Restriction map for pF1KB8868
##################################
# NO CUT ENZYMES (  166 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      Alw44I      
AlwI        AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AviII       AvrII       BalI        
BamHI       BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaI        BseAI       
BsgI        BsiWI       BsmAI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BsrGI       BsrI        BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DdeI        DpnII       DraI        DraIII      
DrdI        DsaI        EagI        EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       FbaI        FseI        FspI        
HgaI        HincII      HindII      HindIII     HpaI        
HphI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MaeIII      MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MslI        MunI        NcoI        NdeI        
NdeII       NheI        NotI        NspI        NspV        
PacI        PaeR7I      PflFI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        SexAI       SfcI        SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        Tru9I       Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     XbaI        XhoI        XhoII       
XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AgeI          200  ACCGGT               A^CCGGT
AhdI          232  GACACCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI          13  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspEI         232  GACACCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         271  GAGCAC               G[AT]GC[AT]^C
BanII         254  GGGCTC               G[GA]GC[CT]^C
BsaWI         200  ACCGGT               [AT]^CCGG[AT]
BseRI         112  GAGGAG               GAGGAG(10/8)
BsiHKAI       271  GAGCAC               G[AT]GC[AT]^C
BspMI         221  ACCTGC               ACCTGC(4/8)
BsrBI         363  GAGCGG               CCGCTC(-3/-3)
BssHII        283  GCGCGC               G^CGCGC
BstF5I        373  GGATG                GGATG(2/0)
BstNI         369  CCTGG                CC^[AT]GG
EaeI          119  TGGCCG               [CT]^GGCC[GA]
Eam1105I      232  GACACCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII        369  CCTGG                ^CC[AT]GG
EcoT14I       320  CCAAGG               C^C[AT][AT]GG
EcoT22I         1  ATGCAT               ATGCA^T
FokI          373  GGATG                GGATG(9/13)
HinfI         350  GAATC                G^A[ACGT]TC
MboII         150  GAAGA                GAAGA(8/7)
MspA1I         13  CAGCTG               C[AC]G^C[GT]G
MvaI          369  CCTGG                CC^[AT]GG
NaeI          105  GCCGGC               GCC^GGC
NgoMIV        105  GCCGGC               G^CCGGC
NlaIII        360  CATG                 CATG^
NruI          205  TCGCGA               TCG^CGA
NsiI            1  ATGCAT               ATGCA^T
PflMI          12  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         200  ACCGGT               A^CCGGT
PvuII          13  CAGCTG               CAG^CTG
SfaNI           3  GCATC                GCATC(5/9)
StyI          320  CCAAGG               C^C[AT][AT]GG
TfiI          350  GAATC                G^A[AT]TC
Van91I         12  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          359  CCATGAGCGGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcyI           78  GGCGCC               G[GA]^CG[CT]C
              181  GGCGCC              
AvaI           73  CCCGGG               C^[CT]CG[GA]G
              169  CCCGGG              
BanI           78  GGCGCC               G^G[CT][GA]CC
              181  GGCGCC              
BbeI           78  GGCGCC               GGCGC^C
              181  GGCGCC              
BmyI          254  GGGCTC               G[AGT]GC[ACT]^C
              271  GAGCAC              
BsaHI          78  GGCGCC               G[GA]^CG[CT]C
              181  GGCGCC              
BsiEI         198  CGACCG               CG[GA][CT]^CG
              202  CGGTCG              
BsmFI         218  GGGAC                GGGAC(10/14)
               91  GTCCC               
BsoBI          73  CCCGGG               C^[CT]CG[GA]G
              169  CCCGGG              
Bsp1286I      254  GGGCTC               G[AGT]GC[ACT]^C
              271  GAGCAC              
BsrFI         105  GCCGGC               [GA]^CCGG[CT]
              200  ACCGGT              
Cfr10I        105  GCCGGC               [GA]^CCGG[CT]
              200  ACCGGT              
DraII          89  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              218  GGGACCT             
EcoO109I       89  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              218  GGGACCT             
HaeII          78  GGCGCC               [GA]GCGC^[CT]
              181  GGCGCC              
Hin1I          78  GGCGCC               G[GA]^CG[CT]C
              181  GGCGCC              
KasI           78  GGCGCC               G^GCGCC
              181  GGCGCC              
NarI           78  GGCGCC               GG^CGCC
              181  GGCGCC              
PpuMI          89  AGGTCCC              [GA]G^G[AT]CC[CT]
              218  GGGACCT             
SfoI           78  GGCGCC               GGC^GCC
              181  GGCGCC              
SmaI           73  CCCGGG               CCC^GGG
              169  CCCGGG              
SmlI          190  CTCAAG               C^T[CT][GA]AG
              337  CTCAAG              
XmaCI          73  CCCGGG               C^CCGGG
              169  CCCGGG              
XmaI           73  CCCGGG               C^CCGGG
              169  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   31 enzymes) #
#########################################

AccII       AciI        AluI        AvaII       BbvI        
BcnI        BsaJI       BsiYI       BslI        BssKI       
BstUI       Cac8I       CfoI        Cfr13I      FauI        
Fnu4HI      HaeIII      HapII       HhaI        HinP1I      
HpaII       ItaI        MnlI        MspI        MvnI        
MwoI        NciI        NlaIV       Sau96I      ScrFI       
TseI        
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