Restriction map for pF1KB8780
##################################
# NO CUT ENZYMES (  155 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BglII       BlnI        BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstYI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HaeII       
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeII       MamI        
MfeI        MflI        MluI        MroI        MseI        
MslI        MunI        NarI        NcoI        NdeI        
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StyI        
SwaI        TfiI        Tru9I       Tsp509I     Tth111I     
Van91I      XbaI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccII         189  CGCG                 CG^CG
AspHI         235  GTGCTC               G[AT]GC[AT]^C
AvaI          333  CCCGGG               C^[CT]CG[GA]G
BbsI          772  GTCTTC               GAAGAC(2/6)
BfaI          229  CTAG                 C^TAG
BglI          577  GCCACCGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          405  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      405  GCTCAGC              GC^T[ACGT]AGC
BpuAI         772  GTCTTC               GAAGAC(2/6)
BseRI         649  GAGGAG               GAGGAG(10/8)
BsiHKAI       235  GTGCTC               G[AT]GC[AT]^C
BsoBI         333  CCCGGG               C^[CT]CG[GA]G
BsrBI         415  CCGCTC               CCGCTC(-3/-3)
BsrFI         659  GCCGGC               [GA]^CCGG[CT]
BstUI         189  CGCG                 CG^CG
BstXI         604  CCAGGCTACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         405  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        659  GCCGGC               [GA]^CCGG[CT]
DpnII          45  GATC                 ^GATC
EarI          762  CTCTTC               CTCTTC(1/4)
HgaI          549  GCGTC                GACGC(5/10)
Ksp632I       762  CTCTTC               CTCTTC(1/4)
MaeI          229  CTAG                 C^TAG
MaeIII         66  GTCAC                ^GT[ACGT]AC
MboI           45  GATC                 ^GATC
MvnI          189  CGCG                 CG^CG
NaeI          659  GCCGGC               GCC^GGC
NdeII          45  GATC                 ^GATC
NgoMIV        659  GCCGGC               G^CCGGC
NheI          228  GCTAGC               G^CTAGC
PpuMI         692  GGGACCT              [GA]G^G[AT]CC[CT]
Sau3AI         45  GATC                 ^GATC
SmaI          333  CCCGGG               CCC^GGG
StuI          653  AGGCCT               AGG^CCT
TaqI          479  TCGA                 T^CGA
Tsp45I         66  GTCAC                ^GT[GC]AC
TthHB8I       479  TCGA                 T^CGA
XcmI          710  CCAGCGACAGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         333  CCCGGG               C^CCGGG
XmaI          333  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AfaI          375  GTAC                 GT^AC
              739  GTAC                
AlwNI          55  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              346  CAGTGTCTG           
ApaI          204  GGGCCC               GGGCC^C
              753  GGGCCC              
BalI          278  TGGCCA               TGG^CCA
              326  TGGCCA              
BanII         204  GGGCCC               G[GA]GC[CT]^C
              753  GGGCCC              
BsaI          389  GAGACC               GGTCTC(1/5)
              530  GAGACC              
BspMI         377  ACCTGC               ACCTGC(4/8)
              735  GCAGGT              
BstF5I         96  CATCC                GGATG(2/0)
              642  CATCC               
CfoI          190  GCGC                 GCG^C
              222  GCGC                
DdeI          406  CTCAG                C^T[ACGT]AG
              742  CTCAG               
EaeI          278  TGGCCA               [CT]^GGCC[GA]
              326  TGGCCA              
EcoNI         645  CCTGGAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              720  CCTGGCCCAGG         
FokI           96  CATCC                GGATG(9/13)
              642  CATCC               
HhaI          190  GCGC                 GCG^C
              222  GCGC                
HinP1I        190  GCGC                 G^CGC
              222  GCGC                
HphI          484  GGTGA                GGTGA(8/7)
              194  TCACC               
MboII         763  TCTTC                GAAGA(8/7)
              773  TCTTC               
MluNI         278  TGGCCA               TGG^CCA
              326  TGGCCA              
MscI          278  TGGCCA               TGG^CCA
              326  TGGCCA              
RsaI          375  GTAC                 GT^AC
              739  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AciI        AluI        AvaII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstNI       Cac8I       Cfr13I      DraII       EcoO109I    
EcoRII      FauI        Fnu4HI      HaeIII      HapII       
HinfI       HpaII       ItaI        MnlI        MspA1I      
MspI        MvaI        MwoI        NciI        NlaIV       
PleI        PstI        PvuII       Sau96I      ScrFI       
SfcI        TseI        TspRI       
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