Restriction map for pF1KB8786
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       AccI        AccII       AclI        AcsI        
AcyI        AflII       AgeI        AhdI        AocI        
Aor51HI     ApoI        AscI        AseI        AsnI        
AspEI       AspI        AviII       AvrII       BamHI       
BbeI        BbvCI       BcgI        BciVI       BcnI        
BfaI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaBI       BsaHI       BsaI        BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspHI       
BsrBI       BsrFI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HaeII       Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KspI        
MaeI        MamI        MfeI        MluI        MseI        
MunI        MvnI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tru9I       
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

Acc65I        410  GGTACC               G^GTACC
AccIII        833  TCCGGA               T^CCGGA
Alw44I        580  GTGCAC               G^TGCAC
AlwI          602  GATCC                GGATC(4/5)
AlwNI         882  CAGGTACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          695  GGGCCC               GGGCC^C
ApaLI         580  GTGCAC               G^TGCAC
Asp700I        47  GAAATAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       410  GGTACC               G^GTACC
AvaI          673  CTCGGG               C^[CT]CG[GA]G
AvaII         378  GGACC                G^G[AT]CC
BalI          197  TGGCCA               TGG^CCA
BbrPI         281  CACGTG               CAC^GTG
BpmI          163  CTCCAG               CTGGAG(16/14)
BsaAI         281  CACGTG               [CT]AC^GT[GA]
BsaJI         491  CCATGG               C^C[ACGT][ACGT]GG
BsaWI         833  TCCGGA               [AT]^CCGG[AT]
BseAI         833  TCCGGA               T^CCGGA
BseRI          82  GAGGAG               GAGGAG(10/8)
BsgI          471  CTGCAC               GTGCAG(16/14)
BsmAI         765  GTCTC                GTCTC(1/5)
BsmI          856  GCATTC               GAATGC(1/-1)
BsoBI         673  CTCGGG               C^[CT]CG[GA]G
BspEI         833  TCCGGA               T^CCGGA
BspMI         881  GCAGGT               ACCTGC(4/8)
BsrDI         800  GCAATG               GCAATG(2/0)
BssSI          65  CTCGTG               CACGAG(-5/-1)
CpoI          377  CGGACCG              CG^G[AT]CCG
DdeI          847  CTCAG                C^T[ACGT]AG
DraII         695  GGGCCCC              [GA]G^G[ACGT]CC[CT]
DsaI          491  CCATGG               C^C[GA][CT]GG
EaeI          197  TGGCCA               [CT]^GGCC[GA]
EarI          769  CTCTTC               CTCTTC(1/4)
EcoO109I      695  GGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoT14I       491  CCATGG               C^C[AT][AT]GG
FauI          546  CCCGC                CCCGC(4/6)
HgaI          853  GACGC                GACGC(5/10)
KpnI          410  GGTACC               GGTAC^C
Ksp632I       769  CTCTTC               CTCTTC(1/4)
MluNI         197  TGGCCA               TGG^CCA
MroI          833  TCCGGA               T^CCGGA
MscI          197  TGGCCA               TGG^CCA
MspA1I        547  CCGCTG               C[AC]G^C[GT]G
MwoI          540  GCTGTTCCCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          491  CCATGG               C^CATGG
PleI          319  GACTC                GAGTC(4/5)
PmaCI         281  CACGTG               CAC^GTG
PmlI          281  CACGTG               CAC^GTG
PstI          876  CTGCAG               CTGCA^G
RsrII         377  CGGACCG              CG^G[AT]CCG
SacI          844  GAGCTC               GAGCT^C
SmlI          274  CTCAAG               C^T[CT][GA]AG
StyI          491  CCATGG               C^C[AT][AT]GG
Tsp45I        424  GTGAC                ^GT[GC]AC
XmnI           47  GAAATAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AflIII        584  ACATGT               A^C[GA][CT]GT
              761  ACATGT              
AluI          193  AGCT                 AG^CT
              845  AGCT                
AspHI         580  GTGCAC               G[AT]GC[AT]^C
              844  GAGCTC              
BanI          168  GGCACC               G^G[CT][GA]CC
              410  GGTACC              
BbsI          559  GTCTTC               GAAGAC(2/6)
              679  GTCTTC              
BglII         230  AGATCT               A^GATCT
              617  AGATCT              
BpuAI         559  GTCTTC               GAAGAC(2/6)
              679  GTCTTC              
BsiHKAI       580  GTGCAC               G[AT]GC[AT]^C
              844  GAGCTC              
BsmFI         478  GGGAC                GGGAC(10/14)
              851  GGGAC               
BspLU11I      584  ACATGT               A^CATGT
              761  ACATGT              
BsrI          481  ACTGG                ACTGG(1/-1)
              887  ACTGG               
BstF5I        564  CATCC                GGATG(2/0)
              666  CATCC               
BstYI         230  AGATCT               [GA]^GATC[CT]
              617  AGATCT              
CfoI          432  GCGC                 GCG^C
              752  GCGC                
FokI          564  CATCC                GGATG(9/13)
              666  CATCC               
HapII          79  CCGG                 C^CGG
              834  CCGG                
HhaI          432  GCGC                 GCG^C
              752  GCGC                
HinP1I        432  GCGC                 G^CGC
              752  GCGC                
HpaII          79  CCGG                 C^CGG
              834  CCGG                
MaeIII        424  GTGAC                ^GT[ACGT]AC
              740  GTAAC               
MflI          230  AGATCT               [GA]^GATC[CT]
              617  AGATCT              
MspI           79  CCGG                 C^CGG
              834  CCGG                
SfcI          189  CTGTAG               C^T[GA][CT]AG
              876  CTGCAG              
TaqI          317  TCGA                 T^CGA
              600  TCGA                
TfiI          123  GAATC                G^A[AT]TC
              159  GAATC               
Tsp509I        52  AATT                 ^AATT
               99  AATT                
TthHB8I       317  TCGA                 T^CGA
              600  TCGA                
XhoII         230  AGATCT               [GA]^GATC[CT]
              617  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AciI        AfaI        BanII       BbvI        BclI        
BmyI        BsiYI       BslI        Bsp1286I    BssKI       
BstNI       Cac8I       Cfr13I      DpnII       EcoRII      
FbaI        Fnu4HI      HaeIII      HinfI       HphI        
ItaI        MaeII       MboI        MboII       MnlI        
MslI        MvaI        NdeII       NlaIII      NlaIV       
NspI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       TseI        TspRI       
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