Restriction map for pF1KB8873
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AvaII       AviII       AvrII       
BalI        BamHI       BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BsgI        BsiWI       BsmAI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HinfI       HpaI        KpnI        Ksp632I     
MaeI        MaeII       MamI        MboII       MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MunI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    RcaI        RsrII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TfiI        Tru9I       
Tsp509I     TspRI       Van91I      XbaI        XhoI        
XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AcyI          378  GGCGCC               G[GA]^CG[CT]C
AfaI          330  GTAC                 GT^AC
AlwNI         115  CAGCACCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         307  GAGCTC               G[AT]GC[AT]^C
AspI           90  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          386  CCCGGG               C^[CT]CG[GA]G
BbeI          378  GGCGCC               GGCGC^C
BsaHI         378  GGCGCC               G[GA]^CG[CT]C
BsiEI         481  CGATCG               CG[GA][CT]^CG
BsiHKAI       307  GAGCTC               G[AT]GC[AT]^C
BsmFI         421  GTCCC                GGGAC(10/14)
BsoBI         386  CCCGGG               C^[CT]CG[GA]G
BspMI         119  ACCTGC               ACCTGC(4/8)
BstEII         87  GGTGACC              G^GT[ACGT]ACC
BstPI          87  GGTGACC              G^GT[ACGT]ACC
DdeI          282  CTCAG                C^T[ACGT]AG
EaeI          434  TGGCCG               [CT]^GGCC[GA]
EcoNI         129  CCTGTCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I        87  GGTGACC              G^GT[ACGT]ACC
EcoT14I       134  CCAAGG               C^C[AT][AT]GG
Hin1I         378  GGCGCC               G[GA]^CG[CT]C
KasI          378  GGCGCC               G^GCGCC
KspI          183  CCGCGG               CCGC^GG
MslI           93  CATGTCGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          378  GGCGCC               GG^CGCC
PflFI          90  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuI          481  CGATCG               CGAT^CG
RsaI          330  GTAC                 GT^AC
SacI          307  GAGCTC               GAGCT^C
SacII         183  CCGCGG               CCGC^GG
SfaNI         475  GATGC                GCATC(5/9)
SfoI          378  GGCGCC               GGC^GCC
SgrAI         163  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI          386  CCCGGG               CCC^GGG
SmlI          175  CTCAAG               C^T[CT][GA]AG
StuI           26  AGGCCT               AGG^CCT
StyI          134  CCAAGG               C^C[AT][AT]GG
TaqI          311  TCGA                 T^CGA
Tth111I        90  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       311  TCGA                 T^CGA
XcmI          293  CCAGCATGAAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         386  CCCGGG               C^CCGGG
XmaI          386  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

ApaI          390  GGGCCC               GGGCC^C
              412  GGGCCC              
BanI           61  GGCACC               G^G[CT][GA]CC
              378  GGCGCC              
BpmI          235  CTGGAG               CTGGAG(16/14)
              304  CTGGAG              
BsrFI         164  GCCGGC               [GA]^CCGG[CT]
              403  GCCGGC              
BstF5I         75  GGATG                GGATG(2/0)
              474  GGATG               
BstNI         408  CCTGG                CC^[AT]GG
              424  CCAGG               
Cfr10I        164  GCCGGC               [GA]^CCGG[CT]
              403  GCCGGC              
DpnII         144  GATC                 ^GATC
              482  GATC                
DsaI          183  CCGCGG               C^C[GA][CT]GG
              374  CCGTGG              
EcoRII        408  CCTGG                ^CC[AT]GG
              424  CCAGG               
FokI           75  GGATG                GGATG(9/13)
              474  GGATG               
MaeIII         88  GTGAC                ^GT[ACGT]AC
              217  GTGAC               
MboI          144  GATC                 ^GATC
              482  GATC                
MvaI          408  CCTGG                CC^[AT]GG
              424  CCAGG               
NaeI          164  GCCGGC               GCC^GGC
              403  GCCGGC              
NdeII         144  GATC                 ^GATC
              482  GATC                
NgoMIV        164  GCCGGC               G^CCGGC
              403  GCCGGC              
NlaIII         93  CATG                 CATG^
              297  CATG                
PstI          256  CTGCAG               CTGCA^G
              340  CTGCAG              
PvuII         151  CAGCTG               CAG^CTG
              198  CAGCTG              
Sau3AI        144  GATC                 ^GATC
              482  GATC                
SfcI          256  CTGCAG               C^T[GA][CT]AG
              340  CTGCAG              
Tsp45I         88  GTGAC                ^GT[GC]AC
              217  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AccII       AciI        AluI        BanII       BbvI        
BcnI        BmyI        BsaJI       BseRI       BsiYI       
BslI        Bsp1286I    BssKI       BstUI       Cac8I       
CfoI        Cfr13I      DraII       EcoO109I    FauI        
Fnu4HI      HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HpaII       HphI        ItaI        
MnlI        MspA1I      MspI        MvnI        MwoI        
NciI        NlaIV       Sau96I      ScrFI       TseI        
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