Restriction map for pF1KB8873
 (Linear) (Six-base) MAPSORT of: KIBB8873.seq  Check: 9800  from: 1  to: 486



KIBB8873.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     128     486 
   Size:        128     358
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     486 
   Size:        175     311
 
AlwNI CAG_nnn'CTG

Cuts at:      0     120     486 
   Size:        120     366
 
ApaI G_GGCC'C

Cuts at:      0     394     416     486 
   Size:        394      22      70

  Fragments arranged by size: 

                 394      70      22
 
AvaI C'yCGr_G

Cuts at:      0     386     486 
   Size:        386     100
 
BanI G'GyrC_C

Cuts at:      0      61     378     486 
   Size:         61     317     108

  Fragments arranged by size: 

                 317     108      61
 
BanII G_rGCy'C

Cuts at:      0     311     394     416     486 
   Size:        311      83      22      70

  Fragments arranged by size: 

                 311      83      70      22
 
BbeI G_GCGC'C

Cuts at:      0     382     486 
   Size:        382     104
 
Bme1580I G_kGCm'C

Cuts at:      0     394     416     486 
   Size:        394      22      70

  Fragments arranged by size: 

                 394      70      22
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     325     486 
   Size:        256      69     161

  Fragments arranged by size: 

                 256     161      69
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     486 
   Size:        160     326
 
BsaHI Gr'CG_yC

Cuts at:      0     379     486 
   Size:        379     107
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      94     154     244     486 
   Size:         94      60      90     242

  Fragments arranged by size: 

                 242      94      90      60
 
BsiEI CG_ry'CG

Cuts at:      0     484     486 
   Size:        484       2
 
BsiHKAI G_wGCw'C

Cuts at:      0     311     486 
   Size:        311     175
 
Bsp1286I G_dGCh'C

Cuts at:      0     311     394     416     486 
   Size:        311      83      22      70

  Fragments arranged by size: 

                 311      83      70      22
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     128     486 
   Size:        128     358
 
BsrFI r'CCGG_y

Cuts at:      0     164     403     486 
   Size:        164     239      83

  Fragments arranged by size: 

                 239     164      83
 
BstEII G'GTnAC_C

Cuts at:      0      87     486 
   Size:         87     399
 
BtgI C'CryG_G

Cuts at:      0     183     374     486 
   Size:        183     191     112

  Fragments arranged by size: 

                 191     183     112
 
EaeI y'GGCC_r

Cuts at:      0     434     486 
   Size:        434      52
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     265     486 
   Size:        265     221
 
EcoICRI GAG'CTC

Cuts at:      0     309     486 
   Size:        309     177
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     256     325     486 
   Size:        175      81      69     161

  Fragments arranged by size: 

                 175     161      81      69
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     133     486 
   Size:        133     353
 
EcoO109I rG'GnC_Cy

Cuts at:      0     126     390     391     412     413     486 
   Size:        126     264       1      21       1      73

  Fragments arranged by size: 

                 264     126      73      21       1       1
 
HaeII r_GCGC'y

Cuts at:      0     165     340     382     486 
   Size:        165     175      42     104

  Fragments arranged by size: 

                 175     165     104      42
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     466     486 
   Size:        466      20
 
KasI G'GCGC_C

Cuts at:      0     378     486 
   Size:        378     108
 
MslI CAynn'nnrTG

Cuts at:      0      97     486 
   Size:         97     389
 
MspA1I CmG'CkG

Cuts at:      0     153     185     200     486 
   Size:        153      32      15     286

  Fragments arranged by size: 

                 286     153      32      15
 
NaeI GCC'GGC

Cuts at:      0     166     405     486 
   Size:        166     239      81

  Fragments arranged by size: 

                 239     166      81
 
NarI GG'CG_CC

Cuts at:      0     379     486 
   Size:        379     107
 
NgoMIV G'CCGG_C

Cuts at:      0     164     403     486 
   Size:        164     239      83

  Fragments arranged by size: 

                 239     164      83
 
PspOMI G'GGCC_C

Cuts at:      0     390     412     486 
   Size:        390      22      74

  Fragments arranged by size: 

                 390      74      22
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     172     204     486 
   Size:        172      32     282

  Fragments arranged by size: 

                 282     172      32
 
PstI C_TGCA'G

Cuts at:      0     260     344     486 
   Size:        260      84     142

  Fragments arranged by size: 

                 260     142      84
 
PvuI CG_AT'CG

Cuts at:      0     484     486 
   Size:        484       2
 
PvuII CAG'CTG

Cuts at:      0     153     200     486 
   Size:        153      47     286

  Fragments arranged by size: 

                 286     153      47
 
SacI G_AGCT'C

Cuts at:      0     311     486 
   Size:        311     175
 
SacII CC_GC'GG

Cuts at:      0     186     486 
   Size:        186     300
 
SfcI C'TryA_G

Cuts at:      0     256     340     486 
   Size:        256      84     146

  Fragments arranged by size: 

                 256     146      84
 
SfoI GGC'GCC

Cuts at:      0     380     486 
   Size:        380     106
 
SgrAI Cr'CCGG_yG

Cuts at:      0     164     486 
   Size:        164     322
 
SmaI CCC'GGG

Cuts at:      0     388     486 
   Size:        388      98
 
SmlI C'TyrA_G

Cuts at:      0     175     486 
   Size:        175     311
 
StuI AGG'CCT

Cuts at:      0      28     486 
   Size:         28     458
 
StyI C'CwwG_G

Cuts at:      0     134     486 
   Size:        134     352
 
Tth111I GACn'n_nGTC

Cuts at:      0      93     486 
   Size:         93     393
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     300     486 
   Size:        300     186
 
XmaI C'CCGG_G

Cuts at:      0     386     486 
   Size:        386     100
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI     ApaI     AvaI     BanI    BanII     BbeI
 Bme1580I     BpmI    BpuEI    BsaHI    BseRI    BsiEI  BsiHKAI Bsp1286I
    BspMI    BsrFI   BstEII     BtgI     EaeI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    HaeII    Hin4I     KasI     MslI   MspA1I     NaeI
     NarI   NgoMIV   PspOMI     PsrI     PstI     PvuI    PvuII     SacI
    SacII     SfcI     SfoI    SgrAI     SmaI     SmlI     StuI     StyI
  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseYI
     BsgI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI    RsrII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII     TatI     TstI     XbaI     XhoI     XmnI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com