Restriction map for pF1KB8766
 (Linear) (Six-base) MAPSORT of: KIBB8766.seq  Check: 4167  from: 1  to: 675



KIBB8766.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     294     675 
   Size:        294     381
 
AccI GT'mk_AC

Cuts at:      0       4     675 
   Size:          4     671
 
Acc65I G'GTAC_C

Cuts at:      0      55     675 
   Size:         55     620
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     242     675 
   Size:        242     433
 
ApaI G_GGCC'C

Cuts at:      0     518     675 
   Size:        518     157
 
ApoI r'AATT_y

Cuts at:      0     634     675 
   Size:        634      41
 
BanI G'GyrC_C

Cuts at:      0      55     675 
   Size:         55     620
 
BanII G_rGCy'C

Cuts at:      0      79     362     518     675 
   Size:         79     283     156     157

  Fragments arranged by size: 

                 283     157     156      79
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     292     326     675 
   Size:        292      34     349

  Fragments arranged by size: 

                 349     292      34
 
BfrBI ATG'CAT

Cuts at:      0     448     675 
   Size:        448     227
 
BglII A'GATC_T

Cuts at:      0     290     675 
   Size:        290     385
 
BlpI GC'TnA_GC

Cuts at:      0     105     675 
   Size:        105     570
 
Bme1580I G_kGCm'C

Cuts at:      0     206     518     675 
   Size:        206     312     157

  Fragments arranged by size: 

                 312     206     157
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     203     675 
   Size:        203     472
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     114     675 
   Size:        114     561
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     376     580     675 
   Size:        295      81     204      95

  Fragments arranged by size: 

                 295     204      95      81
 
BsaAI yAC'GTr

Cuts at:      0     379     675 
   Size:        379     296
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     451     675 
   Size:        451     224
 
BseYI C'CCAG_C

Cuts at:      0     154     675 
   Size:        154     521
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     401     675 
   Size:        401     274
 
BsiEI CG_ry'CG

Cuts at:      0     150     675 
   Size:        150     525
 
BsiHKAI G_wGCw'C

Cuts at:      0     362     675 
   Size:        362     313
 
Bsp1286I G_dGCh'C

Cuts at:      0      79     206     362     518     675 
   Size:         79     127     156     156     157

  Fragments arranged by size: 

                 157     156     156     127      79
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     194     294     675 
   Size:        194     100     381

  Fragments arranged by size: 

                 381     194     100
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     324     675 
   Size:        324     351
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      51     675 
   Size:         51     624
 
BstYI r'GATC_y

Cuts at:      0     290     675 
   Size:        290     385
 
Bsu36I CC'TnA_GG

Cuts at:      0      61     675 
   Size:         61     614
 
BtgI C'CryG_G

Cuts at:      0     119     675 
   Size:        119     556
 
BtsI GCAGTG_nn'

Cuts at:      0     552     675 
   Size:        552     123
 
DraIII CAC_nnn'GTG

Cuts at:      0     671     675 
   Size:        671       4
 
EcoICRI GAG'CTC

Cuts at:      0     360     675 
   Size:        360     315
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     242     295     376     580     675 
   Size:        242      53      81     204      95

  Fragments arranged by size: 

                 242     204      95      81      53
 
EcoO109I rG'GnC_Cy

Cuts at:      0     462     515     675 
   Size:        462      53     160

  Fragments arranged by size: 

                 462     160      53
 
EcoRI G'AATT_C

Cuts at:      0     634     675 
   Size:        634      41
 
FspI TGC'GCA

Cuts at:      0     190     675 
   Size:        190     485
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     292     324     675 
   Size:        292      32     351

  Fragments arranged by size: 

                 351     292      32
 
KpnI G_GTAC'C

Cuts at:      0      59     675 
   Size:         59     616
 
NsiI A_TGCA'T

Cuts at:      0     450     675 
   Size:        450     225
 
NspI r_CATG'y

Cuts at:      0     285     675 
   Size:        285     390
 
PasI CC'CwG_GG

Cuts at:      0      51     579     675 
   Size:         51     528      96

  Fragments arranged by size: 

                 528      96      51
 
PfoI T'CCnGG_A

Cuts at:      0     176     675 
   Size:        176     499
 
PmlI CAC'GTG

Cuts at:      0     379     675 
   Size:        379     296
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     226     258     675 
   Size:        226      32     417

  Fragments arranged by size: 

                 417     226      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     462     675 
   Size:        462     213
 
PspOMI G'GGCC_C

Cuts at:      0     514     675 
   Size:        514     161
 
PstI C_TGCA'G

Cuts at:      0     479     675 
   Size:        479     196
 
SacI G_AGCT'C

Cuts at:      0     362     675 
   Size:        362     313
 
SexAI A'CCwGG_T

Cuts at:      0     667     675 
   Size:        667       8
 
SfcI C'TryA_G

Cuts at:      0     342     475     675 
   Size:        342     133     200

  Fragments arranged by size: 

                 342     200     133
 
StuI AGG'CCT

Cuts at:      0     115     675 
   Size:        115     560
 
StyI C'CwwG_G

Cuts at:      0     110     675 
   Size:        110     565
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     200     232     675 
   Size:        200      32     443

  Fragments arranged by size: 

                 443     200      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     490     675 
   Size:        490     185
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     ApaI     ApoI     BanI    BanII
     BcgI    BfrBI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
    BsaAI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI
   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI   DraIII  EcoICRI
  Eco57MI EcoO109I    EcoRI     FspI    Hin4I     KpnI     NsiI     NspI
     PasI     PfoI     PmlI     PpiI    PpuMI   PspOMI     PstI     SacI
    SexAI     SfcI     StuI     StyI     TstI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI    BciVI     BclI
     BglI    BmgBI     BmtI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI
    BsaWI    BsaXI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I
    BtgZI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI    EcoNI    EcoRV     FalI     FseI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PciI    PflMI     PmeI    PshAI     PsiI    PspXI
     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TatI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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