Restriction map for pF1KB8865
 (Circular) (Six-base) MAPSORT of: pF1KB8865.seq  Check: 7857  from: 1  to: 3452



pF1KB8865  3452 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:30  ..

AccI GT'mk_AC

Cuts at:    490     490 
   Size:       3452
 
Acc65I G'GTAC_C

Cuts at:    468     468 
   Size:       3452
 
AclI AA'CG_TT

Cuts at:   3292    3292 
   Size:       3452
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1277    1709    2456    2724    1277 
   Size:        432     747     268    2005

  Fragments arranged by size: 

                2005     747     432     268
 
AflIII A'CryG_T

Cuts at:    804    1908    2885     804 
   Size:       1104     977    1371

  Fragments arranged by size: 

                1371    1104     977
 
AgeI A'CCGG_T

Cuts at:   1840    1840 
   Size:       3452
 
AlwNI CAG_nnn'CTG

Cuts at:    757    2324     757 
   Size:       1567    1885
 
ApaLI G'TGCA_C

Cuts at:   2222    2222 
   Size:       3452
 
ApoI r'AATT_y

Cuts at:    240     456    2805    3061     240 
   Size:        216    2349     256     631

  Fragments arranged by size: 

                2349     631     256     216
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3452
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3452
 
AvaI C'yCGr_G

Cuts at:    472     472 
   Size:       3452
 
BamHI G'GATC_C

Cuts at:    477     477 
   Size:       3452
 
BanI G'GyrC_C

Cuts at:    468     882    1130    1165     468 
   Size:        414     248      35    2755

  Fragments arranged by size: 

                2755     414     248      35
 
BanII G_rGCy'C

Cuts at:    466    1496     466 
   Size:       1030    2422
 
BbeI G_GCGC'C

Cuts at:    886    1134     886 
   Size:        248    3204
 
BciVI GTATCCnnnnn_n'

Cuts at:   1350    2111    3452    1350 
   Size:        761    1341    1350

  Fragments arranged by size: 

                1350    1341     761
 
BclI T'GATC_A

Cuts at:    972     972 
   Size:       3452
 
BglII A'GATC_T

Cuts at:    967    3004     967 
   Size:       2037    1415
 
BlpI GC'TnA_GC

Cuts at:    564     564 
   Size:       3452
 
Bme1580I G_kGCm'C

Cuts at:   1077    1170    2226    1077 
   Size:         93    1056    2303

  Fragments arranged by size: 

                2303    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    401     664     745    1080    3224     401 
   Size:        263      81     335    2144     629

  Fragments arranged by size: 

                2144     629     335     263      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1819    1819 
   Size:       3452
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    628    1507    1999    2297     628 
   Size:        879     492     298    1783

  Fragments arranged by size: 

                1783     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3156    3156 
   Size:       3452
 
BsaAI yAC'GTr

Cuts at:    805     805 
   Size:       3452
 
BsaBI GATnn'nnATC

Cuts at:      6     966    3003       6 
   Size:        960    2037     455

  Fragments arranged by size: 

                2037     960     455
 
BsaHI Gr'CG_yC

Cuts at:    883    1131    1833    3329     883 
   Size:        248     702    1496    1006

  Fragments arranged by size: 

                1496    1006     702     248
 
BsaWI w'CCGG_w

Cuts at:    641    1162    1840    2114    2261    2672     641 
   Size:        521     678     274     147     411    1421

  Fragments arranged by size: 

                1421     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    877    1635    2212    2708     877 
   Size:        758     577     496    1621

  Fragments arranged by size: 

                1621     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     994    1040    2248     104 
   Size:        890      46    1208    1308

  Fragments arranged by size: 

                1308    1208     890      46
 
BsiHKAI G_wGCw'C

Cuts at:    466    1244    1434    2226     466 
   Size:        778     190     792    1692

  Fragments arranged by size: 

                1692     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    395     395 
   Size:       3452
 
Bsp1286I G_dGCh'C

Cuts at:    466    1077    1170    1244    1434    1496    2226     466 
   Size:        611      93      74     190      62     730    1692

  Fragments arranged by size: 

                1692     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2672    2672 
   Size:       3452
 
BspHI T'CATG_A

Cuts at:   2628    2628 
   Size:       3452
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    502     716    1018    1399     502 
   Size:        214     302     381    2555

  Fragments arranged by size: 

                2555     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1744    1798    3282    3447      50 
   Size:       1694      54    1484     165      55

  Fragments arranged by size: 

                1694    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1364    1364 
   Size:       3452
 
BsrFI r'CCGG_y

Cuts at:   1450    1631    1840    1450 
   Size:        181     209    3062

  Fragments arranged by size: 

                3062     209     181
 
BssSI C'ACGA_G

Cuts at:   1723    2081    1723 
   Size:        358    3094
 
BstAPI GCAn_nnn'nTGC

Cuts at:    829     829 
   Size:       3452
 
BstBI TT'CG_AA

Cuts at:   1813    1813 
   Size:       3452
 
BstXI CCAn_nnnn'nTGG

Cuts at:    121     121 
   Size:       3452
 
BstYI r'GATC_y

Cuts at:      1     477     944     967    1302    1548    2560    2646    2658 
   Size:        476     467      23     335     246    1012      86      12

Cuts at:   2658    3004       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     476     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    287     287 
   Size:       3452
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1402    1583    1746    1402 
   Size:        181     163    3108

  Fragments arranged by size: 

                3108     181     163
 
BtsI GCAGTG_nn'

Cuts at:    695     695 
   Size:       3452
 
DraI TTT'AAA

Cuts at:    128     451     128 
   Size:        323    3129
 
DrdI GACnn_nn'nnGTC

Cuts at:   1158    2016    1158 
   Size:        858    2594
 
EaeI y'GGCC_r

Cuts at:   1037    1211    1602    1629    2974    1037 
   Size:        174     391      27    1345    1515

  Fragments arranged by size: 

                1515    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1037    1037 
   Size:       3452
 
EarI CTCTTCn'nnn_

Cuts at:    188    1475    1685     188 
   Size:       1287     210    1955

  Fragments arranged by size: 

                1955    1287     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1660    1970    2116    3262    1660 
   Size:        310     146    1146    1850

  Fragments arranged by size: 

                1850    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    464     464 
   Size:       3452
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1277    1709    2456    2724    1277 
   Size:        432     747     268    2005

  Fragments arranged by size: 

                2005     747     432     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:    356     356 
   Size:       3452
 
EcoO109I rG'GnC_Cy

Cuts at:    591     591 
   Size:       3452
 
EcoRI G'AATT_C

Cuts at:    456     456 
   Size:       3452
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1471    1471 
   Size:       3452
 
FspI TGC'GCA

Cuts at:   1233    1233 
   Size:       3452
 
HaeII r_GCGC'y

Cuts at:    886    1134    2156    3135     886 
   Size:        248    1022     979    1203

  Fragments arranged by size: 

                1203    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1294    1324    1326    1356    1294 
   Size:         30       2      30    3390

  Fragments arranged by size: 

                3390      30      30       2
 
HincII GTy'rAC

Cuts at:    491     491 
   Size:       3452
 
KasI G'GCGC_C

Cuts at:    882    1130     882 
   Size:        248    3204
 
KpnI G_GTAC'C

Cuts at:    472     472 
   Size:       3452
 
MluI A'CGCG_T

Cuts at:   2885    2885 
   Size:       3452
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    426    2123    2307     426 
   Size:       1697     184    1571

  Fragments arranged by size: 

                1697    1571     184
 
MscI TGG'CCA

Cuts at:   1213    1213 
   Size:       3452
 
MslI CAynn'nnrTG

Cuts at:   1441    1568    1441 
   Size:        127    3325
 
MspA1I CmG'CkG

Cuts at:    563     877    1237    2250    2495     563 
   Size:        314     360    1013     245    1520

  Fragments arranged by size: 

                1520    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1633    1633 
   Size:       3452
 
NarI GG'CG_CC

Cuts at:    883    1131     883 
   Size:        248    3204
 
NgoMIV G'CCGG_C

Cuts at:   1631    1631 
   Size:       3452
 
NspI r_CATG'y

Cuts at:    505    1005    1912     505 
   Size:        500     907    2045

  Fragments arranged by size: 

                2045     907     500
 
PciI A'CATG_T

Cuts at:   1908    1908 
   Size:       3452
 
PmeI GTTT'AAAC

Cuts at:    451     451 
   Size:       3452
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2591    2623    2591 
   Size:         32    3420
 
PsiI TTA'TAA

Cuts at:    428     428 
   Size:       3452
 
PstI C_TGCA'G

Cuts at:    499    1184     499 
   Size:        685    2767
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3452
 
PvuII CAG'CTG

Cuts at:    877    1237     877 
   Size:        360    3092
 
RsrII CG'GwC_CG

Cuts at:   1647    1647 
   Size:       3452
 
SacI G_AGCT'C

Cuts at:    466     466 
   Size:       3452
 
SalI G'TCGA_C

Cuts at:    489     489 
   Size:       3452
 
SapI GCTCTTCn'nnn_

Cuts at:   1475    1685    1475 
   Size:        210    3242
 
SbfI CC_TGCA'GG

Cuts at:    499     499 
   Size:       3452
 
ScaI AGT'ACT

Cuts at:   2969    2969 
   Size:       3452
 
SfcI C'TryA_G

Cuts at:     33     353     495    1180    2173    2364      33 
   Size:        320     142     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     320     191     142
 
SfoI GGC'GCC

Cuts at:    884    1132     884 
   Size:        248    3204
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3452
 
SmaI CCC'GGG

Cuts at:    474     474 
   Size:       3452
 
SmlI C'TyrA_G

Cuts at:    607    1522    2014    2276     607 
   Size:        915     492     262    1783

  Fragments arranged by size: 

                1783     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3452
 
SphI G_CATG'C

Cuts at:    505    1005     505 
   Size:        500    2952
 
StyI C'CwwG_G

Cuts at:    188     586     940     188 
   Size:        398     354    2700

  Fragments arranged by size: 

                2700     398     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1035    1168    1627    1836    1035 
   Size:        133     459     209    2651

  Fragments arranged by size: 

                2651     459     209     133
 
TatI w'GTAC_w

Cuts at:    228    2967     228 
   Size:       2739     713
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2969    3001    2969 
   Size:         32    3420
 
Tth111I GACn'n_nGTC

Cuts at:   1249    1249 
   Size:       3452
 
XbaI T'CTAG_A

Cuts at:    483     483 
   Size:       3452
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    947     947 
   Size:       3452
 
XmaI C'CCGG_G

Cuts at:    472     472 
   Size:       3452
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI   NgoMIV     NspI     PciI     PmeI     PpiI     PsiI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI
     BglI    BmgBI     BmtI     BpmI   Bpu10I    BsaXI    BseRI     BsgI
    BsiWI    BsmBI    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I     ClaI
    CspCI   DraIII    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmlI    PpuMI    PshAI   PspOMI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com