Restriction map for pF1KB8887
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

Acc65I      AccI        AclI        AcsI        AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AviII       
AvrII       BamHI       BbrPI       BbvCI       BcgI        
BciVI       BclI        BglII       BlnI        BsaAI       
BsaBI       BsaI        BsiWI       BsmI        Bsp1407I    
BspDI       BspHI       BspLU11I    BsrDI       BsrGI       
BsrI        BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DraIII      DrdI        Eam1105I    
EarI        Eco47III    Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        Ksp632I     MamI        MfeI        
MluI        MslI        MunI        NcoI        NdeI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacI        SalI        SapI        SbfI        
ScaI        SexAI       SfuI        SgrAI       SmaI        
SnaBI       SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TfiI        
Tsp509I     Tth111I     TthHB8I     Van91I      XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   65 enzymes) #
###################################

AatII         448  GACGTC               GACGT^C
AccIII        216  TCCGGA               T^CCGGA
AfaI          324  GTAC                 GT^AC
AflII          39  CTTAAG               C^TTAAG
AlwI          517  GATCC                GGATC(4/5)
AlwNI         286  CAGGTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          228  GGGCCC               GGGCC^C
AspHI          80  GAGCAC               G[AT]GC[AT]^C
BalI          371  TGGCCA               TGG^CCA
BanI          330  GGCGCC               G^G[CT][GA]CC
BbeI          330  GGCGCC               GGCGC^C
BbsI          313  GTCTTC               GAAGAC(2/6)
BfrI           39  CTTAAG               C^TTAAG
BglI          536  GCCCTGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          491  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I      491  GCTTAGC              GC^T[ACGT]AGC
BpuAI         313  GTCTTC               GAAGAC(2/6)
BsaWI         216  TCCGGA               [AT]^CCGG[AT]
BseAI         216  TCCGGA               T^CCGGA
BsgI          486  GTGCAG               GTGCAG(16/14)
BsiEI         243  CGGCCG               CG[GA][CT]^CG
BsiHKAI        80  GAGCAC               G[AT]GC[AT]^C
BsmAI         344  GAGAC                GTCTC(1/5)
BsmBI         344  GAGACG               CGTCTC(1/5)
BspEI         216  TCCGGA               T^CCGGA
BsrBI         301  GAGCGG               CCGCTC(-3/-3)
BstF5I         54  CATCC                GGATG(2/0)
BstYI         516  AGATCC               [GA]^GATC[CT]
CelII         491  GCTTAGC              GC^T[ACGT]AGC
DdeI          492  CTTAG                C^T[ACGT]AG
DpnII         517  GATC                 ^GATC
DraII         228  GGGCCCT              [GA]G^G[ACGT]CC[CT]
DsaI          168  CCGCGG               C^C[GA][CT]GG
EagI          243  CGGCCG               C^GGCCG
EclXI         243  CGGCCG               C^GGCCG
Eco52I        243  CGGCCG               C^GGCCG
EcoO109I      228  GGGCCCT              [GA]G^G[ACGT]CC[CT]
FokI           54  CATCC                GGATG(9/13)
HphI          275  TCACC                GGTGA(8/7)
KasI          330  GGCGCC               G^GCGCC
KspI          168  CCGCGG               CCGC^GG
MboI          517  GATC                 ^GATC
MboII         314  TCTTC                GAAGA(8/7)
MflI          516  AGATCC               [GA]^GATC[CT]
MluNI         371  TGGCCA               TGG^CCA
MroI          216  TCCGGA               T^CCGGA
MscI          371  TGGCCA               TGG^CCA
MseI           40  TTAA                 T^TAA
NaeI          541  GCCGGC               GCC^GGC
NarI          330  GGCGCC               GG^CGCC
NdeII         517  GATC                 ^GATC
NgoMIV        541  GCCGGC               G^CCGGC
NheI          351  GCTAGC               G^CTAGC
NlaIII         69  CATG                 CATG^
PstI          190  CTGCAG               CTGCA^G
RsaI          324  GTAC                 GT^AC
SacII         168  CCGCGG               CCGC^GG
Sau3AI        517  GATC                 ^GATC
SfcI          190  CTGCAG               C^T[GA][CT]AG
SfiI          535  GGCCCTGCCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          330  GGCGCC               GGC^GCC
SpeI          154  ACTAGT               A^CTAGT
Tru9I          40  TTAA                 T^TAA
XcmI          374  CCAACGCGCAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         516  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AcyI          330  GGCGCC               G[GA]^CG[CT]C
              448  GACGTC              
BanII          16  GAGCCC               G[GA]GC[CT]^C
              228  GGGCCC              
BsaHI         330  GGCGCC               G[GA]^CG[CT]C
              448  GACGTC              
BspMI         211  GCAGGT               ACCTGC(4/8)
              381  GCAGGT              
BsrFI         404  ACCGGC               [GA]^CCGG[CT]
              541  GCCGGC              
BstAPI         82  GCACCCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              282  GCAACAGGTGC         
Cfr10I        404  ACCGGC               [GA]^CCGG[CT]
              541  GCCGGC              
EaeI          243  CGGCCG               [CT]^GGCC[GA]
              371  TGGCCA              
FauI           63  CCCGC                CCCGC(4/6)
              207  GCGGG               
Hin1I         330  GGCGCC               G[GA]^CG[CT]C
              448  GACGTC              
MaeII         124  ACGT                 A^CGT
              449  ACGT                
MaeIII        261  GTCAC                ^GT[ACGT]AC
              388  GTCAC               
MspA1I        130  CCGCTG               C[AC]G^C[GT]G
              168  CCGCGG              
SfaNI         438  GATGC                GCATC(5/9)
               44  GCATC               
SmlI           39  CTTAAG               C^T[CT][GA]AG
              418  CTCAAG              
Tsp45I        261  GTCAC                ^GT[GC]AC
              388  GTCAC               
TspRI         267  CACTG                CA[GC]TG[ACGT][ACGT]^
              497  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AccII       AciI        AluI        AvaI        AvaII       
BbvI        BcnI        BfaI        BmyI        BpmI        
BsaJI       BseRI       BsiYI       BslI        BsmFI       
BsoBI       Bsp1286I    BssHII      BssKI       BstNI       
BstUI       Cac8I       CfoI        Cfr13I      EcoRII      
Fnu4HI      HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       ItaI        
MaeI        MnlI        MspI        MvaI        MvnI        
MwoI        NciI        NlaIV       PleI        Sau96I      
ScrFI       TseI        
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