Restriction map for pF1KB8868
 (Linear) (Six-base) MAPSORT of: KIBB8868.seq  Check: 3036  from: 1  to: 378



KIBB8868.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AgeI A'CCGG_T

Cuts at:      0     200     378 
   Size:        200     178
 
AhdI GACnn_n'nnGTC

Cuts at:      0     237     378 
   Size:        237     141
 
AlwNI CAG_nnn'CTG

Cuts at:      0      18     378 
   Size:         18     360
 
AvaI C'yCGr_G

Cuts at:      0      73     169     378 
   Size:         73      96     209

  Fragments arranged by size: 

                 209      96      73
 
BanI G'GyrC_C

Cuts at:      0      78     181     378 
   Size:         78     103     197

  Fragments arranged by size: 

                 197     103      78
 
BanII G_rGCy'C

Cuts at:      0     258     378 
   Size:        258     120
 
BbeI G_GCGC'C

Cuts at:      0      82     185     378 
   Size:         82     103     193

  Fragments arranged by size: 

                 193     103      82
 
BfrBI ATG'CAT

Cuts at:      0       3     378 
   Size:          3     375
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     322     378 
   Size:        175     147      56

  Fragments arranged by size: 

                 175     147      56
 
BsaHI Gr'CG_yC

Cuts at:      0      79     182     378 
   Size:         79     103     196

  Fragments arranged by size: 

                 196     103      79
 
BsaWI w'CCGG_w

Cuts at:      0     200     378 
   Size:        200     178
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     127     378 
   Size:        127     251
 
BseYI C'CCAG_C

Cuts at:      0      11     378 
   Size:         11     367
 
BsiEI CG_ry'CG

Cuts at:      0     201     205     378 
   Size:        201       4     173

  Fragments arranged by size: 

                 201     173       4
 
BsiHKAI G_wGCw'C

Cuts at:      0     275     378 
   Size:        275     103
 
Bsp1286I G_dGCh'C

Cuts at:      0     258     275     378 
   Size:        258      17     103

  Fragments arranged by size: 

                 258     103      17
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     230     378 
   Size:        230     148
 
BsrBI CCG'CTC

Cuts at:      0     365     378 
   Size:        365      13
 
BsrFI r'CCGG_y

Cuts at:      0     105     200     378 
   Size:        105      95     178

  Fragments arranged by size: 

                 178     105      95
 
BssHII G'CGCG_C

Cuts at:      0     283     378 
   Size:        283      95
 
EaeI y'GGCC_r

Cuts at:      0     119     378 
   Size:        119     259
 
EcoO109I rG'GnC_Cy

Cuts at:      0      90     219     378 
   Size:         90     129     159

  Fragments arranged by size: 

                 159     129      90
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     330     345     378 
   Size:        330      15      33

  Fragments arranged by size: 

                 330      33      15
 
HaeII r_GCGC'y

Cuts at:      0      82     185     378 
   Size:         82     103     193

  Fragments arranged by size: 

                 193     103      82
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     223     255     378 
   Size:        223      32     123

  Fragments arranged by size: 

                 223     123      32
 
KasI G'GCGC_C

Cuts at:      0      78     181     378 
   Size:         78     103     197

  Fragments arranged by size: 

                 197     103      78
 
MspA1I CmG'CkG

Cuts at:      0      15     378 
   Size:         15     363
 
NaeI GCC'GGC

Cuts at:      0     107     378 
   Size:        107     271
 
NarI GG'CG_CC

Cuts at:      0      79     182     378 
   Size:         79     103     196

  Fragments arranged by size: 

                 196     103      79
 
NgoMIV G'CCGG_C

Cuts at:      0     105     378 
   Size:        105     273
 
NruI TCG'CGA

Cuts at:      0     207     378 
   Size:        207     171
 
NsiI A_TGCA'T

Cuts at:      0       5     378 
   Size:          5     373
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      18     378 
   Size:         18     360
 
PfoI T'CCnGG_A

Cuts at:      0     215     378 
   Size:        215     163
 
PpuMI rG'GwC_Cy

Cuts at:      0      90     219     378 
   Size:         90     129     159

  Fragments arranged by size: 

                 159     129      90
 
PvuII CAG'CTG

Cuts at:      0      15     378 
   Size:         15     363
 
SfoI GGC'GCC

Cuts at:      0      80     183     378 
   Size:         80     103     195

  Fragments arranged by size: 

                 195     103      80
 
SmaI CCC'GGG

Cuts at:      0      75     171     378 
   Size:         75      96     207

  Fragments arranged by size: 

                 207      96      75
 
SmlI C'TyrA_G

Cuts at:      0     190     337     378 
   Size:        190     147      41

  Fragments arranged by size: 

                 190     147      41
 
StyI C'CwwG_G

Cuts at:      0     320     378 
   Size:        320      58
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     362     378 
   Size:        362      16
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     366     378 
   Size:        366      12
 
XmaI C'CCGG_G

Cuts at:      0      73     169     378 
   Size:         73      96     209

  Fragments arranged by size: 

                 209      96      73
 

 Enzymes that do cut:

     AgeI     AhdI    AlwNI     AvaI     BanI    BanII     BbeI    BfrBI
    BpuEI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I
    BspMI    BsrBI    BsrFI   BssHII     EaeI EcoO109I     FalI    HaeII
    Hin4I     KasI   MspA1I     NaeI     NarI   NgoMIV     NruI     NsiI
    PflMI     PfoI    PpuMI    PvuII     SfoI     SmaI     SmlI     StyI
    TaqII     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaXI
     BsgI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NcoI     NdeI     NheI     NotI
     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmnI
     ZraI
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