Restriction map for pF1KB8763
 (Linear) (Six-base) MAPSORT of: KIBB8763.seq  Check: 1269  from: 1  to: 633



KIBB8763.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     397     633 
   Size:        397     236
 
AlwNI CAG_nnn'CTG

Cuts at:      0     528     633 
   Size:        528     105
 
ApaI G_GGCC'C

Cuts at:      0     248     492     633 
   Size:        248     244     141

  Fragments arranged by size: 

                 248     244     141
 
ApaLI G'TGCA_C

Cuts at:      0     256     382     633 
   Size:        256     126     251

  Fragments arranged by size: 

                 256     251     126
 
AvaI C'yCGr_G

Cuts at:      0     360     633 
   Size:        360     273
 
BanI G'GyrC_C

Cuts at:      0     295     468     633 
   Size:        295     173     165

  Fragments arranged by size: 

                 295     173     165
 
BanII G_rGCy'C

Cuts at:      0      56     248     290     492     616     633 
   Size:         56     192      42     202     124      17

  Fragments arranged by size: 

                 202     192     124      56      42      17
 
BbeI G_GCGC'C

Cuts at:      0     472     633 
   Size:        472     161
 
BglI GCCn_nnn'nGGC

Cuts at:      0      60     633 
   Size:         60     573
 
Bme1580I G_kGCm'C

Cuts at:      0     127     248     260     298     386     492     633 
   Size:        127     121      12      38      88     106     141

  Fragments arranged by size: 

                 141     127     121     106      88      38      12
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     249     633 
   Size:        249     384
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     472     633 
   Size:        472     161
 
Bpu10I CC'TnA_GC

Cuts at:      0     250     343     610     633 
   Size:        250      93     267      23

  Fragments arranged by size: 

                 267     250      93      23
 
BsaAI yAC'GTr

Cuts at:      0     462     633 
   Size:        462     171
 
BsaHI Gr'CG_yC

Cuts at:      0     224     469     633 
   Size:        224     245     164

  Fragments arranged by size: 

                 245     224     164
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     598     628     633 
   Size:        598      30       5

  Fragments arranged by size: 

                 598      30       5
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      69      78      81     633 
   Size:         69       9       3     552

  Fragments arranged by size: 

                 552      69       9       3
 
BseYI C'CCAG_C

Cuts at:      0     483     633 
   Size:        483     150
 
BsiEI CG_ry'CG

Cuts at:      0     121     399     517     633 
   Size:        121     278     118     116

  Fragments arranged by size: 

                 278     121     118     116
 
BsiHKAI G_wGCw'C

Cuts at:      0     260     386     458     616     633 
   Size:        260     126      72     158      17

  Fragments arranged by size: 

                 260     158     126      72      17
 
Bsp1286I G_dGCh'C

Cuts at:      0      56     127     248     260     290     298     386     458 
   Size:         56      71     121      12      30       8      88      72

Cuts at:    458     492     616     633 
   Size:         34     124      17

  Fragments arranged by size: 

                 124     121      88      72      71      56      34      30
                  17      12       8
 
BsrBI CCG'CTC

Cuts at:      0     273     633 
   Size:        273     360
 
BsrFI r'CCGG_y

Cuts at:      0     191     394     633 
   Size:        191     203     239

  Fragments arranged by size: 

                 239     203     191
 
BssHII G'CGCG_C

Cuts at:      0     109     204     212     279     633 
   Size:        109      95       8      67     354

  Fragments arranged by size: 

                 354     109      95      67       8
 
BssSI C'ACGA_G

Cuts at:      0     268     369     633 
   Size:        268     101     264

  Fragments arranged by size: 

                 268     264     101
 
BtgI C'CryG_G

Cuts at:      0      98     115     128     290     554     633 
   Size:         98      17      13     162     264      79

  Fragments arranged by size: 

                 264     162      98      79      17      13
 
DraIII CAC_nnn'GTG

Cuts at:      0     462     633 
   Size:        462     171
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      34     633 
   Size:         34     599
 
EaeI y'GGCC_r

Cuts at:      0      64     118     396     514     548     633 
   Size:         64      54     278     118      34      85

  Fragments arranged by size: 

                 278     118      85      64      54      34
 
EagI C'GGCC_G

Cuts at:      0     118     396     514     633 
   Size:        118     278     118     119

  Fragments arranged by size: 

                 278     119     118     118
 
EarI CTCTTCn'nnn_

Cuts at:      0      87     445     633 
   Size:         87     358     188

  Fragments arranged by size: 

                 358     188      87
 
EcoICRI GAG'CTC

Cuts at:      0     614     633 
   Size:        614      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     472     633 
   Size:        472     161
 
EcoO109I rG'GnC_Cy

Cuts at:      0     244     245     364     488     489     633 
   Size:        244       1     119     124       1     144

  Fragments arranged by size: 

                 244     144     124     119       1       1
 
FseI GG_CCGG'CC

Cuts at:      0     398     633 
   Size:        398     235
 
HaeII r_GCGC'y

Cuts at:      0     472     507     633 
   Size:        472      35     126

  Fragments arranged by size: 

                 472     126      35
 
KasI G'GCGC_C

Cuts at:      0     468     633 
   Size:        468     165
 
MscI TGG'CCA

Cuts at:      0     550     633 
   Size:        550      83
 
MslI CAynn'nnrTG

Cuts at:      0     379     410     633 
   Size:        379      31     223

  Fragments arranged by size: 

                 379     223      31
 
MspA1I CmG'CkG

Cuts at:      0     100     117     130     324     495     519     633 
   Size:        100      17      13     194     171      24     114

  Fragments arranged by size: 

                 194     171     114     100      24      17      13
 
NaeI GCC'GGC

Cuts at:      0     396     633 
   Size:        396     237
 
NarI GG'CG_CC

Cuts at:      0     469     633 
   Size:        469     164
 
NgoMIV G'CCGG_C

Cuts at:      0     394     633 
   Size:        394     239
 
NotI GC'GGCC_GC

Cuts at:      0     514     633 
   Size:        514     119
 
NspI r_CATG'y

Cuts at:      0     483     633 
   Size:        483     150
 
PfoI T'CCnGG_A

Cuts at:      0     218     633 
   Size:        218     415
 
PpuMI rG'GwC_Cy

Cuts at:      0     364     633 
   Size:        364     269
 
PspOMI G'GGCC_C

Cuts at:      0     244     488     633 
   Size:        244     244     145

  Fragments arranged by size: 

                 244     244     145
 
PstI C_TGCA'G

Cuts at:      0     524     633 
   Size:        524     109
 
SacI G_AGCT'C

Cuts at:      0     616     633 
   Size:        616      17
 
SacII CC_GC'GG

Cuts at:      0     101     118     131     633 
   Size:        101      17      13     502

  Fragments arranged by size: 

                 502     101      17      13
 
SanDI GG'GwC_CC

Cuts at:      0     364     633 
   Size:        364     269
 
SexAI A'CCwGG_T

Cuts at:      0      14     633 
   Size:         14     619
 
SfcI C'TryA_G

Cuts at:      0     520     633 
   Size:        520     113
 
SfoI GGC'GCC

Cuts at:      0     470     633 
   Size:        470     163
 
SphI G_CATG'C

Cuts at:      0     483     633 
   Size:        483     150
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     216     633 
   Size:        216     417
 

 Enzymes that do cut:

     AlfI    AlwNI     ApaI    ApaLI     AvaI     BanI    BanII     BbeI
     BglI Bme1580I     BmrI     BpmI   Bpu10I    BsaAI    BsaHI    BsaXI
    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BsrBI    BsrFI   BssHII
    BssSI     BtgI   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI
  Eco57MI EcoO109I     FseI    HaeII     KasI     MscI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NotI     NspI     PfoI    PpuMI   PspOMI
     PstI     SacI    SacII    SanDI    SexAI     SfcI     SfoI     SphI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI    BpuEI
     BsaI    BsaBI    BsaWI     BsgI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrDI    BsrGI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
     EciI    EcoNI    EcoRI    EcoRV     FalI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     NcoI
     NdeI     NheI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    PvuII    RsrII     SalI     SapI     SbfI     ScaI     SfiI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI
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