Restriction map for
pF1KB8766
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI Aor51HI ApaLI AscI AseI
AsnI AspEI AspI AvaI AvrII
BalI BamHI BbeI BbsI BbvCI
BciVI BclI BfrI BglI BlnI
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Cfr10I ClaI CpoI
DraI DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV FauI FbaI FseI
HaeII Hin1I HincII HindII HindIII
HinfI HpaI KasI Ksp632I KspI
MaeIII MamI MfeI MluI MluNI
MroI MscI MslI MspA1I MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tsp45I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
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Acc65I 55 GGTACC G^GTACC
AccI 3 GTCTAC GT^[AC][GT]AC
AccII 126 CGCG CG^CG
AcsI 634 GAATTC [GA]^AATT[CT]
AocI 60 CCTGAGG CC^T[ACGT]AGG
ApaI 514 GGGCCC GGGCC^C
ApoI 634 GAATTC [GA]^AATT[CT]
Asp700I 486 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 55 GGTACC G^GTACC
AspHI 358 GAGCTC G[AT]GC[AT]^C
AviII 188 TGCGCA TGC^GCA
BanI 55 GGTACC G^G[CT][GA]CC
BbrPI 377 CACGTG CAC^GTG
BcgI 303 GCAGGTGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 290 AGATCT A^GATCT
BlpI 104 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 104 GCTTAGC GC^T[ACGT]AGC
BsaAI 377 CACGTG [CT]AC^GT[GA]
BseRI 436 GAGGAG GAGGAG(10/8)
BsgI 380 GTGCAG GTGCAG(16/14)
BsiEI 147 CGACCG CG[GA][CT]^CG
BsiHKAI 358 GAGCTC G[AT]GC[AT]^C
BsmAI 309 GTCTC GTCTC(1/5)
BsrI 193 ACTGG ACTGG(1/-1)
BstAPI 318 GCAGTTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 126 CGCG CG^CG
BstXI 44 CCACCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 290 AGATCT [GA]^GATC[CT]
Bsu36I 60 CCTGAGG CC^T[ACGT]AGG
CelII 104 GCTTAGC GC^T[ACGT]AGC
DpnII 291 GATC ^GATC
DraIII 666 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 119 CCACGG C^C[GA][CT]GG
Eco81I 60 CCTGAGG CC^T[ACGT]AGG
EcoRI 634 GAATTC G^AATTC
EcoT14I 110 CCAAGG C^C[AT][AT]GG
EcoT22I 446 ATGCAT ATGCA^T
FspI 188 TGCGCA TGC^GCA
HgaI 124 GACGC GACGC(5/10)
KpnI 55 GGTACC GGTAC^C
MboI 291 GATC ^GATC
MflI 290 AGATCT [GA]^GATC[CT]
MvnI 126 CGCG CG^CG
NdeII 291 GATC ^GATC
NsiI 446 ATGCAT ATGCA^T
NspI 281 ACATGC [GA]CATG^[CT]
PmaCI 377 CACGTG CAC^GTG
PmlI 377 CACGTG CAC^GTG
PpuMI 461 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 475 CTGCAG CTGCA^G
SacI 358 GAGCTC GAGCT^C
Sau3AI 291 GATC ^GATC
SexAI 667 ACCTGGT A^CC[AT]GGT
SfaNI 445 GATGC GCATC(5/9)
StuI 113 AGGCCT AGG^CCT
StyI 110 CCAAGG C^C[AT][AT]GG
TaqI 146 TCGA T^CGA
Tsp509I 635 AATT ^AATT
TthHB8I 146 TCGA T^CGA
XhoII 290 AGATCT [GA]^GATC[CT]
XmnI 486 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AvaII 462 GGTCC G^G[AT]CC
528 GGTCC
BbvI 270 GCTGC GCAGC(8/12)
327 GCTGC
BcnI 73 CCGGG CC^[GC]GG
337 CCGGG
BfaI 101 CTAG C^TAG
532 CTAG
BsiYI 558 CCTGGAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
662 CCTTCACCTGG
BslI 558 CCTGGAGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
662 CCTTCACCTGG
BspMI 185 ACCTGC ACCTGC(4/8)
303 GCAGGT
Cac8I 84 GCTGGC GC[ACGT]^[ACGT]GC
410 GCGTGC
CfoI 189 GCGC GCG^C
369 GCGC
DraII 461 AGGTCCT [GA]G^G[ACGT]CC[CT]
514 GGGCCCT
EcoO109I 461 AGGTCCT [GA]G^G[ACGT]CC[CT]
514 GGGCCCT
HapII 73 CCGG C^CGG
337 CCGG
HhaI 189 GCGC GCG^C
369 GCGC
HinP1I 189 GCGC G^CGC
369 GCGC
HpaII 73 CCGG C^CGG
337 CCGG
MaeI 101 CTAG C^TAG
532 CTAG
MboII 486 GAAGA GAAGA(8/7)
140 TCTTC
MseI 572 TTAA T^TAA
644 TTAA
MspI 73 CCGG C^CGG
337 CCGG
NciI 73 CCGGG CC^[GC]GG
337 CCGGG
NlaIV 55 GGTACC GG[ACGT]^[ACGT]CC
514 GGGCCC
SfcI 342 CTACAG C^T[GA][CT]AG
475 CTGCAG
Tru9I 572 TTAA T^TAA
644 TTAA
TseI 270 GCTGC G^C[AT]GC
327 GCTGC
TspRI 192 CACTG CA[GC]TG[ACGT][ACGT]^
553 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AfaI AluI BanII BmyI
BpmI BsaJI Bsp1286I BssKI BstF5I
BstNI Cfr13I DdeI EcoRII Fnu4HI
FokI HaeIII HphI ItaI MaeII
MnlI MvaI MwoI NlaIII RsaI
Sau96I ScrFI