Restriction map for pF1KB8791
 (Linear) (Six-base) MAPSORT of: KIBB8791.seq  Check: 5949  from: 1  to: 957



KIBB8791.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     117     957 
   Size:        117     840
 
AflII C'TTAA_G

Cuts at:      0     145     957 
   Size:        145     812
 
AflIII A'CryG_T

Cuts at:      0     497     512     957 
   Size:        497      15     445

  Fragments arranged by size: 

                 497     445      15
 
AgeI A'CCGG_T

Cuts at:      0     353     957 
   Size:        353     604
 
AleI CACnn'nnGTG

Cuts at:      0     286     957 
   Size:        286     671
 
AlwNI CAG_nnn'CTG

Cuts at:      0     750     957 
   Size:        750     207
 
ApaI G_GGCC'C

Cuts at:      0     903     957 
   Size:        903      54
 
ApaLI G'TGCA_C

Cuts at:      0     279     957 
   Size:        279     678
 
ApoI r'AATT_y

Cuts at:      0     880     957 
   Size:        880      77
 
AvaI C'yCGr_G

Cuts at:      0     950     957 
   Size:        950       7
 
BanII G_rGCy'C

Cuts at:      0     903     957 
   Size:        903      54
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     318     534     693     957 
   Size:        318     216     159     264

  Fragments arranged by size: 

                 318     264     216     159
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     151     185     957 
   Size:        151      34     772

  Fragments arranged by size: 

                 772     151      34
 
BclI T'GATC_A

Cuts at:      0     188     957 
   Size:        188     769
 
BglI GCCn_nnn'nGGC

Cuts at:      0     694     957 
   Size:        694     263
 
BglII A'GATC_T

Cuts at:      0     410     957 
   Size:        410     547
 
Bme1580I G_kGCm'C

Cuts at:      0     283     903     957 
   Size:        283     620      54

  Fragments arranged by size: 

                 620     283      54
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     660     715     957 
   Size:        660      55     242

  Fragments arranged by size: 

                 660     242      55
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     549     574     588     957 
   Size:        549      25      14     369

  Fragments arranged by size: 

                 549     369      25      14
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     629     714     957 
   Size:        629      85     243

  Fragments arranged by size: 

                 629     243      85
 
BsaWI w'CCGG_w

Cuts at:      0     353     395     451     957 
   Size:        353      42      56     506

  Fragments arranged by size: 

                 506     353      56      42
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      97     957 
   Size:         97     860
 
BseYI C'CCAG_C

Cuts at:      0     624     957 
   Size:        624     333
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     411     676     798     957 
   Size:        411     265     122     159

  Fragments arranged by size: 

                 411     265     159     122
 
BsiHKAI G_wGCw'C

Cuts at:      0     224     283     957 
   Size:        224      59     674

  Fragments arranged by size: 

                 674     224      59
 
Bsp1286I G_dGCh'C

Cuts at:      0     224     283     903     957 
   Size:        224      59     620      54

  Fragments arranged by size: 

                 620     224      59      54
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     122     218     957 
   Size:        122      96     739

  Fragments arranged by size: 

                 739     122      96
 
BsrBI CCG'CTC

Cuts at:      0     378     957 
   Size:        378     579
 
BsrFI r'CCGG_y

Cuts at:      0     353     905     957 
   Size:        353     552      52

  Fragments arranged by size: 

                 552     353      52
 
BstYI r'GATC_y

Cuts at:      0     410     419     957 
   Size:        410       9     538

  Fragments arranged by size: 

                 538     410       9
 
BtgI C'CryG_G

Cuts at:      0     126     477     578     957 
   Size:        126     351     101     379

  Fragments arranged by size: 

                 379     351     126     101
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     666     957 
   Size:        666     291
 
EaeI y'GGCC_r

Cuts at:      0     119     362     957 
   Size:        119     243     595

  Fragments arranged by size: 

                 595     243     119
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     117     549     574     588     957 
   Size:        117     432      25      14     369

  Fragments arranged by size: 

                 432     369     117      25      14
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     250     957 
   Size:        250     707
 
EcoO109I rG'GnC_Cy

Cuts at:      0     438     899     957 
   Size:        438     461      58

  Fragments arranged by size: 

                 461     438      58
 
EcoRI G'AATT_C

Cuts at:      0     880     957 
   Size:        880      77
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     561     957 
   Size:        561     396
 
HaeII r_GCGC'y

Cuts at:      0     536     957 
   Size:        536     421
 
HincII GTy'rAC

Cuts at:      0      45     837     957 
   Size:         45     792     120

  Fragments arranged by size: 

                 792     120      45
 
MfeI C'AATT_G

Cuts at:      0     811     957 
   Size:        811     146
 
MscI TGG'CCA

Cuts at:      0     121     364     957 
   Size:        121     243     593

  Fragments arranged by size: 

                 593     243     121
 
MslI CAynn'nnrTG

Cuts at:      0     184     286     346     957 
   Size:        184     102      60     611

  Fragments arranged by size: 

                 611     184     102      60
 
MspA1I CmG'CkG

Cuts at:      0     552     924     957 
   Size:        552     372      33

  Fragments arranged by size: 

                 552     372      33
 
NcoI C'CATG_G

Cuts at:      0     126     477     578     957 
   Size:        126     351     101     379

  Fragments arranged by size: 

                 379     351     126     101
 
NspI r_CATG'y

Cuts at:      0     315     501     516     957 
   Size:        315     186      15     441

  Fragments arranged by size: 

                 441     315     186      15
 
PciI A'CATG_T

Cuts at:      0     497     512     957 
   Size:        497      15     445

  Fragments arranged by size: 

                 497     445      15
 
PfoI T'CCnGG_A

Cuts at:      0     604     957 
   Size:        604     353
 
PpuMI rG'GwC_Cy

Cuts at:      0     438     957 
   Size:        438     519
 
PspOMI G'GGCC_C

Cuts at:      0     899     957 
   Size:        899      58
 
PstI C_TGCA'G

Cuts at:      0     746     935     957 
   Size:        746     189      22

  Fragments arranged by size: 

                 746     189      22
 
PvuII CAG'CTG

Cuts at:      0     924     957 
   Size:        924      33
 
SbfI CC_TGCA'GG

Cuts at:      0     935     957 
   Size:        935      22
 
SfcI C'TryA_G

Cuts at:      0      64     468     675     742     931     957 
   Size:         64     404     207      67     189      26

  Fragments arranged by size: 

                 404     207     189      67      64      26
 
SmaI CCC'GGG

Cuts at:      0     952     957 
   Size:        952       5
 
SmlI C'TyrA_G

Cuts at:      0     145     957 
   Size:        145     812
 
StyI C'CwwG_G

Cuts at:      0     126     477     536     578     957 
   Size:        126     351      59      42     379

  Fragments arranged by size: 

                 379     351     126      59      42
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     642     658     957 
   Size:        642      16     299

  Fragments arranged by size: 

                 642     299      16
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     382     414     895     927     957 
   Size:        382      32     481      32      30

  Fragments arranged by size: 

                 481     382      32      32      30
 
Tth111I GACn'n_nGTC

Cuts at:      0      40     957 
   Size:         40     917
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      93     438     585     921     957 
   Size:         93     345     147     336      36

  Fragments arranged by size: 

                 345     336     147      93      36
 
XmaI C'CCGG_G

Cuts at:      0     950     957 
   Size:        950       7
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AgeI     AleI    AlwNI     ApaI    ApaLI
     ApoI     AvaI    BanII     BbsI     BcgI     BclI     BglI    BglII
 Bme1580I     BmrI     BpmI     BsaI    BsaWI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrFI    BstYI     BtgI    BtgZI
     EaeI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII   HincII
     MfeI     MscI     MslI   MspA1I     NcoI     NspI     PciI     PfoI
    PpuMI   PspOMI     PstI    PvuII     SbfI     SfcI     SmaI     SmlI
     StyI    TaqII     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AhdI     AlfI
     AloI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI    BciVI    BfrBI     BlpI    BmgBI     BmtI     BplI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
    EcoRV     FseI     FspI    FspAI    Hin4I  HindIII     HpaI     KasI
     KpnI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI     XbaI     XhoI     XmnI     ZraI
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