Restriction map for pF1KB8842
 (Linear) (Six-base) MAPSORT of: KIBB8842.seq  Check: 9146  from: 1  to: 1455



KIBB8842.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1181    1455 
   Size:       1181     274
 
AccI GT'mk_AC

Cuts at:      0     335     640    1455 
   Size:        335     305     815

  Fragments arranged by size: 

                 815     335     305
 
Acc65I G'GTAC_C

Cuts at:      0     808    1455 
   Size:        808     647
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     386    1455 
   Size:        386    1069
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     884    1455 
   Size:        884     571
 
AlwNI CAG_nnn'CTG

Cuts at:      0     897    1101    1455 
   Size:        897     204     354

  Fragments arranged by size: 

                 897     354     204
 
AvaI C'yCGr_G

Cuts at:      0     299     326     679     772    1455 
   Size:        299      27     353      93     683

  Fragments arranged by size: 

                 683     353     299      93      27
 
BamHI G'GATC_C

Cuts at:      0     441    1455 
   Size:        441    1014
 
BanI G'GyrC_C

Cuts at:      0     808    1455 
   Size:        808     647
 
BanII G_rGCy'C

Cuts at:      0     113    1455 
   Size:        113    1342
 
BbvCI CC'TCA_GC

Cuts at:      0     491     793    1455 
   Size:        491     302     662

  Fragments arranged by size: 

                 662     491     302
 
BglII A'GATC_T

Cuts at:      0     230    1455 
   Size:        230    1225
 
BlpI GC'TnA_GC

Cuts at:      0     609    1455 
   Size:        609     846
 
Bme1580I G_kGCm'C

Cuts at:      0     906    1209    1455 
   Size:        906     303     246

  Fragments arranged by size: 

                 906     303     246
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     162    1455 
   Size:        162    1293
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     705    1455 
   Size:        705     750
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331     514     650     753     973    1207    1455 
   Size:        331     183     136     103     220     234     248

  Fragments arranged by size: 

                 331     248     234     220     183     136     103
 
Bpu10I CC'TnA_GC

Cuts at:      0     491     649     793    1455 
   Size:        491     158     144     662

  Fragments arranged by size: 

                 662     491     158     144
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     111    1455 
   Size:         25      86    1344

  Fragments arranged by size: 

                1344      86      25
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1297    1455 
   Size:       1297     158
 
BsaWI w'CCGG_w

Cuts at:      0     245    1455 
   Size:        245    1210
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1357    1393    1455 
   Size:       1357      36      62

  Fragments arranged by size: 

                1357      62      36
 
BseYI C'CCAG_C

Cuts at:      0    1127    1455 
   Size:       1127     328
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     325     984    1141    1353    1455 
   Size:        130     195     659     157     212     102

  Fragments arranged by size: 

                 659     212     195     157     130     102
 
BsiEI CG_ry'CG

Cuts at:      0     711    1455 
   Size:        711     744
 
BsiHKAI G_wGCw'C

Cuts at:      0     601     610    1223    1455 
   Size:        601       9     613     232

  Fragments arranged by size: 

                 613     601     232       9
 
Bsp1286I G_dGCh'C

Cuts at:      0     113     601     610     906    1209    1223    1455 
   Size:        113     488       9     296     303      14     232

  Fragments arranged by size: 

                 488     303     296     232     113      14       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1181    1434    1455 
   Size:       1181     253      21

  Fragments arranged by size: 

                1181     253      21
 
BsrBI CCG'CTC

Cuts at:      0    1389    1455 
   Size:       1389      66
 
BsrFI r'CCGG_y

Cuts at:      0    1239    1455 
   Size:       1239     216
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     605    1261    1455 
   Size:        605     656     194

  Fragments arranged by size: 

                 656     605     194
 
BstEII G'GTnAC_C

Cuts at:      0     777    1455 
   Size:        777     678
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     438     805    1455 
   Size:        438     367     650

  Fragments arranged by size: 

                 650     438     367
 
BstYI r'GATC_y

Cuts at:      0     230     441    1097    1455 
   Size:        230     211     656     358

  Fragments arranged by size: 

                 656     358     230     211
 
BstZ17I GTA'TAC

Cuts at:      0     641    1455 
   Size:        641     814
 
BtgI C'CryG_G

Cuts at:      0      22      86     566    1455 
   Size:         22      64     480     889

  Fragments arranged by size: 

                 889     480      64      22
 
BtsI GCAGTG_nn'

Cuts at:      0     457    1331    1365    1455 
   Size:        457     874      34      90

  Fragments arranged by size: 

                 874     457      90      34
 
DraIII CAC_nnn'GTG

Cuts at:      0     605    1455 
   Size:        605     850
 
EaeI y'GGCC_r

Cuts at:      0    1415    1455 
   Size:       1415      40
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331     386     514     650     753     973    1207    1455 
   Size:        331      55     128     136     103     220     234     248

  Fragments arranged by size: 

                 331     248     234     220     136     128     103      55
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     118    1339    1455 
   Size:        118    1221     116

  Fragments arranged by size: 

                1221     118     116
 
EcoO109I rG'GnC_Cy

Cuts at:      0     495     894    1164    1455 
   Size:        495     399     270     291

  Fragments arranged by size: 

                 495     399     291     270
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     900    1455 
   Size:        900     555
 
HincII GTy'rAC

Cuts at:      0     156    1455 
   Size:        156    1299
 
HpaI GTT'AAC

Cuts at:      0     156    1455 
   Size:        156    1299
 
KpnI G_GTAC'C

Cuts at:      0     812    1455 
   Size:        812     643
 
MslI CAynn'nnrTG

Cuts at:      0     436    1455 
   Size:        436    1019
 
MspA1I CmG'CkG

Cuts at:      0      31     309     490     549     731     960    1142    1148 
   Size:         31     278     181      59     182     229     182       6

Cuts at:   1148    1356    1424    1455 
   Size:        208      68      31

  Fragments arranged by size: 

                 278     229     208     182     182     181      68      59
                  31      31       6
 
NaeI GCC'GGC

Cuts at:      0    1241    1455 
   Size:       1241     214
 
NcoI C'CATG_G

Cuts at:      0      86    1455 
   Size:         86    1369
 
NgoMIV G'CCGG_C

Cuts at:      0    1239    1455 
   Size:       1239     216
 
NspI r_CATG'y

Cuts at:      0     858    1236    1455 
   Size:        858     378     219

  Fragments arranged by size: 

                 858     378     219
 
PfoI T'CCnGG_A

Cuts at:      0    1100    1455 
   Size:       1100     355
 
PpuMI rG'GwC_Cy

Cuts at:      0     495    1455 
   Size:        495     960
 
PspXI vC'TCGA_Gb

Cuts at:      0     772    1455 
   Size:        772     683
 
PstI C_TGCA'G

Cuts at:      0     596     873    1147    1455 
   Size:        596     277     274     308

  Fragments arranged by size: 

                 596     308     277     274
 
PvuII CAG'CTG

Cuts at:      0      31     309     490     549     960    1142    1148    1356 
   Size:         31     278     181      59     411     182       6     208

Cuts at:   1356    1424    1455 
   Size:         68      31

  Fragments arranged by size: 

                 411     278     208     182     181      68      59      31
                  31       6
 
SexAI A'CCwGG_T

Cuts at:      0     149    1455 
   Size:        149    1306
 
SfcI C'TryA_G

Cuts at:      0     592     869    1143    1455 
   Size:        592     277     274     312

  Fragments arranged by size: 

                 592     312     277     274
 
SmlI C'TyrA_G

Cuts at:      0       4     126     772    1455 
   Size:          4     122     646     683

  Fragments arranged by size: 

                 683     646     122       4
 
StuI AGG'CCT

Cuts at:      0     371    1455 
   Size:        371    1084
 
StyI C'CwwG_G

Cuts at:      0      57      86     259     353    1373    1455 
   Size:         57      29     173      94    1020      82

  Fragments arranged by size: 

                1020     173      94      82      57      29
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     799    1455 
   Size:        799     656
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     287     319     449     481    1455 
   Size:        287      32     130      32     974

  Fragments arranged by size: 

                 974     287     130      32      32
 
XbaI T'CTAG_A

Cuts at:      0    1272    1455 
   Size:       1272     183
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      84    1455 
   Size:         84    1371
 
XhoI C'TCGA_G

Cuts at:      0     772    1455 
   Size:        772     683
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AlfI    AlwNI     AvaI    BamHI
     BanI    BanII    BbvCI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaWI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrFI   BstAPI   BstEII
    BstXI    BstYI  BstZ17I     BtgI     BtsI   DraIII     EaeI  Eco57MI
    EcoNI EcoO109I     FalI   HincII     HpaI     KpnI     MslI   MspA1I
     NaeI     NcoI   NgoMIV     NspI     PfoI    PpuMI    PspXI     PstI
    PvuII    SexAI     SfcI     SmlI     StuI     StyI    TaqII     TstI
     XbaI     XcmI     XhoI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI     AleI
     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI
    BmgBI     BmtI    BsaAI    BsaBI    BsaHI    BsaXI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BssHII    BssSI    BstBI
   Bsu36I    BtgZI     ClaI    CspCI     DraI     DrdI     EagI     EarI
     EciI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
    Hin4I  HindIII     KasI     MfeI     MluI     MmeI     MscI     NarI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     TatI  Tth111I     XmaI     XmnI
     ZraI
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