Restriction map for pF1KB8772
 (Linear) (Six-base) MAPSORT of: KIBB8772.seq  Check: 5481  from: 1  to: 1674



KIBB8772.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     386     584    1674 
   Size:        386     198    1090

  Fragments arranged by size: 

                1090     386     198
 
AflII C'TTAA_G

Cuts at:      0     955    1674 
   Size:        955     719
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     322    1674 
   Size:        322    1352
 
AlwNI CAG_nnn'CTG

Cuts at:      0     118    1674 
   Size:        118    1556
 
ApoI r'AATT_y

Cuts at:      0    1267    1503    1674 
   Size:       1267     236     171

  Fragments arranged by size: 

                1267     236     171
 
AseI AT'TA_AT

Cuts at:      0    1660    1674 
   Size:       1660      14
 
BamHI G'GATC_C

Cuts at:      0     969    1674 
   Size:        969     705
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1136    1674 
   Size:       1136     538
 
BbvCI CC'TCA_GC

Cuts at:      0      41     119    1674 
   Size:         41      78    1555

  Fragments arranged by size: 

                1555      78      41
 
BclI T'GATC_A

Cuts at:      0     230    1406    1674 
   Size:        230    1176     268

  Fragments arranged by size: 

                1176     268     230
 
Bme1580I G_kGCm'C

Cuts at:      0    1256    1674 
   Size:       1256     418
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     446    1674 
   Size:        446    1228
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     548     713    1130    1674 
   Size:        548     165     417     544

  Fragments arranged by size: 

                 548     544     417     165
 
Bpu10I CC'TnA_GC

Cuts at:      0      41     119    1674 
   Size:         41      78    1555

  Fragments arranged by size: 

                1555      78      41
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1016    1278    1674 
   Size:       1016     262     396

  Fragments arranged by size: 

                1016     396     262
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     457     565     748    1674 
   Size:        457     108     183     926

  Fragments arranged by size: 

                 926     457     183     108
 
BsaAI yAC'GTr

Cuts at:      0     907    1674 
   Size:        907     767
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     761    1674 
   Size:        761     913
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1246    1674 
   Size:       1246     428
 
BsiHKAI G_wGCw'C

Cuts at:      0    1625    1674 
   Size:       1625      49
 
Bsp1286I G_dGCh'C

Cuts at:      0    1256    1625    1674 
   Size:       1256     369      49

  Fragments arranged by size: 

                1256     369      49
 
BspHI T'CATG_A

Cuts at:      0     981    1044    1674 
   Size:        981      63     630

  Fragments arranged by size: 

                 981     630      63
 
BsrDI GCAATG_nn'

Cuts at:      0     179    1674 
   Size:        179    1495
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     723    1674 
   Size:        723     951
 
BstYI r'GATC_y

Cuts at:      0     605     797     969    1674 
   Size:        605     192     172     705

  Fragments arranged by size: 

                 705     605     192     172
 
BtsI GCAGTG_nn'

Cuts at:      0      77     119     894    1674 
   Size:         77      42     775     780

  Fragments arranged by size: 

                 780     775      77      42
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     888     923    1674 
   Size:        888      35     751

  Fragments arranged by size: 

                 888     751      35
 
DraI TTT'AAA

Cuts at:      0    1358    1674 
   Size:       1358     316
 
DraIII CAC_nnn'GTG

Cuts at:      0    1284    1674 
   Size:       1284     390
 
EarI CTCTTCn'nnn_

Cuts at:      0     455     527     563     584    1674 
   Size:        455      72      36      21    1090

  Fragments arranged by size: 

                1090     455      72      36      21
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     386     548     584     713    1130    1674 
   Size:        386     162      36     129     417     544

  Fragments arranged by size: 

                 544     417     386     162     129      36
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     542     731    1674 
   Size:        542     189     943

  Fragments arranged by size: 

                 943     542     189
 
EcoO109I rG'GnC_Cy

Cuts at:      0     404     548    1674 
   Size:        404     144    1126

  Fragments arranged by size: 

                1126     404     144
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     753     785    1120    1152    1391    1423    1634    1666 
   Size:        753      32     335      32     239      32     211      32

Cuts at:   1666    1674 
   Size:          8

  Fragments arranged by size: 

                 753     335     239     211      32      32      32      32
                   8
 
HincII GTy'rAC

Cuts at:      0       6     962    1674 
   Size:          6     956     712

  Fragments arranged by size: 

                 956     712       6
 
HpaI GTT'AAC

Cuts at:      0     962    1674 
   Size:        962     712
 
MslI CAynn'nnrTG

Cuts at:      0     334     475    1674 
   Size:        334     141    1199

  Fragments arranged by size: 

                1199     334     141
 
MspA1I CmG'CkG

Cuts at:      0    1230    1674 
   Size:       1230     444
 
PfoI T'CCnGG_A

Cuts at:      0     608    1577    1674 
   Size:        608     969      97

  Fragments arranged by size: 

                 969     608      97
 
PmlI CAC'GTG

Cuts at:      0     907    1674 
   Size:        907     767
 
PpuMI rG'GwC_Cy

Cuts at:      0     404     548    1674 
   Size:        404     144    1126

  Fragments arranged by size: 

                1126     404     144
 
PshAI GACnn'nnGTC

Cuts at:      0     259    1674 
   Size:        259    1415
 
PsiI TTA'TAA

Cuts at:      0     590    1674 
   Size:        590    1084
 
PvuII CAG'CTG

Cuts at:      0    1230    1674 
   Size:       1230     444
 
SapI GCTCTTCn'nnn_

Cuts at:      0     527    1674 
   Size:        527    1147
 
ScaI AGT'ACT

Cuts at:      0     351    1421    1674 
   Size:        351    1070     253

  Fragments arranged by size: 

                1070     351     253
 
SfcI C'TryA_G

Cuts at:      0     198    1674 
   Size:        198    1476
 
SmlI C'TyrA_G

Cuts at:      0     955    1031    1257    1674 
   Size:        955      76     226     417

  Fragments arranged by size: 

                 955     417     226      76
 
SspI AAT'ATT

Cuts at:      0     399    1470    1479    1490    1574    1674 
   Size:        399    1071       9      11      84     100

  Fragments arranged by size: 

                1071     399     100      84      11       9
 
StuI AGG'CCT

Cuts at:      0     889    1674 
   Size:        889     785
 
StyI C'CwwG_G

Cuts at:      0    1538    1674 
   Size:       1538     136
 
TatI w'GTAC_w

Cuts at:      0     349    1419    1674 
   Size:        349    1070     255

  Fragments arranged by size: 

                1070     349     255
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     157     737    1674 
   Size:        157     580     937

  Fragments arranged by size: 

                 937     580     157
 

 Enzymes that do cut:

     AcuI    AflII     AlfI    AlwNI     ApoI     AseI    BamHI     BbsI
    BbvCI     BclI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BseRI     BsgI  BsiHKAI Bsp1286I    BspHI    BsrDI   BstAPI
    BstYI     BtsI    CspCI     DraI   DraIII     EarI  Eco57MI    EcoNI
 EcoO109I    Hin4I   HincII     HpaI     MslI   MspA1I     PfoI     PmlI
    PpuMI    PshAI     PsiI    PvuII     SapI     ScaI     SfcI     SmlI
     SspI     StuI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI     BanI    BanII     BbeI     BcgI    BciVI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII  HindIII     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PpiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SwaI    TaqII
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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