Restriction map for
pF1KB8767
(Circular) (Six-base) MAPSORT of: pF1KB8767.seq Check: 6791 from: 1 to: 3788
pF1KB8767 3788 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 826 826
Size: 3788
Acc65I G'GTAC_C
Cuts at: 804 804
Size: 3788
AclI AA'CG_TT
Cuts at: 3628 3628
Size: 3788
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 658 1613 2045 2792 3060 165
Size: 493 955 432 747 268 893
Fragments arranged by size:
955 893 747 493 432 268
AflIII A'CryG_T
Cuts at: 1140 2244 3221 1140
Size: 1104 977 1707
Fragments arranged by size:
1707 1104 977
AgeI A'CCGG_T
Cuts at: 2176 2176
Size: 3788
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 578 578
Size: 3788
AlwNI CAG_nnn'CTG
Cuts at: 1093 2660 1093
Size: 1567 2221
ApaI G_GGCC'C
Cuts at: 746 746
Size: 3788
ApaLI G'TGCA_C
Cuts at: 478 2558 478
Size: 2080 1708
ApoI r'AATT_y
Cuts at: 792 3141 3397 792
Size: 2349 256 1183
Fragments arranged by size:
2349 1183 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3788
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
AvaI C'yCGr_G
Cuts at: 550 808 550
Size: 258 3530
BamHI G'GATC_C
Cuts at: 813 813
Size: 3788
BanI G'GyrC_C
Cuts at: 304 546 804 1218 1466 1501 304
Size: 242 258 414 248 35 2591
Fragments arranged by size:
2591 414 258 248 242 35
BanII G_rGCy'C
Cuts at: 299 507 746 802 1832 299
Size: 208 239 56 1030 2255
Fragments arranged by size:
2255 1030 239 208 56
BbeI G_GCGC'C
Cuts at: 1222 1470 1222
Size: 248 3540
BbsI GAAGACnn'nnnn_
Cuts at: 697 697
Size: 3788
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 264 298 667 701 264
Size: 34 369 34 3351
Fragments arranged by size:
3351 369 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1686 2447 3788 1686
Size: 761 1341 1686
Fragments arranged by size:
1686 1341 761
BclI T'GATC_A
Cuts at: 1308 1308
Size: 3788
BglII A'GATC_T
Cuts at: 1303 3340 1303
Size: 2037 1751
BlpI GC'TnA_GC
Cuts at: 352 900 352
Size: 548 3240
Bme1580I G_kGCm'C
Cuts at: 452 482 551 746 1413 1506 2562 452
Size: 30 69 195 667 93 1056 1678
Fragments arranged by size:
1678 1056 667 195 93 69 30
BmrI ACTGGGnnnn_n'
Cuts at: 360 1000 1081 1416 3560 360
Size: 640 81 335 2144 588
Fragments arranged by size:
2144 640 588 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2155 2155
Size: 3788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 270 521 270
Size: 251 3537
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 454 964 1843 2335 2633 157
Size: 297 510 879 492 298 1312
Fragments arranged by size:
1312 879 510 492 298 297
BsaI GGTCTCn'nnnn_
Cuts at: 3492 3492
Size: 3788
BsaAI yAC'GTr
Cuts at: 1141 1141
Size: 3788
BsaBI GATnn'nnATC
Cuts at: 6 1302 3339 6
Size: 1296 2037 455
Fragments arranged by size:
2037 1296 455
BsaHI Gr'CG_yC
Cuts at: 1219 1467 2169 3665 1219
Size: 248 702 1496 1342
Fragments arranged by size:
1496 1342 702 248
BsaWI w'CCGG_w
Cuts at: 977 1498 2176 2450 2597 3008 977
Size: 521 678 274 147 411 1757
Fragments arranged by size:
1757 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 595 625 697 727 595
Size: 30 72 30 3656
Fragments arranged by size:
3656 72 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 528 645 528
Size: 117 3671
BseYI C'CCAG_C
Cuts at: 566 731 1213 1971 2548 3044 566
Size: 165 482 758 577 496 1310
Fragments arranged by size:
1310 758 577 496 482 165
BsiEI CG_ry'CG
Cuts at: 104 1330 1376 2584 104
Size: 1226 46 1208 1308
Fragments arranged by size:
1308 1226 1208 46
BsiHKAI G_wGCw'C
Cuts at: 439 470 482 802 1580 1770 2562 439
Size: 31 12 320 778 190 792 1665
Fragments arranged by size:
1665 792 778 320 190 31 12
Bsp1286I G_dGCh'C
Cuts at: 299 439 452 470 482 507 551 746 802
Size: 140 13 18 12 25 44 195 56
Cuts at: 802 1413 1506 1580 1770 1832 2562 299
Size: 611 93 74 190 62 730 1525
Fragments arranged by size:
1525 730 611 195 190 140 93 74
62 56 44 25 18 13 12
BspEI T'CCGG_A
Cuts at: 3008 3008
Size: 3788
BspHI T'CATG_A
Cuts at: 2964 2964
Size: 3788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 287 392 838 1052 1354 1735 287
Size: 105 446 214 302 381 2340
Fragments arranged by size:
2340 446 381 302 214 105
BsrBI CCG'CTC
Cuts at: 50 239 2080 2134 3618 3783 50
Size: 189 1841 54 1484 165 55
Fragments arranged by size:
1841 1484 189 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1700 1700
Size: 3788
BsrFI r'CCGG_y
Cuts at: 262 301 644 1786 1967 2176 262
Size: 39 343 1142 181 209 1874
Fragments arranged by size:
1874 1142 343 209 181 39
BssSI C'ACGA_G
Cuts at: 2059 2417 2059
Size: 358 3430
BstAPI GCAn_nnn'nTGC
Cuts at: 447 1165 447
Size: 718 3070
BstBI TT'CG_AA
Cuts at: 2149 2149
Size: 3788
BstEII G'GTnAC_C
Cuts at: 111 111
Size: 3788
BstYI r'GATC_y
Cuts at: 1 626 813 1280 1303 1638 1884 2896 2982
Size: 625 187 467 23 335 246 1012 86
Cuts at: 2982 2994 3340 1
Size: 12 346 449
Fragments arranged by size:
1012 625 467 449 346 335 246 187
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3788
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1738 1919 2082 1738
Size: 181 163 3444
Fragments arranged by size:
3444 181 163
BtsI GCAGTG_nn'
Cuts at: 224 263 1031 224
Size: 39 768 2981
Fragments arranged by size:
2981 768 39
DraI TTT'AAA
Cuts at: 787 787
Size: 3788
DrdI GACnn_nn'nnGTC
Cuts at: 1494 2352 1494
Size: 858 2930
EaeI y'GGCC_r
Cuts at: 665 702 1373 1547 1938 1965 3310 665
Size: 37 671 174 391 27 1345 1143
Fragments arranged by size:
1345 1143 671 391 174 37 27
EagI C'GGCC_G
Cuts at: 1373 1373
Size: 3788
EarI CTCTTCn'nnn_
Cuts at: 588 677 1811 2021 588
Size: 89 1134 210 2355
Fragments arranged by size:
2355 1134 210 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1996 2306 2452 3598 1996
Size: 310 146 1146 2186
Fragments arranged by size:
2186 1146 310 146
EcoICRI GAG'CTC
Cuts at: 800 800
Size: 3788
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 270 521 658 1613 2045 2792 3060 165
Size: 105 251 137 955 432 747 268 893
Fragments arranged by size:
955 893 747 432 268 251 137 105
EcoO109I rG'GnC_Cy
Cuts at: 742 927 742
Size: 185 3603
EcoRI G'AATT_C
Cuts at: 792 792
Size: 3788
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1807 1807
Size: 3788
FspI TGC'GCA
Cuts at: 223 388 1569 223
Size: 165 1181 2442
Fragments arranged by size:
2442 1181 165
HaeII r_GCGC'y
Cuts at: 339 1222 1470 2492 3471 339
Size: 883 248 1022 979 656
Fragments arranged by size:
1022 979 883 656 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 544 576 1630 1660 1662 1692 544
Size: 32 1054 30 2 30 2640
Fragments arranged by size:
2640 1054 32 30 30 2
HincII GTy'rAC
Cuts at: 699 827 699
Size: 128 3660
KasI G'GCGC_C
Cuts at: 1218 1466 1218
Size: 248 3540
KpnI G_GTAC'C
Cuts at: 808 808
Size: 3788
MluI A'CGCG_T
Cuts at: 3221 3221
Size: 3788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 667 683 2459 2643 667
Size: 16 1776 184 1812
Fragments arranged by size:
1812 1776 184 16
MscI TGG'CCA
Cuts at: 667 1549 667
Size: 882 2906
MslI CAynn'nnrTG
Cuts at: 1777 1904 1777
Size: 127 3661
MspA1I CmG'CkG
Cuts at: 204 735 899 1213 1573 2586 2831 204
Size: 531 164 314 360 1013 245 1161
Fragments arranged by size:
1161 1013 531 360 314 245 164
NaeI GCC'GGC
Cuts at: 303 646 1969 303
Size: 343 1323 2122
Fragments arranged by size:
2122 1323 343
NarI GG'CG_CC
Cuts at: 1219 1467 1219
Size: 248 3540
NcoI C'CATG_G
Cuts at: 107 107
Size: 3788
NgoMIV G'CCGG_C
Cuts at: 301 644 1967 301
Size: 343 1323 2122
Fragments arranged by size:
2122 1323 343
NspI r_CATG'y
Cuts at: 492 841 1341 2248 492
Size: 349 500 907 2032
Fragments arranged by size:
2032 907 500 349
PasI CC'CwG_GG
Cuts at: 577 577
Size: 3788
PciI A'CATG_T
Cuts at: 2244 2244
Size: 3788
PflMI CCAn_nnn'nTGG
Cuts at: 207 577 207
Size: 370 3418
PfoI T'CCnGG_A
Cuts at: 758 758
Size: 3788
PmeI GTTT'AAAC
Cuts at: 787 787
Size: 3788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2927 2959 2927
Size: 32 3756
PspOMI G'GGCC_C
Cuts at: 742 742
Size: 3788
PstI C_TGCA'G
Cuts at: 233 422 835 1520 233
Size: 189 413 685 2501
Fragments arranged by size:
2501 685 413 189
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3788
PvuII CAG'CTG
Cuts at: 204 735 1213 1573 204
Size: 531 478 360 2419
Fragments arranged by size:
2419 531 478 360
RsrII CG'GwC_CG
Cuts at: 1983 1983
Size: 3788
SacI G_AGCT'C
Cuts at: 802 802
Size: 3788
SalI G'TCGA_C
Cuts at: 825 825
Size: 3788
SapI GCTCTTCn'nnn_
Cuts at: 588 1811 2021 588
Size: 1223 210 2355
Fragments arranged by size:
2355 1223 210
SbfI CC_TGCA'GG
Cuts at: 835 835
Size: 3788
ScaI AGT'ACT
Cuts at: 3305 3305
Size: 3788
SfcI C'TryA_G
Cuts at: 33 229 418 831 1516 2509 2700 33
Size: 196 189 413 685 993 191 1121
Fragments arranged by size:
1121 993 685 413 196 191 189
SfoI GGC'GCC
Cuts at: 1220 1468 1220
Size: 248 3540
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
SmaI CCC'GGG
Cuts at: 810 810
Size: 3788
SmlI C'TyrA_G
Cuts at: 172 469 943 1858 2350 2612 172
Size: 297 474 915 492 262 1348
Fragments arranged by size:
1348 915 492 474 297 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3788
SphI G_CATG'C
Cuts at: 841 1341 841
Size: 500 3288
StyI C'CwwG_G
Cuts at: 107 922 1276 107
Size: 815 354 2619
Fragments arranged by size:
2619 815 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 130 745 1371 1504 1963 2172 130
Size: 615 626 133 459 209 1746
Fragments arranged by size:
1746 626 615 459 209 133
TatI w'GTAC_w
Cuts at: 3303 3303
Size: 3788
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 697 729 3305 3337 697
Size: 32 2576 32 1148
Fragments arranged by size:
2576 1148 32 32
Tth111I GACn'n_nGTC
Cuts at: 366 1585 366
Size: 1219 2569
XbaI T'CTAG_A
Cuts at: 819 819
Size: 3788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 574 1283 574
Size: 709 3079
XmaI C'CCGG_G
Cuts at: 808 808
Size: 3788
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI