Restriction map for pF1KB8807
 (Linear) (Six-base) MAPSORT of: KIBB8807.seq  Check: 5656  from: 1  to: 1065



KIBB8807.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

Acc65I G'GTAC_C

Cuts at:      0      34     392    1065 
   Size:         34     358     673

  Fragments arranged by size: 

                 673     358      34
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     542    1065 
   Size:        542     523
 
ApoI r'AATT_y

Cuts at:      0     502    1065 
   Size:        502     563
 
BamHI G'GATC_C

Cuts at:      0     269    1065 
   Size:        269     796
 
BanI G'GyrC_C

Cuts at:      0      34     392     683    1065 
   Size:         34     358     291     382

  Fragments arranged by size: 

                 382     358     291      34
 
BanII G_rGCy'C

Cuts at:      0     432    1065 
   Size:        432     633
 
BbvCI CC'TCA_GC

Cuts at:      0     679    1065 
   Size:        679     386
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     561    1065 
   Size:        561     504
 
BglI GCCn_nnn'nGGC

Cuts at:      0     149    1065 
   Size:        149     916
 
BglII A'GATC_T

Cuts at:      0     359    1065 
   Size:        359     706
 
Bme1580I G_kGCm'C

Cuts at:      0     113     688    1065 
   Size:        113     575     377

  Fragments arranged by size: 

                 575     377     113
 
BmgBI CAC'GTC

Cuts at:      0      57    1065 
   Size:         57    1008
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      96     303     683    1065 
   Size:         96     207     380     382

  Fragments arranged by size: 

                 382     380     207      96
 
BmtI G_CTAG'C

Cuts at:      0     490    1065 
   Size:        490     575
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     603    1065 
   Size:        603     462
 
Bpu10I CC'TnA_GC

Cuts at:      0     679    1065 
   Size:        679     386
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      19    1065 
   Size:         19    1046
 
BsaWI w'CCGG_w

Cuts at:      0     923    1065 
   Size:        923     142
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     313    1065 
   Size:        313     752
 
BsiHKAI G_wGCw'C

Cuts at:      0      93     555    1065 
   Size:         93     462     510

  Fragments arranged by size: 

                 510     462      93
 
Bsp1286I G_dGCh'C

Cuts at:      0      93     113     432     555     688    1065 
   Size:         93      20     319     123     133     377

  Fragments arranged by size: 

                 377     319     133     123      93      20
 
BspEI T'CCGG_A

Cuts at:      0     923    1065 
   Size:        923     142
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     224     248     941    1065 
   Size:        224      24     693     124

  Fragments arranged by size: 

                 693     224     124      24
 
BsrBI CCG'CTC

Cuts at:      0     822    1065 
   Size:        822     243
 
BsrDI GCAATG_nn'

Cuts at:      0     159    1065 
   Size:        159     906
 
BsrGI T'GTAC_A

Cuts at:      0     350    1065 
   Size:        350     715
 
BstYI r'GATC_y

Cuts at:      0     269     359    1065 
   Size:        269      90     706

  Fragments arranged by size: 

                 706     269      90
 
BtgI C'CryG_G

Cuts at:      0     306    1065 
   Size:        306     759
 
BtsI GCAGTG_nn'

Cuts at:      0     488     555     876    1065 
   Size:        488      67     321     189

  Fragments arranged by size: 

                 488     321     189      67
 
EaeI y'GGCC_r

Cuts at:      0     227     303     398     644    1065 
   Size:        227      76      95     246     421

  Fragments arranged by size: 

                 421     246     227      95      76
 
EarI CTCTTCn'nnn_

Cuts at:      0     250     500     518     530     789    1065 
   Size:        250     250      18      12     259     276

  Fragments arranged by size: 

                 276     259     250     250      18      12
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     542     603    1065 
   Size:        542      61     462

  Fragments arranged by size: 

                 542     462      61
 
EcoO109I rG'GnC_Cy

Cuts at:      0     474    1065 
   Size:        474     591
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     559    1065 
   Size:        559     506
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     816     848    1065 
   Size:        816      32     217

  Fragments arranged by size: 

                 816     217      32
 
KpnI G_GTAC'C

Cuts at:      0      38     396    1065 
   Size:         38     358     669

  Fragments arranged by size: 

                 669     358      38
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     891    1065 
   Size:        891     174
 
MscI TGG'CCA

Cuts at:      0     229     305     400     646    1065 
   Size:        229      76      95     246     419

  Fragments arranged by size: 

                 419     246     229      95      76
 
MslI CAynn'nnrTG

Cuts at:      0     736     850    1065 
   Size:        736     114     215

  Fragments arranged by size: 

                 736     215     114
 
MspA1I CmG'CkG

Cuts at:      0     117    1065 
   Size:        117     948
 
NcoI C'CATG_G

Cuts at:      0     306    1065 
   Size:        306     759
 
NheI G'CTAG_C

Cuts at:      0     486    1065 
   Size:        486     579
 
NspI r_CATG'y

Cuts at:      0     315    1065 
   Size:        315     750
 
SapI GCTCTTCn'nnn_

Cuts at:      0     250     500    1065 
   Size:        250     250     565

  Fragments arranged by size: 

                 565     250     250
 
SexAI A'CCwGG_T

Cuts at:      0     839    1065 
   Size:        839     226
 
StyI C'CwwG_G

Cuts at:      0     306    1065 
   Size:        306     759
 
TatI w'GTAC_w

Cuts at:      0     119     350     701    1065 
   Size:        119     231     351     364

  Fragments arranged by size: 

                 364     351     231     119
 
Tth111I GACn'n_nGTC

Cuts at:      0     841    1065 
   Size:        841     224
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      93    1065 
   Size:         93     972
 

 Enzymes that do cut:

   Acc65I     AcuI     ApoI    BamHI     BanI    BanII    BbvCI    BciVI
     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I
     BsaI    BsaWI    BseRI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrBI
    BsrDI    BsrGI    BstYI     BtgI     BtsI     EaeI     EarI  Eco57MI
 EcoO109I     FalI    Hin4I     KpnI     MmeI     MscI     MslI   MspA1I
     NcoI     NheI     NspI     SapI    SexAI     StyI     TatI  Tth111I
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BbeI     BbsI     BcgI
     BclI    BfrBI     BlpI     BplI    BpuEI    BsaAI    BsaBI    BsaHI
    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspHI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     MfeI     MluI     NaeI
     NarI     NdeI   NgoMIV     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI     XbaI     XhoI
     XmaI     XmnI     ZraI
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