Restriction map for pF1KB8759
##################################
# NO CUT ENZYMES (  177 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcyI        
AfaI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BciVI       BclI        
BcnI        BfrI        BglI        BlnI        BlpI        
BmyI        Bpu1102I    BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HapII       HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       KasI        
KpnI        Ksp632I     KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MslI        
MspI        MunI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
PvuI        RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI          376  GTAGAC               GT^[AC][GT]AC
AciI          325  GCGG                 CCGC(-3/-1)
AclI          200  AACGTT               AA^CGTT
AlwNI         511  CAGGTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          213  CCTTAGG              CC^T[ACGT]AGG
AvaII         336  GGACC                G^G[AT]CC
BbsI          591  GTCTTC               GAAGAC(2/6)
BbvI          245  GCTGC                GCAGC(8/12)
BcgI          586  GCAGGGTCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          458  CTAG                 C^TAG
BpuAI         591  GTCTTC               GAAGAC(2/6)
BsaAI          79  TACGTA               [CT]AC^GT[GA]
BsaJI         101  CCCAGG               C^C[ACGT][ACGT]GG
BseRI         406  GAGGAG               GAGGAG(10/8)
BsmFI         182  GGGAC                GGGAC(10/14)
BspMI         510  GCAGGT               ACCTGC(4/8)
BsrDI         541  CATTGC               GCAATG(2/0)
BsrI          299  ACTGG                ACTGG(1/-1)
Bsu36I        213  CCTTAGG              CC^T[ACGT]AGG
Cac8I         516  GCTGGC               GC[ACGT]^[ACGT]GC
Cfr13I        336  GGACC                G^G[ACGT]CC
DraII         335  AGGACCC              [GA]G^G[ACGT]CC[CT]
Eco81I        213  CCTTAGG              CC^T[ACGT]AGG
EcoNI         224  CCTTCTACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      335  AGGACCC              [GA]G^G[ACGT]CC[CT]
Fnu4HI        245  GCTGC                GC^[ACGT]GC
HaeIII        489  GGCC                 GG^CC
HgaI          158  GACGC                GACGC(5/10)
HphI           40  GGTGA                GGTGA(8/7)
ItaI          245  GCTGC                GC^[ACGT]GC
MaeI          458  CTAG                 C^TAG
MspA1I        532  CAGCTG               C[AC]G^C[GT]G
NspI          545  GCATGT               [GA]CATG^[CT]
PleI           97  GACTC                GAGTC(4/5)
PpuMI         335  AGGACCC              [GA]G^G[AT]CC[CT]
Psp1406I      200  AACGTT               AA^CGTT
PstI          188  CTGCAG               CTGCA^G
PvuII         532  CAGCTG               CAG^CTG
Sau96I        336  GGACC                G^G[ACGT]CC
SexAI         418  ACCTGGT              A^CC[AT]GGT
SnaBI          79  TACGTA               TAC^GTA
SpeI          457  ACTAGT               A^CTAGT
TfiI          288  GAATC                G^A[AT]TC
TseI          245  GCTGC                G^C[AT]GC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI           91  AAATTT               [GA]^AATT[CT]
              310  GAATTT              
ApoI           91  AAATTT               [GA]^AATT[CT]
              310  GAATTT              
BglII         141  AGATCT               A^GATCT
              176  AGATCT              
BssKI         102  CCAGG                ^CC[ACGT]GG
              419  CCTGG               
BstF5I        267  GGATG                GGATG(2/0)
              327  GGATG               
BstNI         102  CCAGG                CC^[AT]GG
              419  CCTGG               
BstYI         141  AGATCT               [GA]^GATC[CT]
              176  AGATCT              
EcoRII        102  CCAGG                ^CC[AT]GG
              419  CCTGG               
FokI          267  GGATG                GGATG(9/13)
              327  GGATG               
HinfI          97  GACTC                G^A[ACGT]TC
              288  GAATC               
MaeII          80  ACGT                 A^CGT
              201  ACGT                
MboII         496  GAAGA                GAAGA(8/7)
              592  TCTTC               
MflI          141  AGATCT               [GA]^GATC[CT]
              176  AGATCT              
MseI           22  TTAA                 T^TAA
              133  TTAA                
MvaI          102  CCAGG                CC^[AT]GG
              419  CCTGG               
NlaIII        426  CATG                 CATG^
              546  CATG                
NlaIV         336  GGACCC               GG[ACGT]^[ACGT]CC
              577  GGCTCC              
ScrFI         102  CCAGG                CC^[ACGT]GG
              419  CCTGG               
TaqI          138  TCGA                 T^CGA
              284  TCGA                
Tru9I          22  TTAA                 T^TAA
              133  TTAA                
Tsp509I        92  AATT                 ^AATT
              311  AATT                
TthHB8I       138  TCGA                 T^CGA
              284  TCGA                
XhoII         141  AGATCT               [GA]^GATC[CT]
              176  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AluI        BpmI        BsiYI       BslI        DdeI        
DpnII       MaeIII      MboI        MnlI        NdeII       
Sau3AI      SfcI        Tsp45I      TspRI       
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