Restriction map for
pF1KB8798
(Circular) (Six-base) MAPSORT of: pF1KB8798.seq Check: 5394 from: 1 to: 4121
pF1KB8798 4121 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 623 1159 623
Size: 536 3585
Acc65I G'GTAC_C
Cuts at: 1137 1137
Size: 4121
AclI AA'CG_TT
Cuts at: 3961 3961
Size: 4121
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1946 2378 3125 3393 1946
Size: 432 747 268 2674
Fragments arranged by size:
2674 747 432 268
AflIII A'CryG_T
Cuts at: 124 1473 2577 3554 124
Size: 1349 1104 977 691
Fragments arranged by size:
1349 1104 977 691
AgeI A'CCGG_T
Cuts at: 2509 2509
Size: 4121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 325 325
Size: 4121
AlwNI CAG_nnn'CTG
Cuts at: 1426 2993 1426
Size: 1567 2554
ApaLI G'TGCA_C
Cuts at: 2891 2891
Size: 4121
ApoI r'AATT_y
Cuts at: 541 1125 3474 3730 541
Size: 584 2349 256 932
Fragments arranged by size:
2349 932 584 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4121
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4121
AvaI C'yCGr_G
Cuts at: 1141 1141
Size: 4121
BamHI G'GATC_C
Cuts at: 1146 1146
Size: 4121
BanI G'GyrC_C
Cuts at: 1137 1551 1799 1834 1137
Size: 414 248 35 3424
Fragments arranged by size:
3424 414 248 35
BanII G_rGCy'C
Cuts at: 1135 2165 1135
Size: 1030 3091
BbeI G_GCGC'C
Cuts at: 1555 1803 1555
Size: 248 3873
BbsI GAAGACnn'nnnn_
Cuts at: 1066 1066
Size: 4121
BciVI GTATCCnnnnn_n'
Cuts at: 2019 2780 4121 2019
Size: 761 1341 2019
Fragments arranged by size:
2019 1341 761
BclI T'GATC_A
Cuts at: 1641 1641
Size: 4121
BfrBI ATG'CAT
Cuts at: 405 575 405
Size: 170 3951
BglII A'GATC_T
Cuts at: 1636 3673 1636
Size: 2037 2084
BlpI GC'TnA_GC
Cuts at: 1233 1233
Size: 4121
Bme1580I G_kGCm'C
Cuts at: 1746 1839 2895 1746
Size: 93 1056 2972
Fragments arranged by size:
2972 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 607 1333 1414 1749 3893 607
Size: 726 81 335 2144 835
Fragments arranged by size:
2144 835 726 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2488 2488
Size: 4121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1297 2176 2668 2966 1297
Size: 879 492 298 2452
Fragments arranged by size:
2452 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 721 3825 721
Size: 3104 1017
BsaAI yAC'GTr
Cuts at: 1474 1474
Size: 4121
BsaBI GATnn'nnATC
Cuts at: 6 1635 3672 6
Size: 1629 2037 455
Fragments arranged by size:
2037 1629 455
BsaHI Gr'CG_yC
Cuts at: 1552 1800 2502 3998 1552
Size: 248 702 1496 1675
Fragments arranged by size:
1675 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1310 1831 2509 2783 2930 3341 1310
Size: 521 678 274 147 411 2090
Fragments arranged by size:
2090 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1546 2304 2881 3377 1546
Size: 758 577 496 2290
Fragments arranged by size:
2290 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 321 321
Size: 4121
BsiEI CG_ry'CG
Cuts at: 104 1663 1709 2917 104
Size: 1559 46 1208 1308
Fragments arranged by size:
1559 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1135 1913 2103 2895 1135
Size: 778 190 792 2361
Fragments arranged by size:
2361 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1135 1746 1839 1913 2103 2165 2895 1135
Size: 611 93 74 190 62 730 2361
Fragments arranged by size:
2361 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3341 3341
Size: 4121
BspHI T'CATG_A
Cuts at: 317 3297 317
Size: 2980 1141
BspMI ACCTGCnnnn'nnnn_
Cuts at: 703 1171 1385 1687 2068 703
Size: 468 214 302 381 2756
Fragments arranged by size:
2756 468 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2413 2467 3951 4116 50
Size: 2363 54 1484 165 55
Fragments arranged by size:
2363 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2033 2033
Size: 4121
BsrFI r'CCGG_y
Cuts at: 2119 2300 2509 2119
Size: 181 209 3731
Fragments arranged by size:
3731 209 181
BssSI C'ACGA_G
Cuts at: 2392 2750 2392
Size: 358 3763
BstAPI GCAn_nnn'nTGC
Cuts at: 1498 1498
Size: 4121
BstBI TT'CG_AA
Cuts at: 2482 2482
Size: 4121
BstYI r'GATC_y
Cuts at: 1 1146 1613 1636 1971 2217 3229 3315 3327
Size: 1145 467 23 335 246 1012 86 12
Cuts at: 3327 3673 1
Size: 346 449
Fragments arranged by size:
1145 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 624 624
Size: 4121
BtgI C'CryG_G
Cuts at: 152 152
Size: 4121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2071 2252 2415 2071
Size: 181 163 3777
Fragments arranged by size:
3777 181 163
BtsI GCAGTG_nn'
Cuts at: 309 1364 309
Size: 1055 3066
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 717 752 717
Size: 35 4086
DraI TTT'AAA
Cuts at: 912 1120 912
Size: 208 3913
DraIII CAC_nnn'GTG
Cuts at: 279 279
Size: 4121
DrdI GACnn_nn'nnGTC
Cuts at: 1827 2685 1827
Size: 858 3263
EaeI y'GGCC_r
Cuts at: 525 1004 1706 1880 2271 2298 3643 525
Size: 479 702 174 391 27 1345 1003
Fragments arranged by size:
1345 1003 702 479 391 174 27
EagI C'GGCC_G
Cuts at: 1706 1706
Size: 4121
EarI CTCTTCn'nnn_
Cuts at: 153 650 2144 2354 153
Size: 497 1494 210 1920
Fragments arranged by size:
1920 1494 497 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2329 2639 2785 3931 2329
Size: 310 146 1146 2519
Fragments arranged by size:
2519 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1133 1133
Size: 4121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1946 2378 3125 3393 1946
Size: 432 747 268 2674
Fragments arranged by size:
2674 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1260 1260
Size: 4121
EcoRI G'AATT_C
Cuts at: 1125 1125
Size: 4121
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2140 2140
Size: 4121
FspI TGC'GCA
Cuts at: 1902 1902
Size: 4121
HaeII r_GCGC'y
Cuts at: 1555 1803 2825 3804 1555
Size: 248 1022 979 1872
Fragments arranged by size:
1872 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 683 699 715 731 1963 1993 1995 2025 683
Size: 16 16 16 1232 30 2 30 2779
Fragments arranged by size:
2779 1232 30 30 16 16 16 2
HincII GTy'rAC
Cuts at: 1160 1160
Size: 4121
KasI G'GCGC_C
Cuts at: 1551 1799 1551
Size: 248 3873
KpnI G_GTAC'C
Cuts at: 1141 1141
Size: 4121
MfeI C'AATT_G
Cuts at: 955 955
Size: 4121
MluI A'CGCG_T
Cuts at: 3554 3554
Size: 4121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 421 2792 2976 421
Size: 2371 184 1566
Fragments arranged by size:
2371 1566 184
MscI TGG'CCA
Cuts at: 527 1882 527
Size: 1355 2766
MslI CAynn'nnrTG
Cuts at: 2110 2237 2110
Size: 127 3994
MspA1I CmG'CkG
Cuts at: 1232 1546 1906 2919 3164 1232
Size: 314 360 1013 245 2189
Fragments arranged by size:
2189 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2302 2302
Size: 4121
NarI GG'CG_CC
Cuts at: 1552 1800 1552
Size: 248 3873
NcoI C'CATG_G
Cuts at: 152 152
Size: 4121
NdeI CA'TA_TG
Cuts at: 583 1100 1110 583
Size: 517 10 3594
Fragments arranged by size:
3594 517 10
NgoMIV G'CCGG_C
Cuts at: 2300 2300
Size: 4121
NsiI A_TGCA'T
Cuts at: 407 577 407
Size: 170 3951
NspI r_CATG'y
Cuts at: 128 409 1174 1674 2581 128
Size: 281 765 500 907 1668
Fragments arranged by size:
1668 907 765 500 281
PciI A'CATG_T
Cuts at: 124 2577 124
Size: 2453 1668
PmeI GTTT'AAAC
Cuts at: 1120 1120
Size: 4121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3260 3292 3260
Size: 32 4089
PstI C_TGCA'G
Cuts at: 1168 1853 1168
Size: 685 3436
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4121
PvuII CAG'CTG
Cuts at: 1546 1906 1546
Size: 360 3761
RsrII CG'GwC_CG
Cuts at: 2316 2316
Size: 4121
SacI G_AGCT'C
Cuts at: 1135 1135
Size: 4121
SalI G'TCGA_C
Cuts at: 1158 1158
Size: 4121
SapI GCTCTTCn'nnn_
Cuts at: 2144 2354 2144
Size: 210 3911
SbfI CC_TGCA'GG
Cuts at: 1168 1168
Size: 4121
ScaI AGT'ACT
Cuts at: 758 3638 758
Size: 2880 1241
SfcI C'TryA_G
Cuts at: 33 644 1164 1849 2842 3033 33
Size: 611 520 685 993 191 1121
Fragments arranged by size:
1121 993 685 611 520 191
SfoI GGC'GCC
Cuts at: 1553 1801 1553
Size: 248 3873
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4121
SmaI CCC'GGG
Cuts at: 1143 1143
Size: 4121
SmlI C'TyrA_G
Cuts at: 1276 2191 2683 2945 1276
Size: 915 492 262 2452
Fragments arranged by size:
2452 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4121
SphI G_CATG'C
Cuts at: 409 1174 1674 409
Size: 765 500 2856
Fragments arranged by size:
2856 765 500
SspI AAT'ATT
Cuts at: 518 518
Size: 4121
StyI C'CwwG_G
Cuts at: 152 1255 1609 152
Size: 1103 354 2664
Fragments arranged by size:
2664 1103 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1704 1837 2296 2505 1704
Size: 133 459 209 3320
Fragments arranged by size:
3320 459 209 133
TatI w'GTAC_w
Cuts at: 594 756 3636 594
Size: 162 2880 1079
Fragments arranged by size:
2880 1079 162
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3638 3670 3638
Size: 32 4089
Tth111I GACn'n_nGTC
Cuts at: 1918 1918
Size: 4121
XbaI T'CTAG_A
Cuts at: 1152 1152
Size: 4121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1616 1616
Size: 4121
XmaI C'CCGG_G
Cuts at: 1141 1141
Size: 4121
XmnI GAAnn'nnTTC
Cuts at: 340 340
Size: 4121
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI Bsu36I ClaI EcoNI EcoRV FseI
FspAI HindIII HpaI NheI NotI NruI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI