Restriction map for pF1KB8810
##################################
# NO CUT ENZYMES (  161 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        AlwI        Aor51HI     ApaI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbvCI       BcgI        
BclI        BcnI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmAI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspEI       BspHI       BspLU11I    BsrBI       BsrFI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Cac8I       CelII       Cfr10I      CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO109I    
EcoO65I     EcoRI       EcoRV       FauI        FbaI        
FseI        FspI        HaeII       HapII       HgaI        
Hin1I       HincII      HindII      HpaI        HpaII       
KasI        KpnI        KspI        MamI        MluI        
MluNI       MroI        MscI        MspA1I      MspI        
MvnI        NaeI        NarI        NciI        NcoI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmeI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

AfaI          616  GTAC                 GT^AC
AluI          812  AGCT                 AG^CT
Alw44I        400  GTGCAC               G^TGCAC
AlwNI        1010  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI           46  CCTCAGG              CC^T[ACGT]AGG
ApaLI         400  GTGCAC               G^TGCAC
AspHI         400  GTGCAC               G[AT]GC[AT]^C
AvaII         909  GGACC                G^G[AT]CC
BanII         133  GGGCTC               G[GA]GC[CT]^C
BbrPI        1028  CACGTG               CAC^GTG
BciVI         549  GTATCC               GTATCC(6/5)
BglII         653  AGATCT               A^GATCT
BpmI          288  CTGGAG               CTGGAG(16/14)
BsaAI        1028  CACGTG               [CT]AC^GT[GA]
BsgI          868  CTGCAC               GTGCAG(16/14)
BsiHKAI       400  GTGCAC               G[AT]GC[AT]^C
BsiYI         305  CCTTGTGTAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          305  CCTTGTGTAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspDI         987  ATCGAT               AT^CGAT
BsrI          287  ACTGG                ACTGG(1/-1)
BssSI         136  CTCGTG               CACGAG(-5/-1)
BstF5I        962  GGATG                GGATG(2/0)
BstYI         653  AGATCT               [GA]^GATC[CT]
Bsu36I         46  CCTCAGG              CC^T[ACGT]AGG
CfoI          322  GCGC                 GCG^C
Cfr13I        909  GGACC                G^G[ACGT]CC
ClaI          987  ATCGAT               AT^CGAT
DpnII         654  GATC                 ^GATC
EaeI           86  CGGCCA               [CT]^GGCC[GA]
Eco81I         46  CCTCAGG              CC^T[ACGT]AGG
EcoNI         305  CCTTGTGTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       155  CCTTGG               C^C[AT][AT]GG
EcoT22I       540  ATGCAT               ATGCA^T
FokI          962  GGATG                GGATG(9/13)
HaeIII         87  GGCC                 GG^CC
HhaI          322  GCGC                 GCG^C
HinP1I        322  GCGC                 G^CGC
HindIII       811  AAGCTT               A^AGCTT
HphI          219  GGTGA                GGTGA(8/7)
MaeII        1029  ACGT                 A^CGT
MaeIII         62  GTGAC                ^GT[ACGT]AC
MboI          654  GATC                 ^GATC
MfeI          897  CAATTG               C^AATTG
MflI          653  AGATCT               [GA]^GATC[CT]
MseI          806  TTAA                 T^TAA
MslI          390  CATTGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          897  CAATTG               C^AATTG
NdeII         654  GATC                 ^GATC
NlaIV         909  GGACCC               GG[ACGT]^[ACGT]CC
NsiI          540  ATGCAT               ATGCA^T
PmaCI        1028  CACGTG               CAC^GTG
PmlI         1028  CACGTG               CAC^GTG
RsaI          616  GTAC                 GT^AC
Sau3AI        654  GATC                 ^GATC
Sau96I        909  GGACC                G^G[ACGT]CC
StyI          155  CCTTGG               C^C[AT][AT]GG
TaqI          988  TCGA                 T^CGA
Tru9I         806  TTAA                 T^TAA
Tsp45I         62  GTGAC                ^GT[GC]AC
TthHB8I       988  TCGA                 T^CGA
XhoII         653  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AcsI          213  AAATTT               [GA]^AATT[CT]
             1023  AAATTC              
ApoI          213  AAATTT               [GA]^AATT[CT]
             1023  AAATTC              
BbsI          121  GTCTTC               GAAGAC(2/6)
             1068  GTCTTC              
BbvI          659  GCTGC                GCAGC(8/12)
              902  GCTGC               
BmyI          133  GGGCTC               G[AGT]GC[ACT]^C
              400  GTGCAC              
BpuAI         121  GTCTTC               GAAGAC(2/6)
             1068  GTCTTC              
BsaJI         155  CCTTGG               C^C[ACGT][ACGT]GG
              580  CCCTGG              
BseRI          44  CTCCTC               GAGGAG(10/8)
              496  CTCCTC              
Bsp1286I      133  GGGCTC               G[AGT]GC[ACT]^C
              400  GTGCAC              
BspMI         367  ACCTGC               ACCTGC(4/8)
              349  GCAGGT              
BsrDI         390  CATTGC               GCAATG(2/0)
              274  GCAATG              
EarI          575  CTCTTC               CTCTTC(1/4)
              667  CTCTTC              
Fnu4HI        659  GCTGC                GC^[ACGT]GC
              902  GCTGC               
ItaI          659  GCTGC                GC^[ACGT]GC
              902  GCTGC               
Ksp632I       575  CTCTTC               CTCTTC(1/4)
              667  CTCTTC              
MwoI          265  GCCTACTATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              966  GCTGAAACGGC         
NdeI          344  CATATG               CA^TATG
              799  CATATG              
SfaNI         301  GATGC                GCATC(5/9)
              963  GATGC               
TseI          659  GCTGC                G^C[AT]GC
              902  GCTGC               
TspRI         696  CACTG                CA[GC]TG[ACGT][ACGT]^
              981  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   16 enzymes) #
#########################################

AciI        BfaI        BssKI       BstNI       DdeI        
EcoRII      HinfI       MaeI        MboII       MnlI        
MvaI        NlaIII      NspI        ScrFI       TfiI        
Tsp509I     
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