Restriction map for pF1KB8805
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AhdI        Alw44I      AlwI        
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvrII       BamHI       BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BpuAI       BsaAI       BsaBI       
BsaI        BseAI       BsgI        BsiWI       BsmAI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrDI       BsrGI       
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     Bsu36I      ClaI        CpoI        
DpnII       DraI        DraIII      Eam1105I    Eco47III    
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        HindIII     HpaI        
KpnI        MaeI        MamI        MboI        MfeI        
MflI        MluI        MroI        MseI        MslI        
MunI        NcoI        NdeI        NdeII       NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SalI        Sau3AI      SbfI        ScaI        SfcI        
SfiI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TfiI        Tru9I       
Tsp45I      Tsp509I     Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       


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# ONE CUT ENZYMES (   20 enzymes) #
###################################

AgeI          217  ACCGGT               A^CCGGT
AlwNI          79  CAGCCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       939  GAACCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII         761  TGCGCA               TGC^GCA
BalI          246  TGGCCA               TGG^CCA
BsaWI         217  ACCGGT               [AT]^CCGG[AT]
BsmFI         378  GTCCC                GGGAC(10/14)
BstAPI         78  GCAGCCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI          601  GACTACCACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI          462  GGCCGGCC             GGCCGG^CC
FspI          761  TGCGCA               TGC^GCA
MaeIII        221  GTAAC                ^GT[ACGT]AC
MluNI         246  TGGCCA               TGG^CCA
MscI          246  TGGCCA               TGG^CCA
NlaIII       1002  CATG                 CATG^
PinAI         217  ACCGGT               A^CCGGT
PvuII         394  CAGCTG               CAG^CTG
SexAI         962  ACCTGGT              A^CC[AT]GGT
TspRI         320  CAGTG                CA[GC]TG[ACGT][ACGT]^
XmnI          939  GAACCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccI          697  GTCTAC               GT^[AC][GT]AC
             1012  GTCTAC              
ApaI          315  GGGCCC               GGGCC^C
              550  GGGCCC              
AspHI         304  GAGCTC               G[AT]GC[AT]^C
              703  GAGCTC              
AvaII         596  GGACC                G^G[AT]CC
             1029  GGACC               
BlpI          396  GCTGAGC              GC^T[ACGT]AGC
              705  GCTCAGC             
BpmI            6  CTGGAG               CTGGAG(16/14)
              187  CTGGAG              
Bpu1102I      396  GCTGAGC              GC^T[ACGT]AGC
              705  GCTCAGC             
BsiHKAI       304  GAGCTC               G[AT]GC[AT]^C
              703  GAGCTC              
BssSI         276  CTCGTG               CACGAG(-5/-1)
              921  CTCGTG              
CelII         396  GCTGAGC              GC^T[ACGT]AGC
              705  GCTCAGC             
DraII         314  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              550  GGGCCCT             
EagI          461  CGGCCG               C^GGCCG
              824  CGGCCG              
EclXI         461  CGGCCG               C^GGCCG
              824  CGGCCG              
Eco52I        461  CGGCCG               C^GGCCG
              824  CGGCCG              
EcoO109I      314  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              550  GGGCCCT             
HgaI          388  GACGC                GACGC(5/10)
              851  GCGTC               
HinfI         404  GACTC                G^A[ACGT]TC
              514  GACTC               
HphI          860  TCACC                GGTGA(8/7)
              989  TCACC               
KspI          239  CCGCGG               CCGC^GG
              835  CCGCGG              
NaeI          463  GCCGGC               GCC^GGC
              689  GCCGGC              
NgoMIV        463  GCCGGC               G^CCGGC
              689  GCCGGC              
PleI          404  GACTC                GAGTC(4/5)
              514  GACTC               
SacI          304  GAGCTC               GAGCT^C
              703  GAGCTC              
SacII         239  CCGCGG               CCGC^GG
              835  CCGCGG              
SapI          111  GCTCTTC              GCTCTTC(1/4)
              306  GCTCTTC             
SmaI           20  CCCGGG               CCC^GGG
              210  CCCGGG              
TaqI          560  TCGA                 T^CGA
              572  TCGA                
TthHB8I       560  TCGA                 T^CGA
              572  TCGA                
XmaCI          20  CCCGGG               C^CCGGG
              210  CCCGGG              
XmaI           20  CCCGGG               C^CCGGG
              210  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AvaI        BanI        BanII       BbeI        BbvI        
BcnI        BglI        BmyI        BsaHI       BsaJI       
BseRI       BsiEI       BsiYI       BslI        BsoBI       
Bsp1286I    BsrBI       BsrFI       BsrI        BssHII      
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DsaI        
EaeI        EarI        EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
Hin1I       HinP1I      HincII      HindII      HpaII       
ItaI        KasI        Ksp632I     MaeII       MboII       
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NarI        NciI        NlaIV       RsaI        
Sau96I      ScrFI       SfaNI       SfoI        TseI        
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