Restriction map for pF1KB8810
 (Linear) (Six-base) MAPSORT of: KIBB8810.seq  Check: 8879  from: 1  to: 1083



KIBB8810.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     340    1083 
   Size:        340     743
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     654    1037    1083 
   Size:        654     383      46

  Fragments arranged by size: 

                 654     383      46
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1015    1083 
   Size:       1015      68
 
ApaLI G'TGCA_C

Cuts at:      0     400    1083 
   Size:        400     683
 
ApoI r'AATT_y

Cuts at:      0     213    1023    1083 
   Size:        213     810      60

  Fragments arranged by size: 

                 810     213      60
 
BanII G_rGCy'C

Cuts at:      0     137    1083 
   Size:        137     946
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     114    1061    1083 
   Size:        114     947      22

  Fragments arranged by size: 

                 947     114      22
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     560    1083 
   Size:        560     523
 
BfrBI ATG'CAT

Cuts at:      0     542    1083 
   Size:        542     541
 
BglII A'GATC_T

Cuts at:      0     653    1083 
   Size:        653     430
 
Bme1580I G_kGCm'C

Cuts at:      0     404    1083 
   Size:        404     679
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     309    1083 
   Size:        309     774
 
BsaAI yAC'GTr

Cuts at:      0    1030    1083 
   Size:       1030      53
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     479     509    1083 
   Size:        479      30     574

  Fragments arranged by size: 

                 574     479      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      35     487    1083 
   Size:         35     452     596

  Fragments arranged by size: 

                 596     452      35
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     853    1083 
   Size:        853     230
 
BsiHKAI G_wGCw'C

Cuts at:      0     404    1083 
   Size:        404     679
 
Bsp1286I G_dGCh'C

Cuts at:      0     137     404    1083 
   Size:        137     267     679

  Fragments arranged by size: 

                 679     267     137
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     340     376    1083 
   Size:        340      36     707

  Fragments arranged by size: 

                 707     340      36
 
BsrDI GCAATG_nn'

Cuts at:      0     281     389    1083 
   Size:        281     108     694

  Fragments arranged by size: 

                 694     281     108
 
BssSI C'ACGA_G

Cuts at:      0     136    1083 
   Size:        136     947
 
BstYI r'GATC_y

Cuts at:      0     653    1083 
   Size:        653     430
 
Bsu36I CC'TnA_GG

Cuts at:      0      47    1083 
   Size:         47    1036
 
ClaI AT'CG_AT

Cuts at:      0     988    1083 
   Size:        988      95
 
EaeI y'GGCC_r

Cuts at:      0      86    1083 
   Size:         86     997
 
EarI CTCTTCn'nnn_

Cuts at:      0     581     673    1083 
   Size:        581      92     410

  Fragments arranged by size: 

                 581     410      92
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      20    1083 
   Size:         20    1063
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     309     654    1037    1083 
   Size:        309     345     383      46

  Fragments arranged by size: 

                 383     345     309      46
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     309    1083 
   Size:        309     774
 
HindIII A'AGCT_T

Cuts at:      0     811    1083 
   Size:        811     272
 
MfeI C'AATT_G

Cuts at:      0     897    1083 
   Size:        897     186
 
MslI CAynn'nnrTG

Cuts at:      0     394    1083 
   Size:        394     689
 
NdeI CA'TA_TG

Cuts at:      0     345     800    1083 
   Size:        345     455     283

  Fragments arranged by size: 

                 455     345     283
 
NsiI A_TGCA'T

Cuts at:      0     544    1083 
   Size:        544     539
 
NspI r_CATG'y

Cuts at:      0     442     542     602     935    1083 
   Size:        442     100      60     333     148

  Fragments arranged by size: 

                 442     333     148     100      60
 
PfoI T'CCnGG_A

Cuts at:      0     830    1083 
   Size:        830     253
 
PmlI CAC'GTG

Cuts at:      0    1030    1083 
   Size:       1030      53
 
PsiI TTA'TAA

Cuts at:      0     628    1083 
   Size:        628     455
 
StyI C'CwwG_G

Cuts at:      0     155    1083 
   Size:        155     928
 
TatI w'GTAC_w

Cuts at:      0     615    1083 
   Size:        615     468
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     479     511    1083 
   Size:        479      32     572

  Fragments arranged by size: 

                 572     479      32
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI    ApaLI     ApoI    BanII     BbsI    BciVI
    BfrBI    BglII Bme1580I     BpmI    BsaAI    BsaXI    BseRI     BsgI
  BsiHKAI Bsp1286I    BspMI    BsrDI    BssSI    BstYI   Bsu36I     ClaI
     EaeI     EarI     EciI  Eco57MI    EcoNI  HindIII     MfeI     MslI
     NdeI     NsiI     NspI     PfoI     PmlI     PsiI     StyI     TatI
     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
     BclI     BglI     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BseYI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII
   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     BtsI
    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
     HpaI     KasI     KpnI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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