Restriction map for pF1KB8768
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# NO CUT ENZYMES (  168 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaII       
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmFI       BsmI        Bsp1407I    BspDI       
BspEI       BspLU11I    BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstPI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraII       
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FokI        FseI        FspI        Hin1I       HincII      
HindII      HindIII     HinfI       HpaI        KasI        
KpnI        KspI        MaeI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
TaqI        TfiI        Tru9I       Tth111I     TthHB8I     
XbaI        XhoI        XhoII       


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# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI          343  GTCTAC               GT^[AC][GT]AC
Asp700I       381  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII         622  GAGCCC               G[GA]GC[CT]^C
BmyI          622  GAGCCC               G[AGT]GC[ACT]^C
BpmI          616  CTCCAG               CTGGAG(16/14)
Bsp1286I      622  GAGCCC               G[AGT]GC[ACT]^C
BspHI         189  TCATGA               T^CATGA
BsrBI         174  GAGCGG               CCGCTC(-3/-3)
BsrFI         610  ACCGGC               [GA]^CCGG[CT]
BsrI          225  CCAGT                ACTGG(1/-1)
BstXI         437  CCATCGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        610  ACCGGC               [GA]^CCGG[CT]
DraIII        244  CACGGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          243  CCACGG               C^C[GA][CT]GG
EarI          373  GAAGAG               CTCTTC(1/4)
EcoT14I        23  CCAAGG               C^C[AT][AT]GG
FauI           61  CCCGC                CCCGC(4/6)
HaeII         305  AGCGCC               [GA]GCGC^[CT]
HgaI          116  GCGTC                GACGC(5/10)
Ksp632I       373  GAAGAG               CTCTTC(1/4)
MaeII         311  ACGT                 A^CGT
MslI          678  CATAATCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        134  CCGCTG               C[AC]G^C[GT]G
NspI          578  ACATGC               [GA]CATG^[CT]
PflMI         225  CCAGTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI          189  TCATGA               T^CATGA
SapI          373  GAAGAGC              GCTCTTC(1/4)
SfaNI         642  GATGC                GCATC(5/9)
SmaI          320  CCCGGG               CCC^GGG
StuI          383  AGGCCT               AGG^CCT
StyI           23  CCAAGG               C^C[AT][AT]GG
Tsp45I        352  GTCAC                ^GT[GC]AC
TspRI         654  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I        225  CCAGTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         320  CCCGGG               C^CCGGG
XmaI          320  CCCGGG               C^CCGGG
XmnI          381  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AfaI          152  GTAC                 GT^AC
              195  GTAC                
AvaI          320  CCCGGG               C^[CT]CG[GA]G
              625  CCCGAG              
BanI          130  GGCACC               G^G[CT][GA]CC
              496  GGTGCC              
BsoBI         320  CCCGGG               C^[CT]CG[GA]G
              625  CCCGAG              
BspMI         197  ACCTGC               ACCTGC(4/8)
              422  ACCTGC              
Cfr13I        324  GGGCC                G^G[ACGT]CC
              483  GGCCC               
DdeI          348  CTCAG                C^T[ACGT]AG
              656  CTGAG               
HphI          266  TCACC                GGTGA(8/7)
              353  TCACC               
PstI          237  CTGCAG               CTGCA^G
              301  CTGCAG              
RsaI          152  GTAC                 GT^AC
              195  GTAC                
Sau96I        324  GGGCC                G^G[ACGT]CC
              483  GGCCC               
SfcI          237  CTGCAG               C^T[GA][CT]AG
              301  CTGCAG              
Tsp509I       520  AATT                 ^AATT
              589  AATT                
XcmI          437  CCATCGTGCTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              500  CCACCTGTGCGATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AccII       AciI        AluI        AlwNI       BbvI        
BcnI        BsaJI       BsgI        BsiYI       BslI        
BssKI       BstNI       BstUI       Cac8I       CfoI        
DpnII       EcoRII      Fnu4HI      HaeIII      HapII       
HhaI        HinP1I      HpaII       ItaI        MaeIII      
MboI        MboII       MnlI        MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       Sau3AI      ScrFI       TseI        
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