Restriction map for pF1KB8780
 (Linear) (Six-base) MAPSORT of: KIBB8780.seq  Check: 9606  from: 1  to: 777



KIBB8780.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     127     777 
   Size:         82      45     650

  Fragments arranged by size: 

                 650      82      45
 
AlwNI CAG_nnn'CTG

Cuts at:      0      60     351     777 
   Size:         60     291     426

  Fragments arranged by size: 

                 426     291      60
 
ApaI G_GGCC'C

Cuts at:      0     208     757     777 
   Size:        208     549      20

  Fragments arranged by size: 

                 549     208      20
 
AvaI C'yCGr_G

Cuts at:      0     333     777 
   Size:        333     444
 
BanII G_rGCy'C

Cuts at:      0     208     757     777 
   Size:        208     549      20

  Fragments arranged by size: 

                 549     208      20
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     765     777 
   Size:        765      12
 
BglI GCCn_nnn'nGGC

Cuts at:      0     583     777 
   Size:        583     194
 
BlpI GC'TnA_GC

Cuts at:      0     406     777 
   Size:        406     371
 
Bme1580I G_kGCm'C

Cuts at:      0     208     757     777 
   Size:        208     549      20

  Fragments arranged by size: 

                 549     208      20
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     447     621     777 
   Size:        447     174     156

  Fragments arranged by size: 

                 447     174     156
 
BmtI G_CTAG'C

Cuts at:      0     232     777 
   Size:        232     545
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     184     289     391     543     667     777 
   Size:        109      75     105     102     152     124     110

  Fragments arranged by size: 

                 152     124     110     109     105     102      75
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     383     524     777 
   Size:        383     141     253

  Fragments arranged by size: 

                 383     253     141
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     664     777 
   Size:        664     113
 
BseYI C'CCAG_C

Cuts at:      0      53     355     553     587     625     689     777 
   Size:         53     302     198      34      38      64      88

  Fragments arranged by size: 

                 302     198      88      64      53      38      34
 
BsiHKAI G_wGCw'C

Cuts at:      0     239     777 
   Size:        239     538
 
Bsp1286I G_dGCh'C

Cuts at:      0     208     239     757     777 
   Size:        208      31     518      20

  Fragments arranged by size: 

                 518     208      31      20
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     386     726     777 
   Size:        386     340      51

  Fragments arranged by size: 

                 386     340      51
 
BsrBI CCG'CTC

Cuts at:      0     417     777 
   Size:        417     360
 
BsrFI r'CCGG_y

Cuts at:      0     659     777 
   Size:        659     118
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     611     777 
   Size:        611     166
 
BtsI GCAGTG_nn'

Cuts at:      0     352     676     777 
   Size:        352     324     101

  Fragments arranged by size: 

                 352     324     101
 
EaeI y'GGCC_r

Cuts at:      0     278     326     777 
   Size:        278      48     451

  Fragments arranged by size: 

                 451     278      48
 
EarI CTCTTCn'nnn_

Cuts at:      0     768     777 
   Size:        768       9
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      50     745     777 
   Size:         50     695      32

  Fragments arranged by size: 

                 695      50      32
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     109     127     184     289     391     543     667 
   Size:         82      27      18      57     105     102     152     124

Cuts at:    667     777 
   Size:        110

  Fragments arranged by size: 

                 152     124     110     105     102      82      57      27
                  18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     649     724     777 
   Size:        649      75      53

  Fragments arranged by size: 

                 649      75      53
 
EcoO109I rG'GnC_Cy

Cuts at:      0     568     693     754     777 
   Size:        568     125      61      23

  Fragments arranged by size: 

                 568     125      61      23
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      29      61     777 
   Size:         29      32     716

  Fragments arranged by size: 

                 716      32      29
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     272     777 
   Size:        272     505
 
MscI TGG'CCA

Cuts at:      0     280     328     777 
   Size:        280      48     449

  Fragments arranged by size: 

                 449     280      48
 
MspA1I CmG'CkG

Cuts at:      0      13      57     210     342     522     777 
   Size:         13      44     153     132     180     255

  Fragments arranged by size: 

                 255     180     153     132      44      13
 
NaeI GCC'GGC

Cuts at:      0     661     777 
   Size:        661     116
 
NgoMIV G'CCGG_C

Cuts at:      0     659     777 
   Size:        659     118
 
NheI G'CTAG_C

Cuts at:      0     228     777 
   Size:        228     549
 
PasI CC'CwG_GG

Cuts at:      0     593     777 
   Size:        593     184
 
PfoI T'CCnGG_A

Cuts at:      0     449     644     668     777 
   Size:        449     195      24     109

  Fragments arranged by size: 

                 449     195     109      24
 
PpuMI rG'GwC_Cy

Cuts at:      0     693     777 
   Size:        693      84
 
PspOMI G'GGCC_C

Cuts at:      0     204     753     777 
   Size:        204     549      24

  Fragments arranged by size: 

                 549     204      24
 
PstI C_TGCA'G

Cuts at:      0      44     347     447     683     777 
   Size:         44     303     100     236      94

  Fragments arranged by size: 

                 303     236     100      94      44
 
PvuII CAG'CTG

Cuts at:      0      57     342     522     777 
   Size:         57     285     180     255

  Fragments arranged by size: 

                 285     255     180      57
 
SfcI C'TryA_G

Cuts at:      0      40     343     443     679     777 
   Size:         40     303     100     236      98

  Fragments arranged by size: 

                 303     236     100      98      40
 
SmaI CCC'GGG

Cuts at:      0     335     777 
   Size:        335     442
 
StuI AGG'CCT

Cuts at:      0     655     777 
   Size:        655     122
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     211     585     777 
   Size:        211     374     192

  Fragments arranged by size: 

                 374     211     192
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     717     777 
   Size:        717      60
 
XmaI C'CCGG_G

Cuts at:      0     333     777 
   Size:        333     444
 

 Enzymes that do cut:

     AcuI    AlwNI     ApaI     AvaI    BanII     BbsI     BglI     BlpI
 Bme1580I     BmrI     BmtI     BpmI     BsaI    BseRI    BseYI  BsiHKAI
 Bsp1286I    BspMI    BsrBI    BsrFI    BstXI     BtsI     EaeI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    Hin4I     MmeI     MscI   MspA1I
     NaeI   NgoMIV     NheI     PasI     PfoI    PpuMI   PspOMI     PstI
    PvuII     SfcI     SmaI     StuI    TaqII     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI     BplI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MslI     NarI
     NcoI     NdeI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StyI     SwaI     TatI     TstI
  Tth111I     XbaI     XhoI     XmnI     ZraI
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