Restriction map for pF1KB8772
##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BanI        BanII       BbeI        BcgI        BciVI       
BcnI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaBI       BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HpaII       KasI        KpnI        KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmeI        Psp1406I    PstI        PvuI        
RsrII       SacI        SacII       SalI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SwaI        Tth111I     Van91I      XbaI        
XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   40 enzymes) #
###################################

AflII         955  CTTAAG               C^TTAAG
AlwNI         113  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI         1659  ATTAAT               AT^TAAT
AsnI         1659  ATTAAT               AT^TAAT
AspHI        1621  GAGCAC               G[AT]GC[AT]^C
BamHI         969  GGATCC               G^GATCC
BbrPI         905  CACGTG               CAC^GTG
BbsI         1143  GTCTTC               GAAGAC(2/6)
BbvI         1227  GCAGC                GCAGC(8/12)
BfrI          955  CTTAAG               C^TTAAG
BpuAI        1143  GTCTTC               GAAGAC(2/6)
BsaAI         905  CACGTG               [CT]AC^GT[GA]
BseRI         770  CTCCTC               GAGGAG(10/8)
BsgI         1225  GTGCAG               GTGCAG(16/14)
BsiHKAI      1621  GAGCAC               G[AT]GC[AT]^C
BsmFI          13  GGGAC                GGGAC(10/14)
BsrDI         172  GCAATG               GCAATG(2/0)
BstAPI        717  GCAATCCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI         1356  TTTAAA               TTT^AAA
DraIII       1279  CACTATGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I      1538  CCAAGG               C^C[AT][AT]GG
Fnu4HI       1227  GCAGC                GC^[ACGT]GC
HpaI          960  GTTAAC               GTT^AAC
ItaI         1227  GCAGC                GC^[ACGT]GC
MaeII         906  ACGT                 A^CGT
MspA1I       1228  CAGCTG               C[AC]G^C[GT]G
MwoI          717  GCAATCCTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PmaCI         905  CACGTG               CAC^GTG
PmlI          905  CACGTG               CAC^GTG
PshAI         255  GACTAGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1659  ATTAAT               AT^TAAT
PvuII        1228  CAGCTG               CAG^CTG
SapI          532  GAAGAGC              GCTCTTC(1/4)
SfaNI        1007  GCATC                GCATC(5/9)
SfcI          198  CTACAG               C^T[GA][CT]AG
StuI          887  AGGCCT               AGG^CCT
StyI         1538  CCAAGG               C^C[AT][AT]GG
TaqI          783  TCGA                 T^CGA
TseI         1227  GCAGC                G^C[AT]GC
TthHB8I       783  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcsI         1267  AAATTT               [GA]^AATT[CT]
             1503  AAATTT              
AfaI          350  GTAC                 GT^AC
             1420  GTAC                
ApoI         1267  AAATTT               [GA]^AATT[CT]
             1503  AAATTT              
BbvCI          40  GCTGAGG              CCTCAGC(-5/-2)
              118  GCTGAGG             
BclI          230  TGATCA               T^GATCA
             1406  TGATCA              
BmyI         1252  GGGCAC               G[AGT]GC[ACT]^C
             1621  GAGCAC              
Bsp1286I     1252  GGGCAC               G[AGT]GC[ACT]^C
             1621  GAGCAC              
BspHI         981  TCATGA               T^CATGA
             1044  TCATGA              
DraII         403  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              547  GGGTCCT             
EcoNI         538  CCTAGTGTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              727  CCTCCAGAAGG         
EcoO109I      403  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              547  GGGTCCT             
FbaI          230  TGATCA               T^GATCA
             1406  TGATCA              
HincII          4  GTTGAC               GT[CT]^[GA]AC
              960  GTTAAC              
HindII          4  GTTGAC               GT[CT]^[GA]AC
              960  GTTAAC              
HphI          163  GGTGA                GGTGA(8/7)
             1313  TCACC               
MslI          330  CATGCCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              471  CATTGGGATG          
PpuMI         403  AGGTCCT              [GA]G^G[AT]CC[CT]
              547  GGGTCCT             
RcaI          981  TCATGA               T^CATGA
             1044  TCATGA              
RsaI          350  GTAC                 GT^AC
             1420  GTAC                
ScaI          349  AGTACT               AGT^ACT
             1419  AGTACT              
XcmI          150  CCAGGCCAAAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              730  CCAGAAGGAAACTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AluI        AlwI        AvaII       BfaI        BpmI        
BsaI        BsaJI       BsiYI       BslI        BsmAI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       EarI        
EcoRII      FokI        HaeIII      HinfI       Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MvaI        NdeII       NlaIII      
NlaIV       PleI        Sau3AI      Sau96I      ScrFI       
SmlI        SspI        TfiI        Tru9I       Tsp45I      
Tsp509I     TspRI       XhoII       
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