Restriction map for pF1KB8880
##################################
# NO CUT ENZYMES (  198 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        AluI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BbvI        BcgI        BclI        BfrI        BglI        
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        Fnu4HI      
FseI        FspI        HaeII       HaeIII      HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HphI        ItaI        KasI        KpnI        
Ksp632I     KspI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MslI        MspA1I      
MunI        MvnI        MwoI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TseI        Tsp45I      Tth111I     TthHB8I     XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI          190  GTAGAC               GT^[AC][GT]AC
AciI          367  GCGG                 CCGC(-3/-1)
AcsI           13  GAATTT               [GA]^AATT[CT]
AlwI           79  GGATC                GGATC(4/5)
ApoI           13  GAATTT               [GA]^AATT[CT]
Asp700I       286  GAAGGTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII          85  GGTCC                G^G[AT]CC
BbsI          238  GAAGAC               GAAGAC(2/6)
BciVI         336  GGATAC               GTATCC(6/5)
BcnI          327  CCCGG                CC^[GC]GG
BfaI          472  CTAG                 C^TAG
BglII         488  AGATCT               A^GATCT
BpuAI         238  GAAGAC               GAAGAC(2/6)
BsaJI         326  CCCCGG               C^C[ACGT][ACGT]GG
BsrI          254  ACTGG                ACTGG(1/-1)
BstNI          76  CCTGG                CC^[AT]GG
BstYI         488  AGATCT               [GA]^GATC[CT]
Cac8I          33  GCTGGC               GC[ACGT]^[ACGT]GC
Cfr13I         85  GGTCC                G^G[ACGT]CC
EcoNI          76  CCTGGATCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII         76  CCTGG                ^CC[AT]GG
HapII         328  CCGG                 C^CGG
HgaI           56  GCGTC                GACGC(5/10)
HinfI         150  GAATC                G^A[ACGT]TC
HpaII         328  CCGG                 C^CGG
MaeI          472  CTAG                 C^TAG
MaeII          97  ACGT                 A^CGT
MflI          488  AGATCT               [GA]^GATC[CT]
MnlI          413  GAGG                 CCTC(7/6)
MspI          328  CCGG                 C^CGG
MvaI           76  CCTGG                CC^[AT]GG
NciI          327  CCCGG                CC^[GC]GG
NlaIII        161  CATG                 CATG^
PstI           49  CTGCAG               CTGCA^G
Sau96I         85  GGTCC                G^G[ACGT]CC
SfaNI         245  GCATC                GCATC(5/9)
TfiI          150  GAATC                G^A[AT]TC
XbaI          471  TCTAGA               T^CTAGA
XhoII         488  AGATCT               [GA]^GATC[CT]
XmnI          286  GAAGGTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

BssKI          76  CCTGG                ^CC[ACGT]GG
              327  CCCGG               
BstF5I        330  GGATG                GGATG(2/0)
              461  GGATG               
DdeI           38  CTCAG                C^T[ACGT]AG
              126  CTCAG               
DpnII          80  GATC                 ^GATC
              489  GATC                
FokI          330  GGATG                GGATG(9/13)
              461  GGATG               
MaeIII        268  GTAAC                ^GT[ACGT]AC
              478  GTTAC               
MboI           80  GATC                 ^GATC
              489  GATC                
MboII         238  GAAGA                GAAGA(8/7)
              260  GAAGA               
MseI          176  TTAA                 T^TAA
              251  TTAA                
NdeII          80  GATC                 ^GATC
              489  GATC                
PflMI          70  CCACAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              216  CCAAGTGATGG         
Sau3AI         80  GATC                 ^GATC
              489  GATC                
ScrFI          76  CCTGG                CC^[ACGT]GG
              327  CCCGG               
SfcI           49  CTGCAG               C^T[GA][CT]AG
               61  CTACAG              
Tru9I         176  TTAA                 T^TAA
              251  TTAA                
TspRI          52  CAGTG                CA[GC]TG[ACGT][ACGT]^
              195  CACTG               
Van91I         70  CCACAACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              216  CCAAGTGATGG         


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

BsiYI       BslI        Tsp509I     
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