Restriction map for
pF1KB8842
(Circular) (Six-base) MAPSORT of: pF1KB8842.seq Check: 6024 from: 1 to: 4568
pF1KB8842 4568 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1289 1289
Size: 4568
AccI GT'mk_AC
Cuts at: 443 748 1606 443
Size: 305 858 3405
Fragments arranged by size:
3405 858 305
Acc65I G'GTAC_C
Cuts at: 916 1584 916
Size: 668 3900
AclI AA'CG_TT
Cuts at: 4408 4408
Size: 4568
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 2393 2825 3572 3840 494
Size: 1899 432 747 268 1222
Fragments arranged by size:
1899 1222 747 432 268
AflIII A'CryG_T
Cuts at: 1920 3024 4001 1920
Size: 1104 977 2487
Fragments arranged by size:
2487 1104 977
AgeI A'CCGG_T
Cuts at: 2956 2956
Size: 4568
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 992 992
Size: 4568
AlwNI CAG_nnn'CTG
Cuts at: 1005 1209 1873 3440 1005
Size: 204 664 1567 2133
Fragments arranged by size:
2133 1567 664 204
ApaLI G'TGCA_C
Cuts at: 3338 3338
Size: 4568
ApoI r'AATT_y
Cuts at: 1572 3921 4177 1572
Size: 2349 256 1963
Fragments arranged by size:
2349 1963 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4568
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4568
AvaI C'yCGr_G
Cuts at: 407 434 787 880 1588 407
Size: 27 353 93 708 3387
Fragments arranged by size:
3387 708 353 93 27
BamHI G'GATC_C
Cuts at: 549 1593 549
Size: 1044 3524
BanI G'GyrC_C
Cuts at: 916 1584 1998 2246 2281 916
Size: 668 414 248 35 3203
Fragments arranged by size:
3203 668 414 248 35
BanII G_rGCy'C
Cuts at: 221 1582 2612 221
Size: 1361 1030 2177
Fragments arranged by size:
2177 1361 1030
BbeI G_GCGC'C
Cuts at: 2002 2250 2002
Size: 248 4320
BbvCI CC'TCA_GC
Cuts at: 599 901 599
Size: 302 4266
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4534
BciVI GTATCCnnnnn_n'
Cuts at: 2466 3227 4568 2466
Size: 761 1341 2466
Fragments arranged by size:
2466 1341 761
BclI T'GATC_A
Cuts at: 2088 2088
Size: 4568
BglII A'GATC_T
Cuts at: 338 2083 4120 338
Size: 1745 2037 786
Fragments arranged by size:
2037 1745 786
BlpI GC'TnA_GC
Cuts at: 717 1680 717
Size: 963 3605
Bme1580I G_kGCm'C
Cuts at: 1014 1317 2193 2286 3342 1014
Size: 303 876 93 1056 2240
Fragments arranged by size:
2240 1056 876 303 93
BmrI ACTGGGnnnn_n'
Cuts at: 270 1780 1861 2196 4340 270
Size: 1510 81 335 2144 498
Fragments arranged by size:
2144 1510 498 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 813 2935 813
Size: 2122 2446
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 622 758 861 1081 1315 439
Size: 183 136 103 220 234 3692
Fragments arranged by size:
3692 234 220 183 136 103
Bpu10I CC'TnA_GC
Cuts at: 599 757 901 599
Size: 158 144 4266
Fragments arranged by size:
4266 158 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 219 1744 2623 3115 3413 133
Size: 86 1525 879 492 298 1288
Fragments arranged by size:
1525 1288 879 492 298 86
BsaI GGTCTCn'nnnn_
Cuts at: 1405 4272 1405
Size: 2867 1701
BsaAI yAC'GTr
Cuts at: 1921 1921
Size: 4568
BsaBI GATnn'nnATC
Cuts at: 6 2082 4119 6
Size: 2076 2037 455
Fragments arranged by size:
2076 2037 455
BsaHI Gr'CG_yC
Cuts at: 1999 2247 2949 4445 1999
Size: 248 702 1496 2122
Fragments arranged by size:
2122 1496 702 248
BsaWI w'CCGG_w
Cuts at: 353 1757 2278 2956 3230 3377 3788 353
Size: 1404 521 678 274 147 411 1133
Fragments arranged by size:
1404 1133 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1465 1501 1465
Size: 36 4532
BseYI C'CCAG_C
Cuts at: 1235 1993 2751 3328 3824 1235
Size: 758 758 577 496 1979
Fragments arranged by size:
1979 758 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 433 1092 1249 1461 238
Size: 195 659 157 212 3345
Fragments arranged by size:
3345 659 212 195 157
BsiEI CG_ry'CG
Cuts at: 104 819 2110 2156 3364 104
Size: 715 1291 46 1208 1308
Fragments arranged by size:
1308 1291 1208 715 46
BsiHKAI G_wGCw'C
Cuts at: 709 718 1331 1582 2360 2550 3342 709
Size: 9 613 251 778 190 792 1935
Fragments arranged by size:
1935 792 778 613 251 190 9
Bsp1286I G_dGCh'C
Cuts at: 221 709 718 1014 1317 1331 1582 2193 2286
Size: 488 9 296 303 14 251 611 93
Cuts at: 2286 2360 2550 2612 3342 221
Size: 74 190 62 730 1447
Fragments arranged by size:
1447 730 611 488 303 296 251 190
93 74 62 14 9
BspEI T'CCGG_A
Cuts at: 3788 3788
Size: 4568
BspHI T'CATG_A
Cuts at: 3744 3744
Size: 4568
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1289 1542 1618 1832 2134 2515 1289
Size: 253 76 214 302 381 3342
Fragments arranged by size:
3342 381 302 253 214 76
BsrBI CCG'CTC
Cuts at: 50 1497 2860 2914 4398 4563 50
Size: 1447 1363 54 1484 165 55
Fragments arranged by size:
1484 1447 1363 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2480 2480
Size: 4568
BsrFI r'CCGG_y
Cuts at: 1347 2566 2747 2956 1347
Size: 1219 181 209 2959
Fragments arranged by size:
2959 1219 209 181
BssSI C'ACGA_G
Cuts at: 2839 3197 2839
Size: 358 4210
BstAPI GCAn_nnn'nTGC
Cuts at: 713 1369 1945 713
Size: 656 576 3336
Fragments arranged by size:
3336 656 576
BstBI TT'CG_AA
Cuts at: 2929 2929
Size: 4568
BstEII G'GTnAC_C
Cuts at: 885 885
Size: 4568
BstXI CCAn_nnnn'nTGG
Cuts at: 546 913 546
Size: 367 4201
BstYI r'GATC_y
Cuts at: 1 338 549 1205 1593 2060 2083 2418 2664
Size: 337 211 656 388 467 23 335 246
Cuts at: 2664 3676 3762 3774 4120 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 656 467 449 388 346 337 335
246 211 86 23 12
BstZ17I GTA'TAC
Cuts at: 749 749
Size: 4568
BtgI C'CryG_G
Cuts at: 130 194 674 130
Size: 64 480 4024
Fragments arranged by size:
4024 480 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2518 2699 2862 2518
Size: 181 163 4224
Fragments arranged by size:
4224 181 163
BtsI GCAGTG_nn'
Cuts at: 565 1439 1473 1811 565
Size: 874 34 338 3322
Fragments arranged by size:
3322 874 338 34
DraI TTT'AAA
Cuts at: 1567 1567
Size: 4568
DraIII CAC_nnn'GTG
Cuts at: 713 713
Size: 4568
DrdI GACnn_nn'nnGTC
Cuts at: 2274 3132 2274
Size: 858 3710
EaeI y'GGCC_r
Cuts at: 1523 2153 2327 2718 2745 4090 1523
Size: 630 174 391 27 1345 2001
Fragments arranged by size:
2001 1345 630 391 174 27
EagI C'GGCC_G
Cuts at: 2153 2153
Size: 4568
EarI CTCTTCn'nnn_
Cuts at: 2591 2801 2591
Size: 210 4358
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2776 3086 3232 4378 2776
Size: 310 146 1146 2966
Fragments arranged by size:
2966 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1580 1580
Size: 4568
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 494 622 758 861 1081 1315 2393 2825
Size: 55 128 136 103 220 234 1078 432
Cuts at: 2825 3572 3840 439
Size: 747 268 1167
Fragments arranged by size:
1167 1078 747 432 268 234 220 136
128 103 55
EcoNI CCTnn'n_nnAGG
Cuts at: 226 1447 226
Size: 1221 3347
EcoO109I rG'GnC_Cy
Cuts at: 603 1002 1272 1707 603
Size: 399 270 435 3464
Fragments arranged by size:
3464 435 399 270
EcoRI G'AATT_C
Cuts at: 1572 1572
Size: 4568
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1008 2587 1008
Size: 1579 2989
FspI TGC'GCA
Cuts at: 2349 2349
Size: 4568
HaeII r_GCGC'y
Cuts at: 2002 2250 3272 4251 2002
Size: 248 1022 979 2319
Fragments arranged by size:
2319 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2410 2440 2442 2472 2410
Size: 30 2 30 4506
Fragments arranged by size:
4506 30 30 2
HincII GTy'rAC
Cuts at: 264 1607 264
Size: 1343 3225
HpaI GTT'AAC
Cuts at: 264 264
Size: 4568
KasI G'GCGC_C
Cuts at: 1998 2246 1998
Size: 248 4320
KpnI G_GTAC'C
Cuts at: 920 1588 920
Size: 668 3900
MluI A'CGCG_T
Cuts at: 4001 4001
Size: 4568
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1581 3239 3423 1581
Size: 1658 184 2726
Fragments arranged by size:
2726 1658 184
MscI TGG'CCA
Cuts at: 2329 2329
Size: 4568
MslI CAynn'nnrTG
Cuts at: 544 2557 2684 544
Size: 2013 127 2428
Fragments arranged by size:
2428 2013 127
MspA1I CmG'CkG
Cuts at: 139 417 598 657 839 1068 1250 1256 1464
Size: 278 181 59 182 229 182 6 208
Cuts at: 1464 1532 1679 1993 2353 3366 3611 139
Size: 68 147 314 360 1013 245 1096
Fragments arranged by size:
1096 1013 360 314 278 245 229 208
182 182 181 147 68 59 6
NaeI GCC'GGC
Cuts at: 1349 2749 1349
Size: 1400 3168
NarI GG'CG_CC
Cuts at: 1999 2247 1999
Size: 248 4320
NcoI C'CATG_G
Cuts at: 194 194
Size: 4568
NgoMIV G'CCGG_C
Cuts at: 1347 2747 1347
Size: 1400 3168
NspI r_CATG'y
Cuts at: 966 1344 1621 2121 3028 966
Size: 378 277 500 907 2506
Fragments arranged by size:
2506 907 500 378 277
PciI A'CATG_T
Cuts at: 3024 3024
Size: 4568
PfoI T'CCnGG_A
Cuts at: 1208 1208
Size: 4568
PmeI GTTT'AAAC
Cuts at: 1567 1567
Size: 4568
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 3707
Size: 32 4536
PpuMI rG'GwC_Cy
Cuts at: 603 603
Size: 4568
PspXI vC'TCGA_Gb
Cuts at: 880 880
Size: 4568
PstI C_TGCA'G
Cuts at: 704 981 1255 1615 2300 704
Size: 277 274 360 685 2972
Fragments arranged by size:
2972 685 360 277 274
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4568
PvuII CAG'CTG
Cuts at: 139 417 598 657 1068 1250 1256 1464 1532
Size: 278 181 59 411 182 6 208 68
Cuts at: 1532 1993 2353 139
Size: 461 360 2354
Fragments arranged by size:
2354 461 411 360 278 208 182 181
68 59 6
RsrII CG'GwC_CG
Cuts at: 2763 2763
Size: 4568
SacI G_AGCT'C
Cuts at: 1582 1582
Size: 4568
SalI G'TCGA_C
Cuts at: 1605 1605
Size: 4568
SapI GCTCTTCn'nnn_
Cuts at: 2591 2801 2591
Size: 210 4358
SbfI CC_TGCA'GG
Cuts at: 1615 1615
Size: 4568
ScaI AGT'ACT
Cuts at: 4085 4085
Size: 4568
SexAI A'CCwGG_T
Cuts at: 257 257
Size: 4568
SfcI C'TryA_G
Cuts at: 33 700 977 1251 1611 2296 3289 3480 33
Size: 667 277 274 360 685 993 191 1121
Fragments arranged by size:
1121 993 685 667 360 277 274 191
SfoI GGC'GCC
Cuts at: 2000 2248 2000
Size: 248 4320
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4568
SmaI CCC'GGG
Cuts at: 1590 1590
Size: 4568
SmlI C'TyrA_G
Cuts at: 112 234 880 1723 2638 3130 3392 112
Size: 122 646 843 915 492 262 1288
Fragments arranged by size:
1288 915 843 646 492 262 122
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4568
SphI G_CATG'C
Cuts at: 1621 2121 1621
Size: 500 4068
StuI AGG'CCT
Cuts at: 479 479
Size: 4568
StyI C'CwwG_G
Cuts at: 165 194 367 461 1481 1702 2056 165
Size: 29 173 94 1020 221 354 2677
Fragments arranged by size:
2677 1020 354 221 173 94 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 907 2151 2284 2743 2952 907
Size: 1244 133 459 209 2523
Fragments arranged by size:
2523 1244 459 209 133
TatI w'GTAC_w
Cuts at: 4083 4083
Size: 4568
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 395 427 557 589 4085 4117 395
Size: 32 130 32 3496 32 846
Fragments arranged by size:
3496 846 130 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2365 2365
Size: 4568
XbaI T'CTAG_A
Cuts at: 1380 1599 1380
Size: 219 4349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 192 2063 192
Size: 1871 2697
XhoI C'TCGA_G
Cuts at: 880 880
Size: 4568
XmaI C'CCGG_G
Cuts at: 1588 1588
Size: 4568
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BsrGI BssHII Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PsiI
PspOMI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XmnI ZraI