Restriction map for pF1KB8786
 (Linear) (Six-base) MAPSORT of: KIBB8786.seq  Check: 6484  from: 1  to: 891



KIBB8786.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

Acc65I G'GTAC_C

Cuts at:      0     410     891 
   Size:        410     481
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     339     891 
   Size:        339     552
 
AflIII A'CryG_T

Cuts at:      0     584     761     891 
   Size:        584     177     130

  Fragments arranged by size: 

                 584     177     130
 
AleI CACnn'nnGTG

Cuts at:      0     442     493     891 
   Size:        442      51     398

  Fragments arranged by size: 

                 442     398      51
 
AlwNI CAG_nnn'CTG

Cuts at:      0     887     891 
   Size:        887       4
 
ApaI G_GGCC'C

Cuts at:      0     699     891 
   Size:        699     192
 
ApaLI G'TGCA_C

Cuts at:      0     580     891 
   Size:        580     311
 
AvaI C'yCGr_G

Cuts at:      0     673     891 
   Size:        673     218
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     394     427     891 
   Size:        394      33     464

  Fragments arranged by size: 

                 464     394      33
 
BanI G'GyrC_C

Cuts at:      0     168     410     891 
   Size:        168     242     481

  Fragments arranged by size: 

                 481     242     168
 
BanII G_rGCy'C

Cuts at:      0     699     841     848     891 
   Size:        699     142       7      43

  Fragments arranged by size: 

                 699     142      43       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     552     672     891 
   Size:        552     120     219

  Fragments arranged by size: 

                 552     219     120
 
BclI T'GATC_A

Cuts at:      0     248     524     791     891 
   Size:        248     276     267     100

  Fragments arranged by size: 

                 276     267     248     100
 
BglII A'GATC_T

Cuts at:      0     230     617     891 
   Size:        230     387     274

  Fragments arranged by size: 

                 387     274     230
 
Bme1580I G_kGCm'C

Cuts at:      0     518     584     699     891 
   Size:        518      66     115     192

  Fragments arranged by size: 

                 518     192     115      66
 
BmgBI CAC'GTC

Cuts at:      0     536     891 
   Size:        536     355
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     891 
   Size:        148     743
 
Bpu10I CC'TnA_GC

Cuts at:      0     847     891 
   Size:        847      44
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259     891 
   Size:        259     632
 
BsaAI yAC'GTr

Cuts at:      0     283     891 
   Size:        283     608
 
BsaWI w'CCGG_w

Cuts at:      0     833     891 
   Size:        833      58
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      97     891 
   Size:         97     794
 
BseYI C'CCAG_C

Cuts at:      0     213     692     891 
   Size:        213     479     199

  Fragments arranged by size: 

                 479     213     199
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     456     891 
   Size:        456     435
 
BsiHKAI G_wGCw'C

Cuts at:      0     584     848     891 
   Size:        584     264      43

  Fragments arranged by size: 

                 584     264      43
 
BsmI GAATG_Cn'

Cuts at:      0     856     891 
   Size:        856      35
 
Bsp1286I G_dGCh'C

Cuts at:      0     518     584     699     841     848     891 
   Size:        518      66     115     142       7      43

  Fragments arranged by size: 

                 518     142     115      66      43       7
 
BspEI T'CCGG_A

Cuts at:      0     833     891 
   Size:        833      58
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     872     891 
   Size:        872      19
 
BsrDI GCAATG_nn'

Cuts at:      0     807     891 
   Size:        807      84
 
BssSI C'ACGA_G

Cuts at:      0      65     891 
   Size:         65     826
 
BstYI r'GATC_y

Cuts at:      0     230     617     891 
   Size:        230     387     274

  Fragments arranged by size: 

                 387     274     230
 
BtgI C'CryG_G

Cuts at:      0     491     891 
   Size:        491     400
 
BtsI GCAGTG_nn'

Cuts at:      0     403     891 
   Size:        403     488
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     264     299     891 
   Size:        264      35     592

  Fragments arranged by size: 

                 592     264      35
 
EaeI y'GGCC_r

Cuts at:      0     197     891 
   Size:        197     694
 
EarI CTCTTCn'nnn_

Cuts at:      0     775     891 
   Size:        775     116
 
EcoICRI GAG'CTC

Cuts at:      0     846     891 
   Size:        846      45
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     339     891 
   Size:        148     191     552

  Fragments arranged by size: 

                 552     191     148
 
EcoO109I rG'GnC_Cy

Cuts at:      0     696     891 
   Size:        696     195
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     888     891 
   Size:        888       3
 
KpnI G_GTAC'C

Cuts at:      0     414     891 
   Size:        414     477
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      80      90     891 
   Size:         80      10     801

  Fragments arranged by size: 

                 801      80      10
 
MscI TGG'CCA

Cuts at:      0     199     891 
   Size:        199     692
 
MslI CAynn'nnrTG

Cuts at:      0     442     490     493     891 
   Size:        442      48       3     398

  Fragments arranged by size: 

                 442     398      48       3
 
MspA1I CmG'CkG

Cuts at:      0     549     891 
   Size:        549     342
 
NcoI C'CATG_G

Cuts at:      0     491     891 
   Size:        491     400
 
NspI r_CATG'y

Cuts at:      0     588     765     789     891 
   Size:        588     177      24     102

  Fragments arranged by size: 

                 588     177     102      24
 
PciI A'CATG_T

Cuts at:      0     584     761     891 
   Size:        584     177     130

  Fragments arranged by size: 

                 584     177     130
 
PmlI CAC'GTG

Cuts at:      0     283     891 
   Size:        283     608
 
PspOMI G'GGCC_C

Cuts at:      0     695     891 
   Size:        695     196
 
PstI C_TGCA'G

Cuts at:      0     880     891 
   Size:        880      11
 
RsrII CG'GwC_CG

Cuts at:      0     378     891 
   Size:        378     513
 
SacI G_AGCT'C

Cuts at:      0     848     891 
   Size:        848      43
 
SfcI C'TryA_G

Cuts at:      0     189     876     891 
   Size:        189     687      15

  Fragments arranged by size: 

                 687     189      15
 
SmlI C'TyrA_G

Cuts at:      0     274     891 
   Size:        274     617
 
StyI C'CwwG_G

Cuts at:      0     491     891 
   Size:        491     400
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     186     891 
   Size:        186     705
 
TatI w'GTAC_w

Cuts at:      0     176     891 
   Size:        176     715
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     650     682     891 
   Size:        650      32     209

  Fragments arranged by size: 

                 650     209      32
 
XmnI GAAnn'nnTTC

Cuts at:      0      51     891 
   Size:         51     840
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AleI    AlwNI     ApaI    ApaLI     AvaI
     BaeI     BanI    BanII     BbsI     BclI    BglII Bme1580I    BmgBI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaWI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI    BsrDI    BssSI    BstYI
     BtgI     BtsI    CspCI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I
     FalI     KpnI     MmeI     MscI     MslI   MspA1I     NcoI     NspI
     PciI     PmlI   PspOMI     PstI    RsrII     SacI     SfcI     SmlI
     StyI    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI     ApoI     AscI     AseI    AsiSI    AvrII    BamHI
     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI     BmrI
     BmtI     BplI     BsaI    BsaBI    BsaHI    BsaXI    BsiEI    BsiWI
    BsmBI    BspHI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI     DraI   DraIII
     DrdI     EagI     EciI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     MfeI
     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com