Restriction map for pF1KB8811
 (Linear) (Six-base) MAPSORT of: KIBB8811.seq  Check: 8800  from: 1  to: 1086



KIBB8811.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AccI GT'mk_AC

Cuts at:      0     161    1086 
   Size:        161     925
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1084    1086 
   Size:       1084       2
 
AflIII A'CryG_T

Cuts at:      0      11    1086 
   Size:         11    1075
 
AvaI C'yCGr_G

Cuts at:      0     259    1086 
   Size:        259     827
 
BamHI G'GATC_C

Cuts at:      0     248    1086 
   Size:        248     838
 
BanI G'GyrC_C

Cuts at:      0     439     450     874    1086 
   Size:        439      11     424     212

  Fragments arranged by size: 

                 439     424     212      11
 
BanII G_rGCy'C

Cuts at:      0     415     473    1086 
   Size:        415      58     613

  Fragments arranged by size: 

                 613     415      58
 
BbeI G_GCGC'C

Cuts at:      0     443     454     878    1086 
   Size:        443      11     424     208

  Fragments arranged by size: 

                 443     424     208      11
 
BbvCI CC'TCA_GC

Cuts at:      0     175     679    1086 
   Size:        175     504     407

  Fragments arranged by size: 

                 504     407     175
 
BglI GCCn_nnn'nGGC

Cuts at:      0     448    1086 
   Size:        448     638
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     988    1086 
   Size:        988      98
 
Bpu10I CC'TnA_GC

Cuts at:      0     175     679    1086 
   Size:        175     504     407

  Fragments arranged by size: 

                 504     407     175
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     473     964    1086 
   Size:        473     491     122

  Fragments arranged by size: 

                 491     473     122
 
BsaAI yAC'GTr

Cuts at:      0      14      30     723    1086 
   Size:         14      16     693     363

  Fragments arranged by size: 

                 693     363      16      14
 
BsaHI Gr'CG_yC

Cuts at:      0     158     440     451     875    1086 
   Size:        158     282      11     424     211

  Fragments arranged by size: 

                 424     282     211     158      11
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     796    1086 
   Size:        796     290
 
BseYI C'CCAG_C

Cuts at:      0     153     435    1022    1086 
   Size:        153     282     587      64

  Fragments arranged by size: 

                 587     282     153      64
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     186     217     886    1078    1086 
   Size:        186      31     669     192       8

  Fragments arranged by size: 

                 669     192     186      31       8
 
BsiHKAI G_wGCw'C

Cuts at:      0     415     473     728    1086 
   Size:        415      58     255     358

  Fragments arranged by size: 

                 415     358     255      58
 
Bsp1286I G_dGCh'C

Cuts at:      0     415     473     728    1086 
   Size:        415      58     255     358

  Fragments arranged by size: 

                 415     358     255      58
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     197     917    1086 
   Size:        197     720     169

  Fragments arranged by size: 

                 720     197     169
 
BsrGI T'GTAC_A

Cuts at:      0     194    1086 
   Size:        194     892
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     909    1086 
   Size:        909     177
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     108     435     699     753    1086 
   Size:        108     327     264      54     333

  Fragments arranged by size: 

                 333     327     264     108      54
 
BstYI r'GATC_y

Cuts at:      0     248    1086 
   Size:        248     838
 
BtgI C'CryG_G

Cuts at:      0     252     401     416     428     518     577    1086 
   Size:        252     149      15      12      90      59     509

  Fragments arranged by size: 

                 509     252     149      90      59      15      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     165     297     672     899    1086 
   Size:        165     132     375     227     187

  Fragments arranged by size: 

                 375     227     187     165     132
 
EaeI y'GGCC_r

Cuts at:      0     404     890     923     947    1028    1086 
   Size:        404     486      33      24      81      58

  Fragments arranged by size: 

                 486     404      81      58      33      24
 
EarI CTCTTCn'nnn_

Cuts at:      0     478     577    1086 
   Size:        478      99     509

  Fragments arranged by size: 

                 509     478      99
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      47     856    1025    1086 
   Size:         47     809     169      61

  Fragments arranged by size: 

                 809     169      61      47
 
EcoICRI GAG'CTC

Cuts at:      0     413     471    1086 
   Size:        413      58     615

  Fragments arranged by size: 

                 615     413      58
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     988    1084    1086 
   Size:        988      96       2

  Fragments arranged by size: 

                 988      96       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     987    1086 
   Size:        987      99
 
EcoO109I rG'GnC_Cy

Cuts at:      0     263     897    1086 
   Size:        263     634     189

  Fragments arranged by size: 

                 634     263     189
 
HaeII r_GCGC'y

Cuts at:      0     443     454     586     648     746     878    1086 
   Size:        443      11     132      62      98     132     208

  Fragments arranged by size: 

                 443     208     132     132      98      62      11
 
HincII GTy'rAC

Cuts at:      0     867    1086 
   Size:        867     219
 
KasI G'GCGC_C

Cuts at:      0     439     450     874    1086 
   Size:        439      11     424     212

  Fragments arranged by size: 

                 439     424     212      11
 
MscI TGG'CCA

Cuts at:      0     892     925     949    1086 
   Size:        892      33      24     137

  Fragments arranged by size: 

                 892     137      33      24
 
MslI CAynn'nnrTG

Cuts at:      0      85     334    1086 
   Size:         85     249     752

  Fragments arranged by size: 

                 752     249      85
 
MspA1I CmG'CkG

Cuts at:      0     213    1022    1062    1086 
   Size:        213     809      40      24

  Fragments arranged by size: 

                 809     213      40      24
 
NarI GG'CG_CC

Cuts at:      0     440     451     875    1086 
   Size:        440      11     424     211

  Fragments arranged by size: 

                 440     424     211      11
 
NcoI C'CATG_G

Cuts at:      0     518    1086 
   Size:        518     568
 
PasI CC'CwG_GG

Cuts at:      0     764    1044    1086 
   Size:        764     280      42

  Fragments arranged by size: 

                 764     280      42
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     945    1086 
   Size:        945     141
 
PmlI CAC'GTG

Cuts at:      0      14      30     723    1086 
   Size:         14      16     693     363

  Fragments arranged by size: 

                 693     363      16      14
 
PpuMI rG'GwC_Cy

Cuts at:      0     263    1086 
   Size:        263     823
 
PvuII CAG'CTG

Cuts at:      0    1022    1062    1086 
   Size:       1022      40      24

  Fragments arranged by size: 

                1022      40      24
 
SacI G_AGCT'C

Cuts at:      0     415     473    1086 
   Size:        415      58     613

  Fragments arranged by size: 

                 613     415      58
 
SapI GCTCTTCn'nnn_

Cuts at:      0     478    1086 
   Size:        478     608
 
SexAI A'CCwGG_T

Cuts at:      0    1052    1086 
   Size:       1052      34
 
SfoI GGC'GCC

Cuts at:      0     441     452     876    1086 
   Size:        441      11     424     210

  Fragments arranged by size: 

                 441     424     210      11
 
SmaI CCC'GGG

Cuts at:      0     261    1086 
   Size:        261     825
 
StyI C'CwwG_G

Cuts at:      0     518     893    1086 
   Size:        518     375     193

  Fragments arranged by size: 

                 518     375     193
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     207     377     519    1086 
   Size:        207     170     142     567

  Fragments arranged by size: 

                 567     207     170     142
 
TatI w'GTAC_w

Cuts at:      0     194    1086 
   Size:        194     892
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     105    1086 
   Size:        105     981
 
XmaI C'CCGG_G

Cuts at:      0     259    1086 
   Size:        259     827
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AvaI    BamHI     BanI    BanII     BbeI
    BbvCI     BglI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BseRI
    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrGI   BstAPI    BstXI
    BstYI     BtgI    BtgZI     EaeI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    HaeII   HincII     KasI     MscI     MslI   MspA1I
     NarI     NcoI     PasI    PflMI     PmlI    PpuMI    PvuII     SacI
     SapI    SexAI     SfoI     SmaI     StyI    TaqII     TatI     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI     BbsI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
    BpuEI    BsaBI    BsaWI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI   BssHII    BssSI    BstBI
   BstEII  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     NaeI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI     PfoI     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI     SbfI
     ScaI     SfcI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI
     XhoI     XmnI     ZraI
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