Restriction map for pF1KB8767
 (Linear) (Six-base) MAPSORT of: KIBB8767.seq  Check: 3073  from: 1  to: 675



KIBB8767.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     550     675 
   Size:         57     493     125

  Fragments arranged by size: 

                 493     125      57
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     470     675 
   Size:        470     205
 
ApaI G_GGCC'C

Cuts at:      0     638     675 
   Size:        638      37
 
ApaLI G'TGCA_C

Cuts at:      0     370     675 
   Size:        370     305
 
AvaI C'yCGr_G

Cuts at:      0     442     675 
   Size:        442     233
 
BanI G'GyrC_C

Cuts at:      0     196     438     675 
   Size:        196     242     237

  Fragments arranged by size: 

                 242     237     196
 
BanII G_rGCy'C

Cuts at:      0     191     399     638     675 
   Size:        191     208     239      37

  Fragments arranged by size: 

                 239     208     191      37
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     589     675 
   Size:        589      86
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     156     190     559     593     675 
   Size:        156      34     369      34      82

  Fragments arranged by size: 

                 369     156      82      34      34
 
BlpI GC'TnA_GC

Cuts at:      0     244     675 
   Size:        244     431
 
Bme1580I G_kGCm'C

Cuts at:      0     344     374     443     638     675 
   Size:        344      30      69     195      37

  Fragments arranged by size: 

                 344     195      69      37      30
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     252     675 
   Size:        252     423
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     162     413     675 
   Size:        162     251     262

  Fragments arranged by size: 

                 262     251     162
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     346     675 
   Size:         49     297     329

  Fragments arranged by size: 

                 329     297      49
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     487     517     589     619     675 
   Size:        487      30      72      30      56

  Fragments arranged by size: 

                 487      72      56      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     420     537     675 
   Size:        420     117     138

  Fragments arranged by size: 

                 420     138     117
 
BseYI C'CCAG_C

Cuts at:      0     458     623     675 
   Size:        458     165      52

  Fragments arranged by size: 

                 458     165      52
 
BsiHKAI G_wGCw'C

Cuts at:      0     331     362     374     675 
   Size:        331      31      12     301

  Fragments arranged by size: 

                 331     301      31      12
 
Bsp1286I G_dGCh'C

Cuts at:      0     191     331     344     362     374     399     443     638 
   Size:        191     140      13      18      12      25      44     195

Cuts at:    638     675 
   Size:         37

  Fragments arranged by size: 

                 195     191     140      44      37      25      18      13
                  12
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     179     284     675 
   Size:        179     105     391

  Fragments arranged by size: 

                 391     179     105
 
BsrBI CCG'CTC

Cuts at:      0     131     675 
   Size:        131     544
 
BsrFI r'CCGG_y

Cuts at:      0     154     193     536     675 
   Size:        154      39     343     139

  Fragments arranged by size: 

                 343     154     139      39
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     339     675 
   Size:        339     336
 
BstEII G'GTnAC_C

Cuts at:      0       3     675 
   Size:          3     672
 
BstYI r'GATC_y

Cuts at:      0     518     675 
   Size:        518     157
 
BtsI GCAGTG_nn'

Cuts at:      0     116     155     675 
   Size:        116      39     520

  Fragments arranged by size: 

                 520     116      39
 
EaeI y'GGCC_r

Cuts at:      0     557     594     675 
   Size:        557      37      81

  Fragments arranged by size: 

                 557      81      37
 
EarI CTCTTCn'nnn_

Cuts at:      0     480     569     675 
   Size:        480      89     106

  Fragments arranged by size: 

                 480     106      89
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     162     413     550     675 
   Size:         57     105     251     137     125

  Fragments arranged by size: 

                 251     137     125     105      57
 
EcoO109I rG'GnC_Cy

Cuts at:      0     634     675 
   Size:        634      41
 
FspI TGC'GCA

Cuts at:      0     115     280     675 
   Size:        115     165     395

  Fragments arranged by size: 

                 395     165     115
 
HaeII r_GCGC'y

Cuts at:      0     231     675 
   Size:        231     444
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     436     468     675 
   Size:        436      32     207

  Fragments arranged by size: 

                 436     207      32
 
HincII GTy'rAC

Cuts at:      0     591     675 
   Size:        591      84
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     559     575     675 
   Size:        559      16     100

  Fragments arranged by size: 

                 559     100      16
 
MscI TGG'CCA

Cuts at:      0     559     675 
   Size:        559     116
 
MspA1I CmG'CkG

Cuts at:      0      96     627     675 
   Size:         96     531      48

  Fragments arranged by size: 

                 531      96      48
 
NaeI GCC'GGC

Cuts at:      0     195     538     675 
   Size:        195     343     137

  Fragments arranged by size: 

                 343     195     137
 
NgoMIV G'CCGG_C

Cuts at:      0     193     536     675 
   Size:        193     343     139

  Fragments arranged by size: 

                 343     193     139
 
NspI r_CATG'y

Cuts at:      0     384     675 
   Size:        384     291
 
PasI CC'CwG_GG

Cuts at:      0     469     675 
   Size:        469     206
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      99     469     675 
   Size:         99     370     206

  Fragments arranged by size: 

                 370     206      99
 
PfoI T'CCnGG_A

Cuts at:      0     650     675 
   Size:        650      25
 
PspOMI G'GGCC_C

Cuts at:      0     634     675 
   Size:        634      41
 
PstI C_TGCA'G

Cuts at:      0     125     314     675 
   Size:        125     189     361

  Fragments arranged by size: 

                 361     189     125
 
PvuII CAG'CTG

Cuts at:      0      96     627     675 
   Size:         96     531      48

  Fragments arranged by size: 

                 531      96      48
 
SapI GCTCTTCn'nnn_

Cuts at:      0     480     675 
   Size:        480     195
 
SfcI C'TryA_G

Cuts at:      0     121     310     675 
   Size:        121     189     365

  Fragments arranged by size: 

                 365     189     121
 
SmlI C'TyrA_G

Cuts at:      0      64     361     675 
   Size:         64     297     314

  Fragments arranged by size: 

                 314     297      64
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      22     637     675 
   Size:         22     615      38

  Fragments arranged by size: 

                 615      38      22
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     589     621     675 
   Size:        589      32      54

  Fragments arranged by size: 

                 589      54      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     258     675 
   Size:        258     417
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     466     675 
   Size:        466     209
 

 Enzymes that do cut:

     AcuI     AlfI     ApaI    ApaLI     AvaI     BanI    BanII     BbsI
     BcgI     BlpI Bme1580I     BmrI     BpmI    BpuEI    BsaXI    BseRI
    BseYI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrFI   BstAPI   BstEII
    BstYI     BtsI     EaeI     EarI  Eco57MI EcoO109I     FspI    HaeII
    Hin4I   HincII     MmeI     MscI   MspA1I     NaeI   NgoMIV     NspI
     PasI    PflMI     PfoI   PspOMI     PstI    PvuII     SapI     SfcI
     SmlI    TaqII     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI    BciVI     BclI
    BfrBI     BglI    BglII    BmgBI     BmtI     BplI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BssHII    BssSI    BstBI
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MslI     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TatI     XbaI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com