Restriction map for pF1KB8805
 (Linear) (Six-base) MAPSORT of: KIBB8805.seq  Check: 6604  from: 1  to: 1053



KIBB8805.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AccI GT'mk_AC

Cuts at:      0     698    1013    1053 
   Size:        698     315      40

  Fragments arranged by size: 

                 698     315      40
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     894    1053 
   Size:        894     159
 
AgeI A'CCGG_T

Cuts at:      0     217    1053 
   Size:        217     836
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     121    1053 
   Size:        121     932
 
AlwNI CAG_nnn'CTG

Cuts at:      0      84    1053 
   Size:         84     969
 
ApaI G_GGCC'C

Cuts at:      0     319     554    1053 
   Size:        319     235     499

  Fragments arranged by size: 

                 499     319     235
 
AvaI C'yCGr_G

Cuts at:      0      20     210     301    1053 
   Size:         20     190      91     752

  Fragments arranged by size: 

                 752     190      91      20
 
BanI G'GyrC_C

Cuts at:      0      25     214     487     530     564     669     686     841 
   Size:         25     189     273      43      34     105      17     155

Cuts at:    841    1053 
   Size:        212

  Fragments arranged by size: 

                 273     212     189     155     105      43      34      25
                  17
 
BanII G_rGCy'C

Cuts at:      0     308     319     554     707    1053 
   Size:        308      11     235     153     346

  Fragments arranged by size: 

                 346     308     235     153      11
 
BbeI G_GCGC'C

Cuts at:      0      29     491     673     690     845    1053 
   Size:         29     462     182      17     155     208

  Fragments arranged by size: 

                 462     208     182     155      29      17
 
BglI GCCn_nnn'nGGC

Cuts at:      0     545     549     684    1053 
   Size:        545       4     135     369

  Fragments arranged by size: 

                 545     369     135       4
 
BlpI GC'TnA_GC

Cuts at:      0     397     706    1053 
   Size:        397     309     347

  Fragments arranged by size: 

                 397     347     309
 
Bme1580I G_kGCm'C

Cuts at:      0     217     319     554     667    1053 
   Size:        217     102     235     113     386

  Fragments arranged by size: 

                 386     235     217     113     102
 
BmgBI CAC'GTC

Cuts at:      0     609    1053 
   Size:        609     444
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     313    1053 
   Size:        313     740
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      27     208    1053 
   Size:         27     181     845

  Fragments arranged by size: 

                 845     181      27
 
BsaHI Gr'CG_yC

Cuts at:      0      26     488     670     687     842    1053 
   Size:         26     462     182      17     155     211

  Fragments arranged by size: 

                 462     211     182     155      26      17
 
BsaWI w'CCGG_w

Cuts at:      0     217    1053 
   Size:        217     836
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     151     175     181     205    1053 
   Size:        151      24       6      24     848

  Fragments arranged by size: 

                 848     151      24      24       6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     156     892    1053 
   Size:        156     736     161

  Fragments arranged by size: 

                 736     161     156
 
BseYI C'CCAG_C

Cuts at:      0     409    1053 
   Size:        409     644
 
BsiEI CG_ry'CG

Cuts at:      0     464     576     827    1053 
   Size:        464     112     251     226

  Fragments arranged by size: 

                 464     251     226     112
 
BsiHKAI G_wGCw'C

Cuts at:      0     308     707    1053 
   Size:        308     399     346

  Fragments arranged by size: 

                 399     346     308
 
Bsp1286I G_dGCh'C

Cuts at:      0     217     308     319     554     667     707    1053 
   Size:        217      91      11     235     113      40     346

  Fragments arranged by size: 

                 346     235     217     113      91      40      11
 
BsrBI CCG'CTC

Cuts at:      0     111     401     570     578    1053 
   Size:        111     290     169       8     475

  Fragments arranged by size: 

                 475     290     169     111       8
 
BsrFI r'CCGG_y

Cuts at:      0     217     362     463     689     727     991    1053 
   Size:        217     145     101     226      38     264      62

  Fragments arranged by size: 

                 264     226     217     145     101      62      38
 
BssHII G'CGCG_C

Cuts at:      0     413     799     879    1053 
   Size:        413     386      80     174

  Fragments arranged by size: 

                 413     386     174      80
 
BssSI C'ACGA_G

Cuts at:      0     276     921    1053 
   Size:        276     645     132

  Fragments arranged by size: 

                 645     276     132
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      84    1053 
   Size:         84     969
 
BtgI C'CryG_G

Cuts at:      0      14     239     835    1053 
   Size:         14     225     596     218

  Fragments arranged by size: 

                 596     225     218      14
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      84     459     477     570     939    1053 
   Size:         84     375      18      93     369     114

  Fragments arranged by size: 

                 375     369     114      93      84      18
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     607    1053 
   Size:        607     446
 
EaeI y'GGCC_r

Cuts at:      0      92     246     461     691     824     891    1053 
   Size:         92     154     215     230     133      67     162

  Fragments arranged by size: 

                 230     215     162     154     133      92      67
 
EagI C'GGCC_G

Cuts at:      0     461     824    1053 
   Size:        461     363     229

  Fragments arranged by size: 

                 461     363     229
 
EarI CTCTTCn'nnn_

Cuts at:      0     118     313     511    1053 
   Size:        118     195     198     542

  Fragments arranged by size: 

                 542     198     195     118
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     796    1053 
   Size:        796     257
 
EcoICRI GAG'CTC

Cuts at:      0     306     705    1053 
   Size:        306     399     348

  Fragments arranged by size: 

                 399     348     306
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      27     208     894    1053 
   Size:         27     181     686     159

  Fragments arranged by size: 

                 686     181     159      27
 
EcoO109I rG'GnC_Cy

Cuts at:      0     315     551    1053 
   Size:        315     236     502

  Fragments arranged by size: 

                 502     315     236
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     960    1053 
   Size:        960      93
 
FseI GG_CCGG'CC

Cuts at:      0     467    1053 
   Size:        467     586
 
FspI TGC'GCA

Cuts at:      0     763    1053 
   Size:        763     290
 
FspAI rTGC'GCAy

Cuts at:      0     763    1053 
   Size:        763     290
 
HaeII r_GCGC'y

Cuts at:      0      14      29      66     262     369     491     540     673 
   Size:         14      15      37     196     107     122      49     133

Cuts at:    673     690     775     845    1053 
   Size:         17      85      70     208

  Fragments arranged by size: 

                 208     196     133     122     107      85      70      49
                  37      17      15      14
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     505     537    1053 
   Size:        505      32     516

  Fragments arranged by size: 

                 516     505      32
 
HincII GTy'rAC

Cuts at:      0     432     636     855    1053 
   Size:        432     204     219     198

  Fragments arranged by size: 

                 432     219     204     198
 
KasI G'GCGC_C

Cuts at:      0      25     487     669     686     841    1053 
   Size:         25     462     182      17     155     212

  Fragments arranged by size: 

                 462     212     182     155      25      17
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     194    1053 
   Size:        194     859
 
MscI TGG'CCA

Cuts at:      0     248    1053 
   Size:        248     805
 
MspA1I CmG'CkG

Cuts at:      0      84     241     396     630     837    1053 
   Size:         84     157     155     234     207     216

  Fragments arranged by size: 

                 234     216     207     157     155      84
 
NaeI GCC'GGC

Cuts at:      0     465     691    1053 
   Size:        465     226     362

  Fragments arranged by size: 

                 465     362     226
 
NarI GG'CG_CC

Cuts at:      0      26     488     670     687     842    1053 
   Size:         26     462     182      17     155     211

  Fragments arranged by size: 

                 462     211     182     155      26      17
 
NgoMIV G'CCGG_C

Cuts at:      0     463     689    1053 
   Size:        463     226     364

  Fragments arranged by size: 

                 463     364     226
 
PspOMI G'GGCC_C

Cuts at:      0     315     550    1053 
   Size:        315     235     503

  Fragments arranged by size: 

                 503     315     235
 
PvuII CAG'CTG

Cuts at:      0     396    1053 
   Size:        396     657
 
SacI G_AGCT'C

Cuts at:      0     308     707    1053 
   Size:        308     399     346

  Fragments arranged by size: 

                 399     346     308
 
SacII CC_GC'GG

Cuts at:      0     242     838    1053 
   Size:        242     596     215

  Fragments arranged by size: 

                 596     242     215
 
SapI GCTCTTCn'nnn_

Cuts at:      0     118     313    1053 
   Size:        118     195     740

  Fragments arranged by size: 

                 740     195     118
 
SexAI A'CCwGG_T

Cuts at:      0     962    1053 
   Size:        962      91
 
SfoI GGC'GCC

Cuts at:      0      27     489     671     688     843    1053 
   Size:         27     462     182      17     155     210

  Fragments arranged by size: 

                 462     210     182     155      27      17
 
SmaI CCC'GGG

Cuts at:      0      22     212    1053 
   Size:         22     190     841

  Fragments arranged by size: 

                 841     190      22
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     613     919    1053 
   Size:        613     306     134

  Fragments arranged by size: 

                 613     306     134
 
XmaI C'CCGG_G

Cuts at:      0      20     210    1053 
   Size:         20     190     843

  Fragments arranged by size: 

                 843     190      20
 
XmnI GAAnn'nnTTC

Cuts at:      0     943    1053 
   Size:        943     110
 

 Enzymes that do cut:

     AccI     AcuI     AgeI     AlfI    AlwNI     ApaI     AvaI     BanI
    BanII     BbeI     BglI     BlpI Bme1580I    BmgBI     BmrI     BpmI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I
    BsrBI    BsrFI   BssHII    BssSI   BstAPI     BtgI    BtgZI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII     KasI     MmeI
     MscI   MspA1I     NaeI     NarI   NgoMIV   PspOMI    PvuII     SacI
    SacII     SapI    SexAI     SfoI     SmaI    TaqII     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AhdI
     AleI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
     BsgI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrDI
    BsrGI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtsI
     ClaI    CspCI     DraI   DraIII    EcoNI    EcoRI    EcoRV  HindIII
     HpaI     KpnI     MfeI     MluI     MslI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PstI     PvuI    RsrII     SalI    SanDI     SbfI     ScaI
     SfcI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     ZraI
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