Restriction map for pF1KB8818
##################################
# NO CUT ENZYMES (  171 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BclI        BcnI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsoBI       
Bsp1286I    BspDI       BspEI       BspHI       BspMI       
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
Cfr13I      ClaI        CpoI        DraII       DrdI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HapII       
HgaI        Hin1I       HindIII     HpaII       KasI        
KpnI        Ksp632I     KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MspI        
MunI        MvnI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NlaIV       NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        Tsp45I      Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AciI           97  CCGC                 CCGC(-3/-1)
AflII         580  CTTAAG               C^TTAAG
AflIII        410  ACATGT               A^C[GA][CT]GT
AlwNI          39  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI          466  ATTAAT               AT^TAAT
AsnI          466  ATTAAT               AT^TAAT
Asp700I       907  GAAATTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvI           41  GCAGC                GCAGC(8/12)
BciVI        1084  GGATAC               GTATCC(6/5)
BfrI          580  CTTAAG               C^TTAAG
BsaJI         140  CCATGG               C^C[ACGT][ACGT]GG
BseRI          54  CTCCTC               GAGGAG(10/8)
BsiYI         766  CCTAATTCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          766  CCTAATTCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI           65  GAATGC               GAATGC(1/-1)
Bsp1407I      413  TGTACA               T^GTACA
BspLU11I      410  ACATGT               A^CATGT
BsrDI         291  CATTGC               GCAATG(2/0)
BsrGI         413  TGTACA               T^GTACA
BssKI        1081  CCAGG                ^CC[ACGT]GG
BstNI        1081  CCAGG                CC^[AT]GG
BstYI         966  AGATCC               [GA]^GATC[CT]
CfoI           69  GCGC                 GCG^C
DraI         1075  TTTAAA               TTT^AAA
DsaI          140  CCATGG               C^C[GA][CT]GG
EcoRII       1081  CCAGG                ^CC[AT]GG
EcoT14I       140  CCATGG               C^C[AT][AT]GG
Fnu4HI         41  GCAGC                GC^[ACGT]GC
HaeIII         47  GGCC                 GG^CC
HhaI           69  GCGC                 GCG^C
HinP1I         69  GCGC                 G^CGC
HincII        168  GTTAAC               GT[CT]^[GA]AC
HindII        168  GTTAAC               GT[CT]^[GA]AC
HpaI          168  GTTAAC               GTT^AAC
ItaI           41  GCAGC                GC^[ACGT]GC
MaeII         284  ACGT                 A^CGT
MflI          966  AGATCC               [GA]^GATC[CT]
MspA1I         42  CAGCTG               C[AC]G^C[GT]G
MvaI         1081  CCAGG                CC^[AT]GG
MwoI          784  GCCACTACAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          140  CCATGG               C^CATGG
NspI          410  ACATGT               [GA]CATG^[CT]
PshBI         466  ATTAAT               AT^TAAT
PvuII          42  CAGCTG               CAG^CTG
ScaI         1138  AGTACT               AGT^ACT
ScrFI        1081  CCAGG                CC^[ACGT]GG
SfaNI         537  GATGC                GCATC(5/9)
SfcI          788  CTACAG               C^T[GA][CT]AG
SmlI          580  CTTAAG               C^T[CT][GA]AG
SspBI         413  TGTACA               T^GTACA
StyI          140  CCATGG               C^C[AT][AT]GG
TaqI          855  TCGA                 T^CGA
TseI           41  GCAGC                G^C[AT]GC
TthHB8I       855  TCGA                 T^CGA
XhoII         966  AGATCC               [GA]^GATC[CT]
XmnI          907  GAAATTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AlwI          967  GATCC                GGATC(4/5)
              450  GGATC               
BfaI          364  CTAG                 C^TAG
             1127  CTAG                
BsmFI         201  GGGAC                GGGAC(10/14)
              137  GTCCC               
Cac8I          44  GCTGGC               GC[ACGT]^[ACGT]GC
              540  GCTTGC              
DdeI          111  CTCAG                C^T[ACGT]AG
              963  CTTAG               
DraIII        362  CACTAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
              603  CACAATGTG           
HphI         1042  GGTGA                GGTGA(8/7)
              246  TCACC               
MaeI          364  CTAG                 C^TAG
             1127  CTAG                
MslI           15  CATAACAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              663  CATTCTTGTG          
TspRI         395  CACTG                CA[GC]TG[ACGT][ACGT]^
             1112  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BsrI        
BstF5I      DpnII       FokI        HinfI       MaeIII      
MboI        MboII       MnlI        MseI        NdeII       
NlaIII      RsaI        Sau3AI      TfiI        Tru9I       
Tsp509I     
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