arranged by size: 

                 370     263      18
 
HaeII r_GCGC'y

Cuts at:      0     215     260     300     353     651 
   Size:        215      45      40      53     298

  Fragments arranged by size: 

                 298     215      53      45      40
 
KasI G'GCGC_C

Cuts at:      0     211     256     651 
   Size:        211      45     395

  Fragments arranged by size: 

                 395     211      45
 
MscI TGG'CCA

Cuts at:      0     430     466     651 
   Size:        430      36     185

  Fragments arranged by size: 

                 430     185      36
 
MspA1I CmG'CkG

Cuts at:      0      79     101     209     651 
   Size:         79      22     108     442

  Fragments arranged by size: 

                 442     108      79      22
 
NaeI GCC'GGC

Cuts at:      0     219     651 
   Size:        219     432
 
NarI GG'CG_CC

Cuts at:      0     212     257     651 
   Size:        212      45     394

  Fragments arranged by size: 

                 394     212      45
 
NcoI C'CATG_G

Cuts at:      0     129     651 
   Size:        129     522
 
NgoMIV G'CCGG_C

Cuts at:      0     217     651 
   Size:        217     434
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      33     651 
   Size:         33     618
 
PspOMI G'GGCC_C

Cuts at:      0     262     632     651 
   Size:        262     370      19

  Fragments arranged by size: 

                 370     262      19
 
PstI C_TGCA'G

Cuts at:      0     106     651 
   Size:        106     545
 
PvuII CAG'CTG

Cuts at:      0      79     651 
   Size:         79     572
 
SacI G_AGCT'C

Cuts at:      0     644     651 
   Size:        644       7
 
SfcI C'TryA_G

Cuts at:      0     102     171     189     651 
   Size:        102      69      18     462

  Fragments arranged by size: 

                 462     102      69      18
 
SfoI GGC'GCC

Cuts at:      0     213     258     651 
   Size:        213      45     393

  Fragments arranged by size: 

                 393     213      45
 
SmaI CCC'GGG

Cuts at:      0     269     651 
   Size:        269     382
 
SmlI C'TyrA_G

Cuts at:      0     643     651 
   Size:        643       8
 
StuI AGG'CCT

Cuts at:      0     496     651 
   Size:        496     155
 
StyI C'CwwG_G

Cuts at:      0     129     467     651 
   Size:        129     338     184

  Fragments arranged by size: 

                 338     184     129
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     535     651 
   Size:        535     116
 
XmaI C'CCGG_G

Cuts at:      0     267     651 
   Size:        267     384
 

 Enzymes that do cut:

    AlwNI     ApaI    ApaLI     AvaI     BanI    BanII     BbeI     BbsI
     BglI Bme1580I    BmgBI    BpuEI    BsaHI    BseRI  BsiHKAI Bsp1286I
    BspMI    BsrBI    BsrFI    BssSI     BtgI    BtgZI   DraIII     EaeI
     EarI     EciI  EcoICRI EcoO109I    HaeII     KasI     MscI   MspA1I
     NaeI     NarI     NcoI   NgoMIV    PflMI   PspOMI     PstI    PvuII
     SacI     SfcI     SfoI     SmaI     SmlI     StuI     StyI    TaqII
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI     BmrI     BmtI     BplI     BpmI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI
   BssHII   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtsI     ClaI    CspCI     DraI     DrdI     EagI  Eco57MI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MslI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmnI     ZraI
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