Restriction map for pF1KB8891
##################################
# NO CUT ENZYMES (  143 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaII       AviII       
AvrII       BalI        BamHI       BanII       BbrPI       
BbvCI       BciVI       BfrI        BglII       BlnI        
BmyI        BpmI        BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiHKAI     
BsmBI       BsmFI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrDI       
BsrGI       BsrI        BssSI       Bst1107I    BstAPI      
BstBI       BstXI       BstYI       BstZ17I     Bsu36I      
Cfr13I      ClaI        CpoI        DraII       DraIII      
DrdI        DsaI        Eam1105I    Eco47III    Eco81I      
EcoNI       EcoO109I    EcoRV       EcoT22I     FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        KspI        MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MunI        
NaeI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        ScaI        
SfiI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   68 enzymes) #
###################################

AcsI          303  GAATTC               [GA]^AATT[CT]
AcyI           90  GGCGCC               G[GA]^CG[CT]C
ApoI          303  GAATTC               [GA]^AATT[CT]
Asp700I       602  GAAAGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI           85  CCCGAG               C^[CT]CG[GA]G
BanI           90  GGCGCC               G^G[CT][GA]CC
BbeI           90  GGCGCC               GGCGC^C
BbsI          100  GAAGAC               GAAGAC(2/6)
BcgI          536  GCAGACCCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          174  TGATCA               T^GATCA
BcnI          345  CCGGG                CC^[GC]GG
BfaI          260  CTAG                 C^TAG
BglI          329  GCCAGAGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          138  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      138  GCTGAGC              GC^T[ACGT]AGC
BpuAI         100  GAAGAC               GAAGAC(2/6)
BsaHI          90  GGCGCC               G[GA]^CG[CT]C
BsiEI         114  CGGCCG               CG[GA][CT]^CG
BsiWI         464  CGTACG               C^GTACG
BsmAI         457  GAGAC                GTCTC(1/5)
BsmI          606  GCATTC               GAATGC(1/-1)
BsoBI          85  CCCGAG               C^[CT]CG[GA]G
BsrFI          70  GCCGGT               [GA]^CCGG[CT]
BssHII        125  GCGCGC               G^CGCGC
BstEII        230  GGTCACC              G^GT[ACGT]ACC
BstPI         230  GGTCACC              G^GT[ACGT]ACC
CelII         138  GCTGAGC              GC^T[ACGT]AGC
Cfr10I         70  GCCGGT               [GA]^CCGG[CT]
DpnII         175  GATC                 ^GATC
DraI          372  TTTAAA               TTT^AAA
EaeI          114  CGGCCG               [CT]^GGCC[GA]
EagI          114  CGGCCG               C^GGCCG
EarI          263  GAAGAG               CTCTTC(1/4)
EclXI         114  CGGCCG               C^GGCCG
Eco52I        114  CGGCCG               C^GGCCG
EcoO65I       230  GGTCACC              G^GT[ACGT]ACC
EcoRI         303  GAATTC               G^AATTC
EcoT14I       407  CCAAGG               C^C[AT][AT]GG
FbaI          174  TGATCA               T^GATCA
HaeII          90  GGCGCC               [GA]GCGC^[CT]
HgaI           49  GCGTC                GACGC(5/10)
Hin1I          90  GGCGCC               G[GA]^CG[CT]C
KasI           90  GGCGCC               G^GCGCC
Ksp632I       263  GAAGAG               CTCTTC(1/4)
MaeI          260  CTAG                 C^TAG
MaeII         463  ACGT                 A^CGT
MboI          175  GATC                 ^GATC
MslI          370  CATTTAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI           90  GGCGCC               GG^CGCC
NciI          345  CCGGG                CC^[GC]GG
NdeII         175  GATC                 ^GATC
NlaIV          90  GGCGCC               GG[ACGT]^[ACGT]CC
NspI          529  GCATGT               [GA]CATG^[CT]
PleI          148  GACTC                GAGTC(4/5)
PshAI         385  GACTTGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau3AI        175  GATC                 ^GATC
SexAI         581  ACCTGGT              A^CC[AT]GGT
SfaNI         448  GATGC                GCATC(5/9)
SfoI           90  GGCGCC               GGC^GCC
SspI          474  AATATT               AAT^ATT
StuI           79  AGGCCT               AGG^CCT
StyI          407  CCAAGG               C^C[AT][AT]GG
SwaI          371  ATTTAAAT             ATTT^AAAT
TaqI          182  TCGA                 T^CGA
TfiI          273  GAATC                G^A[AT]TC
Tsp509I       304  AATT                 ^AATT
TthHB8I       182  TCGA                 T^CGA
XmnI          602  GAAAGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AfaI          429  GTAC                 GT^AC
              465  GTAC                
AluI          288  AGCT                 AG^CT
              453  AGCT                
BsiYI          71  CCGGTAAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              611  CCGCTAAAAGG         
BslI           71  CCGGTAAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              611  CCGCTAAAAGG         
BsrBI         105  CCGCTC               CCGCTC(-3/-3)
              268  GAGCGG              
BstF5I         12  GGATG                GGATG(2/0)
              625  GGATG               
BstNI          30  CCTGG                CC^[AT]GG
              582  CCTGG               
EcoRII         30  CCTGG                ^CC[AT]GG
              582  CCTGG               
FokI           12  GGATG                GGATG(9/13)
              625  GGATG               
HapII          71  CCGG                 C^CGG
              345  CCGG                
HinfI         148  GACTC                G^A[ACGT]TC
              273  GAATC               
HpaII          71  CCGG                 C^CGG
              345  CCGG                
HphI          507  GGTGA                GGTGA(8/7)
              232  TCACC               
MboII         100  GAAGA                GAAGA(8/7)
              263  GAAGA               
MspI           71  CCGG                 C^CGG
              345  CCGG                
MvaI           30  CCTGG                CC^[AT]GG
              582  CCTGG               
NlaIII        192  CATG                 CATG^
              530  CATG                
PstI          284  CTGCAG               CTGCA^G
              432  CTGCAG              
PvuII         287  CAGCTG               CAG^CTG
              452  CAGCTG              
RsaI          429  GTAC                 GT^AC
              465  GTAC                
SfcI          284  CTGCAG               C^T[GA][CT]AG
              432  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   26 enzymes) #
#########################################

AccII       AciI        BbvI        BsaJI       BssKI       
BstUI       Cac8I       CfoI        DdeI        FauI        
Fnu4HI      HaeIII      HhaI        HinP1I      ItaI        
MaeIII      MnlI        MseI        MspA1I      MvnI        
MwoI        ScrFI       Tru9I       TseI        Tsp45I      
TspRI       
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