Restriction map for pF1KB8887
 (Linear) (Six-base) MAPSORT of: KIBB8887.seq  Check: 569  from: 1  to: 594



KIBB8887.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     372     594 
   Size:        372     222
 
AatII G_ACGT'C

Cuts at:      0     452     594 
   Size:        452     142
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     221     594 
   Size:        221     373
 
AflII C'TTAA_G

Cuts at:      0      39     594 
   Size:         39     555
 
AlwNI CAG_nnn'CTG

Cuts at:      0     291     594 
   Size:        291     303
 
ApaI G_GGCC'C

Cuts at:      0     232     594 
   Size:        232     362
 
AvaI C'yCGr_G

Cuts at:      0       6      77     502     594 
   Size:          6      71     425      92

  Fragments arranged by size: 

                 425      92      71       6
 
BanI G'GyrC_C

Cuts at:      0     330     594 
   Size:        330     264
 
BanII G_rGCy'C

Cuts at:      0      20     232     594 
   Size:         20     212     362

  Fragments arranged by size: 

                 362     212      20
 
BbeI G_GCGC'C

Cuts at:      0     334     594 
   Size:        334     260
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     306     594 
   Size:        306     288
 
BglI GCCn_nnn'nGGC

Cuts at:      0     542     594 
   Size:        542      52
 
BlpI GC'TnA_GC

Cuts at:      0     492     594 
   Size:        492     102
 
Bme1580I G_kGCm'C

Cuts at:      0     232     594 
   Size:        232     362
 
BmtI G_CTAG'C

Cuts at:      0     355     594 
   Size:        355     239
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     433     594 
   Size:        433     161
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     313     319     497     594 
   Size:        163     150       6     178      97

  Fragments arranged by size: 

                 178     163     150      97       6
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     403     594 
   Size:        403     191
 
BsaHI Gr'CG_yC

Cuts at:      0     331     449     594 
   Size:        331     118     145

  Fragments arranged by size: 

                 331     145     118
 
BsaWI w'CCGG_w

Cuts at:      0     216     594 
   Size:        216     378
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     160     448     589     594 
   Size:        160     288     141       5

  Fragments arranged by size: 

                 288     160     141       5
 
BseYI C'CCAG_C

Cuts at:      0     225     594 
   Size:        225     369
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     507     594 
   Size:        507      87
 
BsiEI CG_ry'CG

Cuts at:      0     246     594 
   Size:        246     348
 
BsiHKAI G_wGCw'C

Cuts at:      0      84     594 
   Size:         84     510
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     338     594 
   Size:        338     256
 
Bsp1286I G_dGCh'C

Cuts at:      0      20      84     232     594 
   Size:         20      64     148     362

  Fragments arranged by size: 

                 362     148      64      20
 
BspEI T'CCGG_A

Cuts at:      0     216     594 
   Size:        216     378
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     202     372     594 
   Size:        202     170     222

  Fragments arranged by size: 

                 222     202     170
 
BsrBI CCG'CTC

Cuts at:      0     303     594 
   Size:        303     291
 
BsrFI r'CCGG_y

Cuts at:      0     404     541     594 
   Size:        404     137      53

  Fragments arranged by size: 

                 404     137      53
 
BssHII G'CGCG_C

Cuts at:      0     137     181     183     441     594 
   Size:        137      44       2     258     153

  Fragments arranged by size: 

                 258     153     137      44       2
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      88     288     594 
   Size:         88     200     306

  Fragments arranged by size: 

                 306     200      88
 
BstYI r'GATC_y

Cuts at:      0     516     594 
   Size:        516      78
 
BtgI C'CryG_G

Cuts at:      0     168     594 
   Size:        168     426
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      30     440     594 
   Size:         30     410     154

  Fragments arranged by size: 

                 410     154      30
 
BtsI GCAGTG_nn'

Cuts at:      0     266     496     594 
   Size:        266     230      98

  Fragments arranged by size: 

                 266     230      98
 
EaeI y'GGCC_r

Cuts at:      0     243     371     594 
   Size:        243     128     223

  Fragments arranged by size: 

                 243     223     128
 
EagI C'GGCC_G

Cuts at:      0     243     594 
   Size:        243     351
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     221     313     319     497     594 
   Size:        163      58      92       6     178      97

  Fragments arranged by size: 

                 178     163      97      92      58       6
 
EcoO109I rG'GnC_Cy

Cuts at:      0     229     594 
   Size:        229     365
 
HaeII r_GCGC'y

Cuts at:      0     309     334     512     594 
   Size:        309      25     178      82

  Fragments arranged by size: 

                 309     178      82      25
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     439     471     594 
   Size:        439      32     123

  Fragments arranged by size: 

                 439     123      32
 
KasI G'GCGC_C

Cuts at:      0     330     594 
   Size:        330     264
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     594 
   Size:        145     449
 
MscI TGG'CCA

Cuts at:      0     373     594 
   Size:        373     221
 
MspA1I CmG'CkG

Cuts at:      0     132     170     594 
   Size:        132      38     424

  Fragments arranged by size: 

                 424     132      38
 
NaeI GCC'GGC

Cuts at:      0     543     594 
   Size:        543      51
 
NarI GG'CG_CC

Cuts at:      0     331     594 
   Size:        331     263
 
NgoMIV G'CCGG_C

Cuts at:      0     541     594 
   Size:        541      53
 
NheI G'CTAG_C

Cuts at:      0     351     594 
   Size:        351     243
 
PspOMI G'GGCC_C

Cuts at:      0     228     594 
   Size:        228     366
 
PstI C_TGCA'G

Cuts at:      0     194     594 
   Size:        194     400
 
SacII CC_GC'GG

Cuts at:      0     171     594 
   Size:        171     423
 
SfcI C'TryA_G

Cuts at:      0     190     594 
   Size:        190     404
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     542     594 
   Size:        542      52
 
SfoI GGC'GCC

Cuts at:      0     332     594 
   Size:        332     262
 
SmlI C'TyrA_G

Cuts at:      0      39     418     594 
   Size:         39     379     176

  Fragments arranged by size: 

                 379     176      39
 
SpeI A'CTAG_T

Cuts at:      0     154     594 
   Size:        154     440
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     469     501     594 
   Size:        469      32      93

  Fragments arranged by size: 

                 469      93      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     381     594 
   Size:        381     213
 
ZraI GAC'GTC

Cuts at:      0     450     594 
   Size:        450     144
 

 Enzymes that do cut:

     AarI    AatII     AcuI    AflII    AlwNI     ApaI     AvaI     BanI
    BanII     BbeI     BbsI     BglI     BlpI Bme1580I     BmtI     BplI
     BpmI    BpuEI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI   BssHII
   BstAPI    BstYI     BtgI    BtgZI     BtsI     EaeI     EagI  Eco57MI
 EcoO109I    HaeII    Hin4I     KasI     MmeI     MscI   MspA1I     NaeI
     NarI   NgoMIV     NheI   PspOMI     PstI    SacII     SfcI     SfiI
     SfoI     SmlI     SpeI     TstI     XcmI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI     AleI
     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
    BmgBI     BmrI   Bpu10I     BsaI    BsaAI    BsaBI    BsaXI    BsiWI
     BsmI    BspHI    BsrDI    BsrGI    BssSI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MslI
     NcoI     NdeI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SgfI    SgrAI     SmaI
    SnaBI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI
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